Restriction map for
pF1KB6188
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BciVI BfrI
BlnI BsaAI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
KspI MluNI MroI MscI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AclI 448 AACGTT AA^CGTT
AcyI 1447 GGCGCC G[GA]^CG[CT]C
AfaI 1078 GTAC GT^AC
AflIII 40 ACGCGT A^C[GA][CT]GT
AlwI 1587 GATCC GGATC(4/5)
AvaI 1905 CCCGGG C^[CT]CG[GA]G
BbeI 1447 GGCGCC GGCGC^C
BbvCI 297 CCTCAGC CCTCAGC(-5/-2)
BcgI 1704 GCAACTATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 995 TGATCA T^GATCA
BglII 249 AGATCT A^GATCT
BsaHI 1447 GGCGCC G[GA]^CG[CT]C
BsmBI 316 CGTCTC CGTCTC(1/5)
BsoBI 1905 CCCGGG C^[CT]CG[GA]G
BsrBI 926 CCGCTC CCGCTC(-3/-3)
BstXI 1662 CCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 122 TGGCCG [CT]^GGCC[GA]
EcoNI 846 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 995 TGATCA T^GATCA
Hin1I 1447 GGCGCC G[GA]^CG[CT]C
HincII 520 GTCAAC GT[CT]^[GA]AC
HindII 520 GTCAAC GT[CT]^[GA]AC
KasI 1447 GGCGCC G^GCGCC
MaeIII 1635 GTCAC ^GT[ACGT]AC
MfeI 1751 CAATTG C^AATTG
MluI 40 ACGCGT A^CGCGT
MseI 1646 TTAA T^TAA
MslI 1953 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1699 CAGCTG C[AC]G^C[GT]G
MunI 1751 CAATTG C^AATTG
NarI 1447 GGCGCC GG^CGCC
Psp1406I 448 AACGTT AA^CGTT
PvuII 1699 CAGCTG CAG^CTG
RsaI 1078 GTAC GT^AC
SacI 1159 GAGCTC GAGCT^C
ScaI 1077 AGTACT AGT^ACT
SfiI 2067 GGCCCACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1447 GGCGCC GGC^GCC
SmaI 1905 CCCGGG CCC^GGG
SmlI 1162 CTCAAG C^T[CT][GA]AG
Tru9I 1646 TTAA T^TAA
Tsp45I 1635 GTCAC ^GT[GC]AC
Tsp509I 1752 AATT ^AATT
XcmI 1844 CCAAGATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1905 CCCGGG C^CCGGG
XmaI 1905 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 41 CGCG CG^CG
314 CGCG
AlwNI 1051 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
2048 CAGTCTCTG
AspHI 361 GTGCTC G[AT]GC[AT]^C
1159 GAGCTC
BanI 387 GGCACC G^G[CT][GA]CC
1447 GGCGCC
BfaI 112 CTAG C^TAG
1474 CTAG
BlpI 18 GCTCAGC GC^T[ACGT]AGC
63 GCTTAGC
Bpu1102I 18 GCTCAGC GC^T[ACGT]AGC
63 GCTTAGC
BsaBI 1926 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1962 GATTCACATC
BsaI 1469 GAGACC GGTCTC(1/5)
1882 GAGACC
BsiHKAI 361 GTGCTC G[AT]GC[AT]^C
1159 GAGCTC
BsmI 1110 GAATGC GAATGC(1/-1)
1738 GCATTC
BspMI 59 ACCTGC ACCTGC(4/8)
1802 ACCTGC
BsrDI 1218 CATTGC GCAATG(2/0)
2024 GCAATG
BsrFI 882 GCCGGC [GA]^CCGG[CT]
1502 GCCGGC
BstUI 41 CGCG CG^CG
314 CGCG
BstYI 249 AGATCT [GA]^GATC[CT]
1586 AGATCC
CelII 18 GCTCAGC GC^T[ACGT]AGC
63 GCTTAGC
CfoI 1448 GCGC GCG^C
1563 GCGC
Cfr10I 882 GCCGGC [GA]^CCGG[CT]
1502 GCCGGC
FauI 925 CCCGC CCCGC(4/6)
951 CCCGC
HaeII 1447 GGCGCC [GA]GCGC^[CT]
1562 AGCGCC
HhaI 1448 GCGC GCG^C
1563 GCGC
HinP1I 1448 GCGC G^CGC
1563 GCGC
MaeI 112 CTAG C^TAG
1474 CTAG
MaeII 191 ACGT A^CGT
449 ACGT
MamI 1926 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1962 GATTCACATC
MflI 249 AGATCT [GA]^GATC[CT]
1586 AGATCC
MvnI 41 CGCG CG^CG
314 CGCG
NaeI 882 GCCGGC GCC^GGC
1502 GCCGGC
NcoI 223 CCATGG C^CATGG
1868 CCATGG
NgoMIV 882 GCCGGC G^CCGGC
1502 GCCGGC
PpuMI 581 AGGTCCT [GA]G^G[AT]CC[CT]
1366 GGGTCCT
PshAI 76 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1610 GACAGAAGTC
StuI 530 AGGCCT AGG^CCT
2075 AGGCCT
TaqI 864 TCGA T^CGA
1934 TCGA
TthHB8I 864 TCGA T^CGA
1934 TCGA
XhoII 249 AGATCT [GA]^GATC[CT]
1586 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AocI ApaI AspI
AvaII BanII BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PstI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI TspRI
Tth111I