Restriction map for
pF1KB6213
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FauI FseI FspI Hin1I
HpaI KasI KspI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AgeI 941 ACCGGT A^CCGGT
AocI 1498 CCTAAGG CC^T[ACGT]AGG
BalI 1823 TGGCCA TGG^CCA
BclI 426 TGATCA T^GATCA
BglII 1566 AGATCT A^GATCT
BpmI 1996 CTGGAG CTGGAG(16/14)
BsaWI 941 ACCGGT [AT]^CCGG[AT]
BspMI 1953 ACCTGC ACCTGC(4/8)
BstAPI 664 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1498 CCTAAGG CC^T[ACGT]AGG
Eco81I 1498 CCTAAGG CC^T[ACGT]AGG
FbaI 426 TGATCA T^GATCA
HincII 747 GTCGAC GT[CT]^[GA]AC
HindII 747 GTCGAC GT[CT]^[GA]AC
HindIII 648 AAGCTT A^AGCTT
MaeII 32 ACGT A^CGT
MluNI 1823 TGGCCA TGG^CCA
MscI 1823 TGGCCA TGG^CCA
NcoI 701 CCATGG C^CATGG
PflMI 354 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 941 ACCGGT A^CCGGT
PleI 1512 GACTC GAGTC(4/5)
SacI 85 GAGCTC GAGCT^C
SalI 747 GTCGAC G^TCGAC
SexAI 65 ACCTGGT A^CC[AT]GGT
SfaNI 1798 GATGC GCATC(5/9)
StuI 1729 AGGCCT AGG^CCT
Tsp509I 278 AATT ^AATT
Van91I 354 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 743 CCATGTCGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
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Acc65I 756 GGTACC G^GTACC
1609 GGTACC
AccI 747 GTCGAC GT^[AC][GT]AC
1000 GTCTAC
AflIII 11 ACATGT A^C[GA][CT]GT
923 ACATGT
AlwI 468 GATCC GGATC(4/5)
1184 GGATC
AlwNI 355 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
665 CAGCTGCTG
ApaI 1143 GGGCCC GGGCC^C
1841 GGGCCC
Asp700I 259 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
852 GAAGGACTTC
Asp718I 756 GGTACC G^GTACC
1609 GGTACC
AspHI 85 GAGCTC G[AT]GC[AT]^C
1482 GTGCTC
AvaII 537 GGACC G^G[AT]CC
1410 GGACC
BbsI 411 GAAGAC GAAGAC(2/6)
1212 GAAGAC
BciVI 705 GGATAC GTATCC(6/5)
1833 GGATAC
BglI 808 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1321 GCCAACAGGGC
BpuAI 411 GAAGAC GAAGAC(2/6)
1212 GAAGAC
BseRI 82 GAGGAG GAGGAG(10/8)
829 GAGGAG
BsiHKAI 85 GAGCTC G[AT]GC[AT]^C
1482 GTGCTC
BspLU11I 11 ACATGT A^CATGT
923 ACATGT
BsrFI 560 ACCGGC [GA]^CCGG[CT]
941 ACCGGT
BstF5I 1966 CATCC GGATG(2/0)
786 GGATG
BstXI 1331 CCAAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1805 CCAATCCTGTGG
Cfr10I 560 ACCGGC [GA]^CCGG[CT]
941 ACCGGT
DsaI 701 CCATGG C^C[GA][CT]GG
1250 CCACGG
EaeI 5 CGGCCA [CT]^GGCC[GA]
1823 TGGCCA
EarI 48 CTCTTC CTCTTC(1/4)
199 GAAGAG
EcoRV 343 GATATC GAT^ATC
682 GATATC
EcoT14I 701 CCATGG C^C[AT][AT]GG
1423 CCAAGG
FokI 1966 CATCC GGATG(9/13)
786 GGATG
HaeII 236 AGCGCC [GA]GCGC^[CT]
1318 AGCGCC
HgaI 1397 GACGC GACGC(5/10)
1776 GCGTC
KpnI 756 GGTACC GGTAC^C
1609 GGTACC
Ksp632I 48 CTCTTC CTCTTC(1/4)
199 GAAGAG
MaeIII 322 GTGAC ^GT[ACGT]AC
894 GTCAC
PpuMI 536 GGGACCC [GA]G^G[AT]CC[CT]
1409 GGGACCT
StyI 701 CCATGG C^C[AT][AT]GG
1423 CCAAGG
Tsp45I 322 GTGAC ^GT[GC]AC
894 GTCAC
XmnI 259 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
852 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AvaI BanI
BanII BbvI BcnI BfaI BmyI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoNI EcoO109I
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PvuII RsaI Sau3AI Sau96I ScrFI
SmlI TaqI TfiI TseI TspRI
TthHB8I XhoII