Restriction map for
pF1KB6227
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AflII AflIII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AviII AvrII
BanII BbeI BbrPI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI KspI MaeI MfeI MluI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AhdI 1898 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1753 CCTGAGG CC^T[ACGT]AGG
Asp700I 756 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1898 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 15 CCCGGG C^[CT]CG[GA]G
BalI 47 TGGCCA TGG^CCA
BanI 230 GGTGCC G^G[CT][GA]CC
BclI 1920 TGATCA T^GATCA
BglI 932 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1338 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 36 GTCTC GTCTC(1/5)
BsmBI 35 CGTCTC CGTCTC(1/5)
BsoBI 15 CCCGGG C^[CT]CG[GA]G
BspMI 400 ACCTGC ACCTGC(4/8)
BsrBI 645 CCGCTC CCGCTC(-3/-3)
BsrDI 423 GCAATG GCAATG(2/0)
BsrFI 807 GCCGGC [GA]^CCGG[CT]
BssHII 121 GCGCGC G^CGCGC
BstAPI 149 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1733 CCAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1753 CCTGAGG CC^T[ACGT]AGG
Cfr10I 807 GCCGGC [GA]^CCGG[CT]
DraIII 94 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1898 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 471 GAAGAG CTCTTC(1/4)
Eco81I 1753 CCTGAGG CC^T[ACGT]AGG
EcoNI 1107 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 62 CCCGC CCCGC(4/6)
FbaI 1920 TGATCA T^GATCA
HaeII 72 GGCGCT [GA]GCGC^[CT]
Ksp632I 471 GAAGAG CTCTTC(1/4)
MamI 1338 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 47 TGGCCA TGG^CCA
MscI 47 TGGCCA TGG^CCA
NaeI 807 GCCGGC GCC^GGC
NcoI 1151 CCATGG C^CATGG
NgoMIV 807 GCCGGC G^CCGGC
NspI 1779 GCATGC [GA]CATG^[CT]
PshAI 713 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 147 CTGCAG CTGCA^G
ScaI 851 AGTACT AGT^ACT
SmaI 15 CCCGGG CCC^GGG
SphI 1779 GCATGC GCATG^C
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
XmnI 756 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 120 CGCG CG^CG
122 CGCG
AccIII 870 TCCGGA T^CCGGA
1790 TCCGGA
AclI 412 AACGTT AA^CGTT
1660 AACGTT
AcyI 33 GGCGTC G[GA]^CG[CT]C
1573 GGCGTC
AlwNI 150 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
444 CAGCCTCTG
BamHI 558 GGATCC G^GATCC
769 GGATCC
BbsI 964 GAAGAC GAAGAC(2/6)
1627 GAAGAC
BbvCI 75 GCTGAGG CCTCAGC(-5/-2)
496 GCTGAGG
BpmI 1502 CTCCAG CTGGAG(16/14)
1813 CTGGAG
BpuAI 964 GAAGAC GAAGAC(2/6)
1627 GAAGAC
BsaHI 33 GGCGTC G[GA]^CG[CT]C
1573 GGCGTC
BsaWI 870 TCCGGA [AT]^CCGG[AT]
1790 TCCGGA
BseAI 870 TCCGGA T^CCGGA
1790 TCCGGA
BspEI 870 TCCGGA T^CCGGA
1790 TCCGGA
BssSI 94 CACGAG CACGAG(-5/-1)
1892 CACGAG
BstNI 293 CCAGG CC^[AT]GG
1484 CCAGG
BstUI 120 CGCG CG^CG
122 CGCG
DsaI 29 CCACGG C^C[GA][CT]GG
1151 CCATGG
EaeI 47 TGGCCA [CT]^GGCC[GA]
809 CGGCCA
EcoRII 293 CCAGG ^CC[AT]GG
1484 CCAGG
EcoT14I 1151 CCATGG C^C[AT][AT]GG
1685 CCAAGG
Hin1I 33 GGCGTC G[GA]^CG[CT]C
1573 GGCGTC
HincII 1258 GTCAAC GT[CT]^[GA]AC
1975 GTCAAC
HindII 1258 GTCAAC GT[CT]^[GA]AC
1975 GTCAAC
MroI 870 TCCGGA T^CCGGA
1790 TCCGGA
MspA1I 1204 CAGCTG C[AC]G^C[GT]G
1924 CAGCTG
MvaI 293 CCAGG CC^[AT]GG
1484 CCAGG
MvnI 120 CGCG CG^CG
122 CGCG
Psp1406I 412 AACGTT AA^CGTT
1660 AACGTT
PvuII 1204 CAGCTG CAG^CTG
1924 CAGCTG
SfcI 147 CTGCAG C^T[GA][CT]AG
722 CTGTAG
StyI 1151 CCATGG C^C[AT][AT]GG
1685 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI AspHI AvaII
BbvI BcnI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII