################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BcgI BciVI BclI BcnI BfrI BglII BlnI BpmI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 37 CCGC CCGC(-3/-1) AflIII 240 ACACGT A^C[GA][CT]GT AspHI 244 GTGCTC G[AT]GC[AT]^C AvaII 66 GGACC G^G[AT]CC BbrPI 241 CACGTG CAC^GTG BbsI 279 GAAGAC GAAGAC(2/6) BbvCI 286 GCTGAGG CCTCAGC(-5/-2) BfaI 43 CTAG C^TAG BglI 430 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 100 GCTCAGC GC^T[ACGT]AGC BmyI 244 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 100 GCTCAGC GC^T[ACGT]AGC BpuAI 279 GAAGAC GAAGAC(2/6) BsaAI 241 CACGTG [CT]AC^GT[GA] BsiHKAI 244 GTGCTC G[AT]GC[AT]^C BsiYI 69 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 69 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 244 GTGCTC G[AGT]GC[ACT]^C BspDI 250 ATCGAT AT^CGAT BsrDI 96 CATTGC GCAATG(2/0) BsrI 262 ACTGG ACTGG(1/-1) BstF5I 291 GGATG GGATG(2/0) Cac8I 372 GCACGC GC[ACGT]^[ACGT]GC CelII 100 GCTCAGC GC^T[ACGT]AGC CfoI 4 GCGC GCG^C Cfr13I 66 GGACC G^G[ACGT]CC ClaI 250 ATCGAT AT^CGAT EarI 359 CTCTTC CTCTTC(1/4) FokI 291 GGATG GGATG(9/13) HhaI 4 GCGC GCG^C HinP1I 4 GCGC G^CGC Ksp632I 359 CTCTTC CTCTTC(1/4) MaeI 43 CTAG C^TAG MseI 14 TTAA T^TAA PflMI 69 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 405 GAGTC GAGTC(4/5) PmaCI 241 CACGTG CAC^GTG PmlI 241 CACGTG CAC^GTG SapI 358 GCTCTTC GCTCTTC(1/4) Sau96I 66 GGACC G^G[ACGT]CC SfaNI 292 GATGC GCATC(5/9) SmlI 52 CTCAAG C^T[CT][GA]AG TaqI 251 TCGA T^CGA Tru9I 14 TTAA T^TAA Tsp509I 182 AATT ^AATT TspRI 194 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 251 TCGA T^CGA Van91I 69 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AfaI 115 GTAC GT^AC 267 GTAC BsmFI 190 GTCCC GGGAC(10/14) 214 GTCCC BstXI 122 CCAAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 332 CCAAGCAGATGG EcoT14I 137 CCAAGG C^C[AT][AT]GG 296 CCAAGG HaeIII 387 GGCC GG^CC 459 GGCC MslI 249 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 393 CATGGAAATG MspA1I 61 CAGCTG C[AC]G^C[GT]G 284 CAGCTG PvuII 61 CAGCTG CAG^CTG 284 CAGCTG RsaI 115 GTAC GT^AC 267 GTAC StyI 137 CCAAGG C^C[AT][AT]GG 296 CCAAGG TfiI 30 GAATC G^A[AT]TC 414 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BbvI BsaJI BssKI BstNI DdeI EcoRII Fnu4HI HinfI ItaI MaeII MboII MnlI MvaI MwoI NlaIII ScrFI TseI