##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       Cfr10I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        HaeII       HapII       HgaI        
Hin1I       HincII      HindII      HinfI       HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI          343  GTCTAC               GT^[AC][GT]AC
AciI            9  CCGC                 CCGC(-3/-1)
AviII         314  TGCGCA               TGC^GCA
BanII         290  GGGCTC               G[GA]GC[CT]^C
BbsI          180  GAAGAC               GAAGAC(2/6)
BmyI          290  GGGCTC               G[AGT]GC[ACT]^C
BpuAI         180  GAAGAC               GAAGAC(2/6)
BsaJI         140  CCCAGG               C^C[ACGT][ACGT]GG
BseRI          66  GAGGAG               GAGGAG(10/8)
BsgI          329  CTGCAC               GTGCAG(16/14)
BsiYI         123  CCTCAATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          123  CCTCAATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI           6  GTCCC                GGGAC(10/14)
Bsp1286I      290  GGGCTC               G[AGT]GC[ACT]^C
BspMI          94  ACCTGC               ACCTGC(4/8)
BsrDI         321  CATTGC               GCAATG(2/0)
BstF5I        276  GGATG                GGATG(2/0)
BstXI         326  CCACTGCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          315  GCGC                 GCG^C
FauI            8  CCCGC                CCCGC(4/6)
FokI          276  GGATG                GGATG(9/13)
FspI          314  TGCGCA               TGC^GCA
HhaI          315  GCGC                 GCG^C
HinP1I        315  GCGC                 G^CGC
HindIII       107  AAGCTT               A^AGCTT
MboII         180  GAAGA                GAAGA(8/7)
MslI          121  CACCTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        355  CAGCTG               C[AC]G^C[GT]G
NspI          311  ACATGC               [GA]CATG^[CT]
PvuII         355  CAGCTG               CAG^CTG
SfaNI         299  GCATC                GCATC(5/9)
SmlI          262  CTCAAG               C^T[CT][GA]AG
StuI          143  AGGCCT               AGG^CCT
TaqI          302  TCGA                 T^CGA
TthHB8I       302  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwI          117  GGATC                GGATC(4/5)
              230  GGATC               
AvaII         168  GGACC                G^G[AT]CC
              192  GGTCC               
DpnII         118  GATC                 ^GATC
              231  GATC                
HphI          177  GGTGA                GGTGA(8/7)
              120  TCACC               
MboI          118  GATC                 ^GATC
              231  GATC                
NdeII         118  GATC                 ^GATC
              231  GATC                
Sau3AI        118  GATC                 ^GATC
              231  GATC                
Tsp509I       206  AATT                 ^AATT
              270  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AluI        BbvI        BsrI        BssKI       BstNI       
Cfr13I      EcoRII      Fnu4HI      HaeIII      ItaI        
MnlI        MvaI        MwoI        NlaIII      PstI        
Sau96I      ScrFI       SfcI        TseI        TspRI