Restriction map for
pF1KB6289
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# NO CUT ENZYMES ( 111 enzymes) #
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AccI AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BpuAI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KspI MluI MroI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 1134 GGTACC G^GTACC
AcsI 313 AAATTC [GA]^AATT[CT]
AflIII 878 ACATGT A^C[GA][CT]GT
AhdI 982 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1159 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 313 AAATTC [GA]^AATT[CT]
Asp718I 1134 GGTACC G^GTACC
AspEI 982 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BlpI 1761 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1761 GCTCAGC GC^T[ACGT]AGC
BsaAI 789 TACGTG [CT]AC^GT[GA]
BsaBI 445 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1030 CGACCG CG[GA][CT]^CG
BsmBI 892 GAGACG CGTCTC(1/5)
BspHI 1214 TCATGA T^CATGA
BspLU11I 878 ACATGT A^CATGT
BspMI 432 ACCTGC ACCTGC(4/8)
BsrBI 703 GAGCGG CCGCTC(-3/-3)
BsrFI 1625 GCCGGT [GA]^CCGG[CT]
BstBI 560 TTCGAA TT^CGAA
CelII 1761 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1625 GCCGGT [GA]^CCGG[CT]
DrdI 877 GACATGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1114 CCACGG C^C[GA][CT]GG
Eam1105I 982 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2226 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 679 GCGTC GACGC(5/10)
KpnI 1134 GGTACC GGTAC^C
MamI 445 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 997 CAATTG C^AATTG
MunI 997 CAATTG C^AATTG
NheI 1272 GCTAGC G^CTAGC
NspI 878 ACATGT [GA]CATG^[CT]
NspV 560 TTCGAA TT^CGAA
RcaI 1214 TCATGA T^CATGA
SapI 1680 GCTCTTC GCTCTTC(1/4)
ScaI 1699 AGTACT AGT^ACT
SfuI 560 TTCGAA TT^CGAA
SspI 247 AATATT AAT^ATT
XcmI 673 CCATCAGCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 894 GACGTC GACGT^C
2092 GACGTC
AcyI 894 GACGTC G[GA]^CG[CT]C
2092 GACGTC
AvaI 1003 CCCGAG C^[CT]CG[GA]G
1833 CCCGAG
BalI 227 TGGCCA TGG^CCA
725 TGGCCA
BbvCI 2190 CCTCAGC CCTCAGC(-5/-2)
1276 GCTGAGG
BsaHI 894 GACGTC G[GA]^CG[CT]C
2092 GACGTC
BsoBI 1003 CCCGAG C^[CT]CG[GA]G
1833 CCCGAG
BstAPI 268 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1644 GCATCTACTGC
BstXI 140 CCAAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1427 CCATCAAAGTGG
CfoI 765 GCGC GCG^C
1085 GCGC
HhaI 765 GCGC GCG^C
1085 GCGC
Hin1I 894 GACGTC G[GA]^CG[CT]C
2092 GACGTC
HinP1I 765 GCGC G^CGC
1085 GCGC
MluNI 227 TGGCCA TGG^CCA
725 TGGCCA
MscI 227 TGGCCA TGG^CCA
725 TGGCCA
PflMI 1158 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1773 CCAGGAAGTGG
PstI 434 CTGCAG CTGCA^G
1304 CTGCAG
PvuII 176 CAGCTG CAG^CTG
327 CAGCTG
SexAI 962 ACCAGGT A^CC[AT]GGT
1130 ACCTGGT
StuI 134 AGGCCT AGG^CCT
2160 AGGCCT
TaqI 561 TCGA T^CGA
667 TCGA
TthHB8I 561 TCGA T^CGA
667 TCGA
Van91I 1158 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1773 CCAGGAAGTGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII