################################## # NO CUT ENZYMES ( 202 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII HpaI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 34 GCGG CCGC(-3/-1) AfaI 252 GTAC GT^AC AvaII 405 GGTCC G^G[AT]CC BbsI 346 GAAGAC GAAGAC(2/6) BcnI 113 CCCGG CC^[GC]GG BmyI 110 GTGCCC G[AGT]GC[ACT]^C BpuAI 346 GAAGAC GAAGAC(2/6) BsmFI 406 GTCCC GGGAC(10/14) Bsp1286I 110 GTGCCC G[AGT]GC[ACT]^C BspMI 364 ACCTGC ACCTGC(4/8) BstXI 431 CCAAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 147 AGATCC [GA]^GATC[CT] Cac8I 463 GCCTGC GC[ACGT]^[ACGT]GC Cfr13I 405 GGTCC G^G[ACGT]CC HapII 114 CCGG C^CGG HincII 91 GTTGAC GT[CT]^[GA]AC HindII 91 GTTGAC GT[CT]^[GA]AC HinfI 26 GAGTC G^A[ACGT]TC HpaII 114 CCGG C^CGG MaeIII 316 GTTAC ^GT[ACGT]AC MflI 147 AGATCC [GA]^GATC[CT] MslI 396 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 398 CAGCTG C[AC]G^C[GT]G MspI 114 CCGG C^CGG NciI 113 CCCGG CC^[GC]GG NlaIII 55 CATG CATG^ NlaIV 405 GGTCCC GG[ACGT]^[ACGT]CC PleI 26 GAGTC GAGTC(4/5) PvuII 398 CAGCTG CAG^CTG RsaI 252 GTAC GT^AC Sau96I 405 GGTCC G^G[ACGT]CC SfaNI 460 GATGC GCATC(5/9) Tsp509I 183 AATT ^AATT TspRI 351 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 147 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 299 AGCT AG^CT 399 AGCT BsaI 361 GAGACC GGTCTC(1/5) 427 GAGACC BsmAI 361 GAGAC GTCTC(1/5) 427 GAGAC BstF5I 132 CATCC GGATG(2/0) 177 CATCC BstNI 30 CCTGG CC^[AT]GG 125 CCAGG EcoRII 30 CCTGG ^CC[AT]GG 125 CCAGG FokI 132 CATCC GGATG(9/13) 177 CATCC HphI 424 GGTGA GGTGA(8/7) 229 TCACC MseI 44 TTAA T^TAA 451 TTAA MvaI 30 CCTGG CC^[AT]GG 125 CCAGG SfcI 287 CTACAG C^T[GA][CT]AG 356 CTACAG Tru9I 44 TTAA T^TAA 451 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AlwI BssKI DpnII MboI MboII MnlI NdeII Sau3AI ScrFI