Restriction map for
pF1KB6159
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BclI BcnI BfrI
BglI BlnI BpuAI BsaBI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BspDI BspEI BspHI
BspMI BsrDI BsrFI BssHII BstBI
BstEII BstPI BstUI Bsu36I CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 177 GGTACC G^GTACC
AccI 866 GTATAC GT^[AC][GT]AC
AcsI 432 GAATTC [GA]^AATT[CT]
ApoI 432 GAATTC [GA]^AATT[CT]
Asp700I 950 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 177 GGTACC G^GTACC
AspI 233 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 777 CCCGAG C^[CT]CG[GA]G
BanI 177 GGTACC G^G[CT][GA]CC
BanII 640 GAGCCC G[GA]GC[CT]^C
BbvCI 933 CCTCAGC CCTCAGC(-5/-2)
BcgI 113 CGAAAGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1202 GGATAC GTATCC(6/5)
BlpI 647 GCTCAGC GC^T[ACGT]AGC
BmyI 640 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 647 GCTCAGC GC^T[ACGT]AGC
BsaAI 1645 TACGTG [CT]AC^GT[GA]
BsmI 574 GCATTC GAATGC(1/-1)
BsoBI 777 CCCGAG C^[CT]CG[GA]G
Bsp1286I 640 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 1611 TGTACA T^GTACA
BspLU11I 378 ACATGT A^CATGT
BsrBI 1366 GAGCGG CCGCTC(-3/-3)
BsrGI 1611 TGTACA T^GTACA
BssSI 1363 CACGAG CACGAG(-5/-1)
Bst1107I 866 GTATAC GTA^TAC
BstAPI 896 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 442 CCAGGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 866 GTATAC GTA^TAC
CelII 647 GCTCAGC GC^T[ACGT]AGC
DraI 832 TTTAAA TTT^AAA
DraII 781 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 610 CCTAGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 781 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 432 GAATTC G^AATTC
FauI 905 GCGGG CCCGC(4/6)
KpnI 177 GGTACC GGTAC^C
MaeII 1646 ACGT A^CGT
MseI 833 TTAA T^TAA
NspI 378 ACATGT [GA]CATG^[CT]
PflFI 233 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 781 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1482 CTGCAG CTGCA^G
SfaNI 270 GATGC GCATC(5/9)
SspBI 1611 TGTACA T^GTACA
TaqI 874 TCGA T^CGA
Tru9I 833 TTAA T^TAA
Tth111I 233 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 874 TCGA T^CGA
XmnI 950 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 905 GCGG CCGC(-3/-1)
1368 GCGG
AflIII 378 ACATGT A^C[GA][CT]GT
1646 ACGTGT
AvaII 744 GGACC G^G[AT]CC
782 GGACC
BglII 389 AGATCT A^GATCT
1266 AGATCT
BsaWI 741 ACCGGA [AT]^CCGG[AT]
1126 TCCGGT
BsmAI 79 GAGAC GTCTC(1/5)
231 GAGAC
BstYI 389 AGATCT [GA]^GATC[CT]
1266 AGATCT
Cac8I 119 GCTTGC GC[ACGT]^[ACGT]GC
905 GCGGGC
EaeI 1410 TGGCCG [CT]^GGCC[GA]
1662 TGGCCG
HgaI 1371 GACGC GACGC(5/10)
1539 GACGC
HphI 1129 GGTGA GGTGA(8/7)
1402 GGTGA
MflI 389 AGATCT [GA]^GATC[CT]
1266 AGATCT
MwoI 459 GCAGGGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
896 GCAGTGAGTGC
PleI 1035 GACTC GAGTC(4/5)
237 GAGTC
SfcI 726 CTGTAG C^T[GA][CT]AG
1482 CTGCAG
SmlI 567 CTCAAG C^T[CT][GA]AG
705 CTTGAG
XhoII 389 AGATCT [GA]^GATC[CT]
1266 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AluI BbvI BfaI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MslI MspI MvaI NdeII NlaIII
NlaIV PflMI RsaI Sau3AI Sau96I
ScrFI TfiI TseI Tsp45I Tsp509I
TspRI Van91I