Restriction map for
pF1KB6164
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AvaII AviII
AvrII BanI BbeI BbrPI BbsI
BcgI BclI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsiEI
BsiWI BsmI BspDI BspLU11I BspMI
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1165 GACGTC GACGT^C
AccI 1012 GTCTAC GT^[AC][GT]AC
AccIII 539 TCCGGA T^CCGGA
AcsI 418 AAATTC [GA]^AATT[CT]
AcyI 1165 GACGTC G[GA]^CG[CT]C
AflIII 1733 ACGTGT A^C[GA][CT]GT
AhdI 388 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 505 CCTGAGG CC^T[ACGT]AGG
ApaI 229 GGGCCC GGGCC^C
ApoI 418 AAATTC [GA]^AATT[CT]
Asp700I 531 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 388 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 750 GGATCC G^GATCC
BciVI 1554 GTATCC GTATCC(6/5)
BsaHI 1165 GACGTC G[GA]^CG[CT]C
BsaI 1609 GAGACC GGTCTC(1/5)
BsaWI 539 TCCGGA [AT]^CCGG[AT]
BseAI 539 TCCGGA T^CCGGA
BsmBI 868 GAGACG CGTCTC(1/5)
Bsp1407I 1736 TGTACA T^GTACA
BspEI 539 TCCGGA T^CCGGA
BspHI 1292 TCATGA T^CATGA
BsrGI 1736 TGTACA T^GTACA
BssSI 634 CTCGTG CACGAG(-5/-1)
BstAPI 1255 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 505 CCTGAGG CC^T[ACGT]AGG
CfoI 1759 GCGC GCG^C
DraIII 404 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1386 CCATGG C^C[GA][CT]GG
Eam1105I 388 GACTTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 505 CCTGAGG CC^T[ACGT]AGG
HhaI 1759 GCGC GCG^C
Hin1I 1165 GACGTC G[GA]^CG[CT]C
HinP1I 1759 GCGC G^CGC
HindIII 304 AAGCTT A^AGCTT
MroI 539 TCCGGA T^CCGGA
MslI 472 CATAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1386 CCATGG C^CATGG
PaeR7I 755 CTCGAG C^TCGAG
PstI 298 CTGCAG CTGCA^G
RcaI 1292 TCATGA T^CATGA
SacI 895 GAGCTC GAGCT^C
SapI 949 GAAGAGC GCTCTTC(1/4)
ScaI 218 AGTACT AGT^ACT
SspBI 1736 TGTACA T^GTACA
XcmI 1742 CCATCAAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 755 CTCGAG C^TCGAG
XmnI 531 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BalI 619 TGGCCA TGG^CCA
1220 TGGCCA
BcnI 816 CCGGG CC^[GC]GG
1538 CCCGG
BlpI 204 GCTCAGC GC^T[ACGT]AGC
892 GCTGAGC
Bpu1102I 204 GCTCAGC GC^T[ACGT]AGC
892 GCTGAGC
BsgI 1343 CTGCAC GTGCAG(16/14)
1449 CTGCAC
BsrBI 337 GAGCGG CCGCTC(-3/-3)
760 GAGCGG
BsrFI 1077 GCCGGT [GA]^CCGG[CT]
1484 GCCGGT
BstYI 750 GGATCC [GA]^GATC[CT]
905 AGATCC
CelII 204 GCTCAGC GC^T[ACGT]AGC
892 GCTGAGC
Cfr10I 1077 GCCGGT [GA]^CCGG[CT]
1484 GCCGGT
DraII 228 GGGGCCC [GA]G^G[ACGT]CC[CT]
229 GGGCCCC
EaeI 619 TGGCCA [CT]^GGCC[GA]
1220 TGGCCA
EcoNI 987 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1050 CCTGGAGAAGG
EcoO109I 228 GGGGCCC [GA]G^G[ACGT]CC[CT]
229 GGGCCCC
HgaI 543 GACGC GACGC(5/10)
171 GCGTC
MflI 750 GGATCC [GA]^GATC[CT]
905 AGATCC
MluNI 619 TGGCCA TGG^CCA
1220 TGGCCA
MscI 619 TGGCCA TGG^CCA
1220 TGGCCA
MseI 944 TTAA T^TAA
1544 TTAA
NciI 816 CCGGG CC^[GC]GG
1538 CCCGG
Tru9I 944 TTAA T^TAA
1544 TTAA
XhoII 750 GGATCC [GA]^GATC[CT]
905 AGATCC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI BanII BbvCI BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I