Restriction map for
pF1KB6830
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Alw44I 137 GTGCAC G^TGCAC
AlwNI 283 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 409 CCTGAGG CC^T[ACGT]AGG
ApaLI 137 GTGCAC G^TGCAC
AspHI 137 GTGCAC G[AT]GC[AT]^C
AvaI 530 CCCGGG C^[CT]CG[GA]G
BamHI 275 GGATCC G^GATCC
BbvCI 390 CCTCAGC CCTCAGC(-5/-2)
BmyI 137 GTGCAC G[AGT]GC[ACT]^C
BsgI 120 CTGCAC GTGCAG(16/14)
BsiHKAI 137 GTGCAC G[AT]GC[AT]^C
BsiYI 145 CCACTAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 145 CCACTAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 472 CGTCTC CGTCTC(1/5)
BsoBI 530 CCCGGG C^[CT]CG[GA]G
Bsp1286I 137 GTGCAC G[AGT]GC[ACT]^C
BstEII 415 GGTCACC G^GT[ACGT]ACC
BstF5I 142 CATCC GGATG(2/0)
BstPI 415 GGTCACC G^GT[ACGT]ACC
Bsu36I 409 CCTGAGG CC^T[ACGT]AGG
Eco81I 409 CCTGAGG CC^T[ACGT]AGG
EcoO65I 415 GGTCACC G^GT[ACGT]ACC
FokI 142 CATCC GGATG(9/13)
HapII 531 CCGG C^CGG
HgaI 471 GCGTC GACGC(5/10)
HpaII 531 CCGG C^CGG
HphI 417 TCACC GGTGA(8/7)
MspI 531 CCGG C^CGG
PshAI 250 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 403 CTGCAG CTGCA^G
SmaI 530 CCCGGG CCC^GGG
SpeI 147 ACTAGT A^CTAGT
StuI 509 AGGCCT AGG^CCT
TfiI 438 GATTC G^A[AT]TC
XmaCI 530 CCCGGG C^CCGGG
XmaI 530 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 483 CCGC CCGC(-3/-1)
527 CCGC
BcnI 530 CCCGG CC^[GC]GG
531 CCGGG
BfaI 148 CTAG C^TAG
310 CTAG
BglI 356 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
511 GCCTTTGTGGC
BpmI 505 CTCCAG CTGGAG(16/14)
132 CTGGAG
BstYI 162 GGATCT [GA]^GATC[CT]
275 GGATCC
Cfr13I 363 GGGCC G^G[ACGT]CC
387 GGGCC
DsaI 463 CCATGG C^C[GA][CT]GG
543 CCATGG
EarI 172 GAAGAG CTCTTC(1/4)
184 GAAGAG
Ksp632I 172 GAAGAG CTCTTC(1/4)
184 GAAGAG
MaeI 148 CTAG C^TAG
310 CTAG
MaeIII 230 GTGAC ^GT[ACGT]AC
416 GTCAC
MboII 172 GAAGA GAAGA(8/7)
184 GAAGA
MflI 162 GGATCT [GA]^GATC[CT]
275 GGATCC
NciI 530 CCCGG CC^[GC]GG
531 CCGGG
NcoI 463 CCATGG C^CATGG
543 CCATGG
NlaIV 275 GGATCC GG[ACGT]^[ACGT]CC
333 GGAGCC
Sau96I 363 GGGCC G^G[ACGT]CC
387 GGGCC
SfcI 403 CTGCAG C^T[GA][CT]AG
521 CTGTAG
Tsp45I 230 GTGAC ^GT[GC]AC
416 GTCAC
XcmI 207 CCATATCCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
213 CCAGTGTGGAGATGG
XhoII 162 GGATCT [GA]^GATC[CT]
275 GGATCC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsmAI
BsmFI BsrI BssKI BstNI Cac8I
DdeI DpnII EcoRII EcoT14I Fnu4HI
HaeIII HinfI ItaI MboI MnlI
MvaI MwoI NdeII NlaIII PleI
Sau3AI ScrFI StyI TseI TspRI