Restriction map for
pF1KB6173
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
BbeI BbsI BbvCI BclI BfrI
BglI BglII BpuAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspDI BspHI
BspLU11I BsrBI BsrFI BssSI Bst1107I
BstBI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FauI FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SfiI SfoI SfuI SgrAI
SnaBI SpeI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AflIII 1392 ACGTGT A^C[GA][CT]GT
Alw44I 632 GTGCAC G^TGCAC
ApaI 912 GGGCCC GGGCC^C
ApaLI 632 GTGCAC G^TGCAC
AviII 1349 TGCGCA TGC^GCA
AvrII 627 CCTAGG C^CTAGG
BalI 217 TGGCCA TGG^CCA
BamHI 288 GGATCC G^GATCC
BbrPI 1391 CACGTG CAC^GTG
BcgI 783 CGAGGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 258 GTATCC GTATCC(6/5)
BlnI 627 CCTAGG C^CTAGG
BsaAI 1391 CACGTG [CT]AC^GT[GA]
BsgI 416 GTGCAG GTGCAG(16/14)
BsmI 372 GAATGC GAATGC(1/-1)
Bsp1407I 113 TGTACA T^GTACA
BsrDI 1442 GCAATG GCAATG(2/0)
BsrGI 113 TGTACA T^GTACA
BssHII 478 GCGCGC G^CGCGC
BstAPI 767 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 463 GGTGACC G^GT[ACGT]ACC
BstPI 463 GGTGACC G^GT[ACGT]ACC
EaeI 217 TGGCCA [CT]^GGCC[GA]
EcoNI 702 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 463 GGTGACC G^GT[ACGT]ACC
EcoT22I 1856 ATGCAT ATGCA^T
FspI 1349 TGCGCA TGC^GCA
MluNI 217 TGGCCA TGG^CCA
MscI 217 TGGCCA TGG^CCA
MseI 1370 TTAA T^TAA
MspA1I 271 CAGCTG C[AC]G^C[GT]G
NdeI 1853 CATATG CA^TATG
NsiI 1856 ATGCAT ATGCA^T
NspI 1858 GCATGC [GA]CATG^[CT]
PaeR7I 781 CTCGAG C^TCGAG
PmaCI 1391 CACGTG CAC^GTG
PmlI 1391 CACGTG CAC^GTG
PstI 746 CTGCAG CTGCA^G
PvuII 271 CAGCTG CAG^CTG
SbfI 745 CCTGCAGG CCTGCA^GG
SmaI 524 CCCGGG CCC^GGG
SphI 1858 GCATGC GCATG^C
Sse8387I 745 CCTGCAGG CCTGCA^GG
SspBI 113 TGTACA T^GTACA
SspI 1542 AATATT AAT^ATT
StuI 1877 AGGCCT AGG^CCT
TaqI 782 TCGA T^CGA
Tru9I 1370 TTAA T^TAA
TthHB8I 782 TCGA T^CGA
XhoI 781 CTCGAG C^TCGAG
XmaCI 524 CCCGGG C^CCGGG
XmaI 524 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccIII 650 TCCGGA T^CCGGA
1547 TCCGGA
AlwNI 595 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1738 CAGCACCTG
Asp700I 736 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1455 GAAAACCTTC
AvaI 524 CCCGGG C^[CT]CG[GA]G
781 CTCGAG
BfaI 628 CTAG C^TAG
1337 CTAG
BlpI 1724 GCTCAGC GC^T[ACGT]AGC
1762 GCTAAGC
Bpu1102I 1724 GCTCAGC GC^T[ACGT]AGC
1762 GCTAAGC
BseAI 650 TCCGGA T^CCGGA
1547 TCCGGA
BsoBI 524 CCCGGG C^[CT]CG[GA]G
781 CTCGAG
BspEI 650 TCCGGA T^CCGGA
1547 TCCGGA
CelII 1724 GCTCAGC GC^T[ACGT]AGC
1762 GCTAAGC
DrdI 18 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
717 GACCTGGATGTC
EcoRI 233 GAATTC G^AATTC
1496 GAATTC
MaeI 628 CTAG C^TAG
1337 CTAG
MroI 650 TCCGGA T^CCGGA
1547 TCCGGA
SapI 1410 GAAGAGC GCTCTTC(1/4)
1640 GCTCTTC
ScaI 738 AGTACT AGT^ACT
1471 AGTACT
SexAI 146 ACCTGGT A^CC[AT]GGT
965 ACCTGGT
TfiI 664 GAATC G^A[AT]TC
1066 GAATC
XmnI 736 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1455 GAAAACCTTC
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII