##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        Alw44I      AlwNI       Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TaqI        Tru9I       Tsp509I     
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AfaI          441  GTAC                 GT^AC
AhdI          128  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          403  CCTCAGG              CC^T[ACGT]AGG
AspEI         128  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         499  GAGCTC               G[AT]GC[AT]^C
BpmI          239  CTGGAG               CTGGAG(16/14)
BsiHKAI       499  GAGCTC               G[AT]GC[AT]^C
BsmAI         303  GTCTC                GTCTC(1/5)
BspMI         275  ACCTGC               ACCTGC(4/8)
BstF5I        220  CATCC                GGATG(2/0)
Bsu36I        403  CCTCAGG              CC^T[ACGT]AGG
Eam1105I      128  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          253  GAAGAG               CTCTTC(1/4)
Eco81I        403  CCTCAGG              CC^T[ACGT]AGG
FokI          220  CATCC                GGATG(9/13)
HphI          369  GGTGA                GGTGA(8/7)
Ksp632I       253  GAAGAG               CTCTTC(1/4)
MaeIII        151  GTGAC                ^GT[ACGT]AC
MboII         253  GAAGA                GAAGA(8/7)
NlaIII         60  CATG                 CATG^
PflMI         334  CCAACTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         296  GACGGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         391  CAGCTG               CAG^CTG
RsaI          441  GTAC                 GT^AC
SacI          499  GAGCTC               GAGCT^C
ScaI          440  AGTACT               AGT^ACT
SmaI          536  CCCGGG               CCC^GGG
SmlI          305  CTCAAG               C^T[CT][GA]AG
TfiI          101  GAATC                G^A[AT]TC
Tsp45I        151  GTGAC                ^GT[GC]AC
Van91I        334  CCAACTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         536  CCCGGG               C^CCGGG
XmaI          536  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI          392  AGCT                 AG^CT
              500  AGCT                
ApaI          486  GGGCCC               GGGCC^C
              533  GGGCCC              
BamHI         330  GGATCC               G^GATCC
              413  GGATCC              
BseRI         401  CTCCTC               GAGGAG(10/8)
              364  GAGGAG              
BsgI           78  CTGCAC               GTGCAG(16/14)
              179  CTGCAC              
BsrI           96  ACTGG                ACTGG(1/-1)
              192  CCAGT               
FauI          261  CCCGC                CCCGC(4/6)
              527  CCCGC               
HgaI          153  GACGC                GACGC(5/10)
              233  GACGC               
MspA1I        262  CCGCTG               C[AC]G^C[GT]G
              391  CAGCTG              
PpuMI         134  GGGTCCT              [GA]G^G[AT]CC[CT]
              257  AGGTCCC             


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AlwI        AvaI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BssKI       
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      Fnu4HI      
HaeIII      HapII       HinfI       HpaII       ItaI        
MboI        MflI        MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIV       PleI        
PstI        Sau3AI      Sau96I      ScrFI       SfcI        
StuI        TseI        TspRI       XhoII