Restriction map for
pF1KB6190
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbsI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Alw44I 348 GTGCAC G^TGCAC
AlwNI 340 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 348 GTGCAC G^TGCAC
AseI 799 ATTAAT AT^TAAT
AsnI 799 ATTAAT AT^TAAT
AvaI 71 CTCGGG C^[CT]CG[GA]G
AvaII 21 GGACC G^G[AT]CC
BanI 186 GGCACC G^G[CT][GA]CC
BanII 1922 GAGCTC G[GA]GC[CT]^C
BbrPI 353 CACGTG CAC^GTG
BbvCI 736 GCTGAGG CCTCAGC(-5/-2)
BglII 997 AGATCT A^GATCT
BsaI 744 GAGACC GGTCTC(1/5)
BsoBI 71 CTCGGG C^[CT]CG[GA]G
BspLU11I 482 ACATGT A^CATGT
BsrDI 1598 GCAATG GCAATG(2/0)
BssSI 1927 CACGAG CACGAG(-5/-1)
DrdI 234 GACTGCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 90 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 639 CCAAGG C^C[AT][AT]GG
MspA1I 5 CAGCGG C[AC]G^C[GT]G
PmaCI 353 CACGTG CAC^GTG
PmlI 353 CACGTG CAC^GTG
PshBI 799 ATTAAT AT^TAAT
PstI 1008 CTGCAG CTGCA^G
SacI 1922 GAGCTC GAGCT^C
SapI 465 GAAGAGC GCTCTTC(1/4)
SmlI 91 CTTGAG C^T[CT][GA]AG
SnaBI 1843 TACGTA TAC^GTA
StyI 639 CCAAGG C^C[AT][AT]GG
TaqI 47 TCGA T^CGA
TthHB8I 47 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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BcnI 24 CCGGG CC^[GC]GG
1241 CCGGG
BfaI 157 CTAG C^TAG
1295 CTAG
BseRI 76 GAGGAG GAGGAG(10/8)
94 GAGGAG
BsmI 595 GAATGC GAATGC(1/-1)
1258 GAATGC
Bsp1407I 472 TGTACA T^GTACA
1557 TGTACA
BsrGI 472 TGTACA T^GTACA
1557 TGTACA
DraII 451 GGGGCCT [GA]G^G[ACGT]CC[CT]
1249 AGGCCCT
EarI 710 CTCTTC CTCTTC(1/4)
465 GAAGAG
EcoO109I 451 GGGGCCT [GA]G^G[ACGT]CC[CT]
1249 AGGCCCT
EcoT22I 597 ATGCAT ATGCA^T
1260 ATGCAT
HapII 24 CCGG C^CGG
1241 CCGG
HpaII 24 CCGG C^CGG
1241 CCGG
Ksp632I 710 CTCTTC CTCTTC(1/4)
465 GAAGAG
MaeI 157 CTAG C^TAG
1295 CTAG
MspI 24 CCGG C^CGG
1241 CCGG
NciI 24 CCGGG CC^[GC]GG
1241 CCGGG
NlaIV 186 GGCACC GG[ACGT]^[ACGT]CC
451 GGGGCC
NsiI 597 ATGCAT ATGCA^T
1260 ATGCAT
NspI 482 ACATGT [GA]CATG^[CT]
922 ACATGC
SspBI 472 TGTACA T^GTACA
1557 TGTACA
TfiI 107 GATTC G^A[AT]TC
374 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI BbvI
BmyI BpmI BsaAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI