Restriction map for
pF1KB6894
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# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 372 GATCC GGATC(4/5)
AvaII 363 GGACC G^G[AT]CC
BanI 337 GGCACC G^G[CT][GA]CC
BanII 66 GAGCCC G[GA]GC[CT]^C
BbrPI 478 CACGTG CAC^GTG
BbsI 529 GTCTTC GAAGAC(2/6)
BbvCI 390 CCTCAGC CCTCAGC(-5/-2)
BbvI 467 GCAGC GCAGC(8/12)
BglI 586 GCCGTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 529 GTCTTC GAAGAC(2/6)
BsaAI 478 CACGTG [CT]AC^GT[GA]
BsiYI 30 CCTGCTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 30 CCTGCTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 362 GGGAC GGGAC(10/14)
BspHI 131 TCATGA T^CATGA
BstYI 371 AGATCC [GA]^GATC[CT]
Cac8I 404 GCAAGC GC[ACGT]^[ACGT]GC
EcoT14I 333 CCTTGG C^C[AT][AT]GG
HapII 360 CCGG C^CGG
HpaII 360 CCGG C^CGG
MflI 371 AGATCC [GA]^GATC[CT]
MspI 360 CCGG C^CGG
PmaCI 478 CACGTG CAC^GTG
PmlI 478 CACGTG CAC^GTG
RcaI 131 TCATGA T^CATGA
SmaI 359 CCCGGG CCC^GGG
StyI 333 CCTTGG C^C[AT][AT]GG
TseI 467 GCAGC G^C[AT]GC
XhoII 371 AGATCC [GA]^GATC[CT]
XmaCI 359 CCCGGG C^CCGGG
XmaI 359 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 359 CCCGGG C^[CT]CG[GA]G
516 CTCGGG
BcnI 359 CCCGG CC^[GC]GG
360 CCGGG
BsgI 43 CTGCAC GTGCAG(16/14)
465 GTGCAG
BsoBI 359 CCCGGG C^[CT]CG[GA]G
516 CTCGGG
BstF5I 426 CATCC GGATG(2/0)
525 GGATG
BstXI 224 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
299 CCAAGCTGGTGG
DdeI 391 CTCAG C^T[ACGT]AG
442 CTTAG
FauI 267 CCCGC CCCGC(4/6)
61 GCGGG
FokI 426 CATCC GGATG(9/13)
525 GGATG
HincII 88 GTCAAC GT[CT]^[GA]AC
554 GTCAAC
HindII 88 GTCAAC GT[CT]^[GA]AC
554 GTCAAC
HphI 80 TCACC GGTGA(8/7)
356 TCACC
MslI 321 CATAGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
582 CATCGCCGTG
NciI 359 CCCGG CC^[GC]GG
360 CCGGG
StuI 435 AGGCCT AGG^CCT
599 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AfaI AluI BmyI BsaJI
Bsp1286I BsrI BssKI BstNI Cfr13I
DpnII EcoRII Fnu4HI HaeIII ItaI
MaeII MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI Tsp45I
TspRI