Restriction map for
pF1KB6848
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraII DraIII DsaI Eam1105I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FbaI FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccIII 275 TCCGGA T^CCGGA
Aor51HI 304 AGCGCT AGC^GCT
AspHI 17 GAGCTC G[AT]GC[AT]^C
AvaI 29 CCCGGG C^[CT]CG[GA]G
BalI 476 TGGCCA TGG^CCA
BbvCI 53 CCTCAGC CCTCAGC(-5/-2)
BbvI 119 GCAGC GCAGC(8/12)
BcgI 260 CGAAGACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 184 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 275 TCCGGA [AT]^CCGG[AT]
BseAI 275 TCCGGA T^CCGGA
BsiEI 256 CGGCCG CG[GA][CT]^CG
BsiHKAI 17 GAGCTC G[AT]GC[AT]^C
BsmAI 279 GAGAC GTCTC(1/5)
BsmFI 26 GTCCC GGGAC(10/14)
BsoBI 29 CCCGGG C^[CT]CG[GA]G
BspEI 275 TCCGGA T^CCGGA
BstNI 311 CCAGG CC^[AT]GG
BstYI 230 AGATCC [GA]^GATC[CT]
CfoI 305 GCGC GCG^C
Cfr13I 32 GGGCC G^G[ACGT]CC
DraI 177 TTTAAA TTT^AAA
DrdI 357 GACCACTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 256 CGGCCG C^GGCCG
EarI 300 GAAGAG CTCTTC(1/4)
EclXI 256 CGGCCG C^GGCCG
Eco47III 304 AGCGCT AGC^GCT
Eco52I 256 CGGCCG C^GGCCG
EcoRII 311 CCAGG ^CC[AT]GG
EcoT14I 479 CCAAGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
FauI 104 CCCGC CCCGC(4/6)
HaeII 304 AGCGCT [GA]GCGC^[CT]
HhaI 305 GCGC GCG^C
HinP1I 305 GCGC G^CGC
HincII 484 GTCAAC GT[CT]^[GA]AC
HindII 484 GTCAAC GT[CT]^[GA]AC
HindIII 322 AAGCTT A^AGCTT
HphI 241 TCACC GGTGA(8/7)
Ksp632I 300 GAAGAG CTCTTC(1/4)
MaeII 238 ACGT A^CGT
MaeIII 240 GTCAC ^GT[ACGT]AC
MflI 230 AGATCC [GA]^GATC[CT]
MluNI 476 TGGCCA TGG^CCA
MroI 275 TCCGGA T^CCGGA
MscI 476 TGGCCA TGG^CCA
MspA1I 352 CCGCTG C[AC]G^C[GT]G
MvaI 311 CCAGG CC^[AT]GG
NdeI 521 CATATG CA^TATG
NsiI 1 ATGCAT ATGCA^T
PflMI 7 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 17 GAGCTC GAGCT^C
SapI 300 GAAGAGC GCTCTTC(1/4)
Sau96I 32 GGGCC G^G[ACGT]CC
SmaI 29 CCCGGG CCC^GGG
StyI 479 CCAAGG C^C[AT][AT]GG
TaqI 528 TCGA T^CGA
TfiI 495 GATTC G^A[AT]TC
TseI 119 GCAGC G^C[AT]GC
Tsp45I 240 GTCAC ^GT[GC]AC
TthHB8I 528 TCGA T^CGA
Van91I 7 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 65 CCATTTTCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 230 AGATCC [GA]^GATC[CT]
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 231 GATCC GGATC(4/5)
575 GGATC
BanII 17 GAGCTC G[GA]GC[CT]^C
137 GGGCTC
BbsI 261 GAAGAC GAAGAC(2/6)
385 GAAGAC
BpuAI 261 GAAGAC GAAGAC(2/6)
385 GAAGAC
BsrI 9 ACTGG ACTGG(1/-1)
531 ACTGG
DdeI 54 CTCAG C^T[ACGT]AG
362 CTGAG
DpnII 231 GATC ^GATC
576 GATC
EaeI 256 CGGCCG [CT]^GGCC[GA]
476 TGGCCA
Fnu4HI 119 GCAGC GC^[ACGT]GC
255 GCGGC
ItaI 119 GCAGC GC^[ACGT]GC
255 GCGGC
MboI 231 GATC ^GATC
576 GATC
MslI 396 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
468 CATTGATGTG
MwoI 175 GCTTTAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
184 GCCAAGAAGGC
NdeII 231 GATC ^GATC
576 GATC
NlaIV 138 GGCTCC GG[ACGT]^[ACGT]CC
534 GGCTCC
PleI 272 GACTC GAGTC(4/5)
364 GAGTC
Sau3AI 231 GATC ^GATC
576 GATC
SfaNI 547 GATGC GCATC(5/9)
3 GCATC
Tsp509I 44 AATT ^AATT
570 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
FokI HaeIII HapII HinfI HpaII
MboII MnlI MseI MspI NciI
NlaIII ScrFI Tru9I TspRI