Restriction map for
pF1KB6219
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BbrPI BbsI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MaeI MaeII MamI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SapI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI TfiI
Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 27 GTCGAC GT^[AC][GT]AC
Alw44I 1375 GTGCAC G^TGCAC
ApaLI 1375 GTGCAC G^TGCAC
Asp700I 1801 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1304 TGGCCA TGG^CCA
BamHI 1160 GGATCC G^GATCC
BbeI 1099 GGCGCC GGCGC^C
BsaWI 1039 ACCGGA [AT]^CCGG[AT]
BseRI 415 GAGGAG GAGGAG(10/8)
BsgI 1174 CTGCAC GTGCAG(16/14)
BsrBI 481 GAGCGG CCGCTC(-3/-3)
BsrFI 74 GCCGGC [GA]^CCGG[CT]
BssHII 18 GCGCGC G^CGCGC
BstYI 1160 GGATCC [GA]^GATC[CT]
Cfr10I 74 GCCGGC [GA]^CCGG[CT]
HincII 27 GTCGAC GT[CT]^[GA]AC
HindII 27 GTCGAC GT[CT]^[GA]AC
KasI 1099 GGCGCC G^GCGCC
MflI 1160 GGATCC [GA]^GATC[CT]
MluNI 1304 TGGCCA TGG^CCA
MscI 1304 TGGCCA TGG^CCA
MseI 1018 TTAA T^TAA
NaeI 74 GCCGGC GCC^GGC
NarI 1099 GGCGCC GG^CGCC
NdeI 580 CATATG CA^TATG
NgoMIV 74 GCCGGC G^CCGGC
NspI 194 GCATGC [GA]CATG^[CT]
PshAI 1237 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 27 GTCGAC G^TCGAC
SbfI 1866 CCTGCAGG CCTGCA^GG
SexAI 227 ACCAGGT A^CC[AT]GGT
SfoI 1099 GGCGCC GGC^GCC
SphI 194 GCATGC GCATG^C
Sse8387I 1866 CCTGCAGG CCTGCA^GG
StuI 158 AGGCCT AGG^CCT
TaqI 28 TCGA T^CGA
Tru9I 1018 TTAA T^TAA
TthHB8I 28 TCGA T^CGA
XhoII 1160 GGATCC [GA]^GATC[CT]
XmnI 1801 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 1161 GATCC GGATC(4/5)
1160 GGATC
AlwNI 445 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
649 CAGTGCCTG
AspI 447 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
850 GACGCGGTC
AvaI 56 CCCGGG C^[CT]CG[GA]G
1963 CCCGGG
BbvCI 723 CCTCAGC CCTCAGC(-5/-2)
1676 CCTCAGC
BcgI 765 CGACAGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1071 GCAACACTCTCG
BsoBI 56 CCCGGG C^[CT]CG[GA]G
1963 CCCGGG
BspMI 188 ACCTGC ACCTGC(4/8)
600 ACCTGC
KspI 132 CCGCGG CCGC^GG
522 CCGCGG
PflFI 447 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
850 GACGCGGTC
PflMI 732 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
834 CCACGCGCTGG
SacI 367 GAGCTC GAGCT^C
1725 GAGCTC
SacII 132 CCGCGG CCGC^GG
522 CCGCGG
SmaI 56 CCCGGG CCC^GGG
1963 CCCGGG
SmlI 541 CTCAAG C^T[CT][GA]AG
1603 CTTGAG
Tth111I 447 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
850 GACGCGGTC
Van91I 732 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
834 CCACGCGCTGG
XcmI 455 CCAGGAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1292 CCAGCACCCCGCTGG
XmaCI 56 CCCGGG C^CCGGG
1963 CCCGGG
XmaI 56 CCCGGG C^CCGGG
1963 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaII AviII BanI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI