Restriction map for
pF1KB6906
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 522 CGCG CG^CG
AciI 521 CCGC CCGC(-3/-1)
AspHI 297 GTGCTC G[AT]GC[AT]^C
BanI 455 GGTGCC G^G[CT][GA]CC
BbsI 313 GTCTTC GAAGAC(2/6)
BpuAI 313 GTCTTC GAAGAC(2/6)
BsaI 417 GGTCTC GGTCTC(1/5)
BsgI 585 CTGCAC GTGCAG(16/14)
BsiHKAI 297 GTGCTC G[AT]GC[AT]^C
BsiYI 651 CCACACCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 651 CCACACCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 418 GTCTC GTCTC(1/5)
BsmFI 529 GTCCC GGGAC(10/14)
Bsp1407I 173 TGTACA T^GTACA
BsrGI 173 TGTACA T^GTACA
BstUI 522 CGCG CG^CG
BstYI 109 GGATCT [GA]^GATC[CT]
DrdI 3 GACCCGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 180 CGGCCA [CT]^GGCC[GA]
EcoT14I 151 CCTTGG C^C[AT][AT]GG
EcoT22I 73 ATGCAT ATGCA^T
HaeIII 181 GGCC GG^CC
HinfI 58 GAGTC G^A[ACGT]TC
MflI 109 GGATCT [GA]^GATC[CT]
MseI 581 TTAA T^TAA
MslI 654 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 258 CAGCTG C[AC]G^C[GT]G
MvnI 522 CGCG CG^CG
NsiI 73 ATGCAT ATGCA^T
PleI 58 GAGTC GAGTC(4/5)
PvuII 258 CAGCTG CAG^CTG
ScaI 191 AGTACT AGT^ACT
SfcI 402 CTACAG C^T[GA][CT]AG
SspBI 173 TGTACA T^GTACA
StyI 151 CCTTGG C^C[AT][AT]GG
Tru9I 581 TTAA T^TAA
XhoII 109 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 174 GTAC GT^AC
192 GTAC
AlwI 109 GGATC GGATC(4/5)
598 GGATC
AvaII 11 GGTCC G^G[AT]CC
528 GGTCC
BbvI 275 GCAGC GCAGC(8/12)
260 GCTGC
BmyI 297 GTGCTC G[AGT]GC[ACT]^C
456 GTGCCC
Bsp1286I 297 GTGCTC G[AGT]GC[ACT]^C
456 GTGCCC
BsrI 14 CCAGT ACTGG(1/-1)
183 CCAGT
Cfr13I 11 GGTCC G^G[ACGT]CC
528 GGTCC
DpnII 110 GATC ^GATC
599 GATC
Fnu4HI 260 GCTGC GC^[ACGT]GC
275 GCAGC
HphI 98 TCACC GGTGA(8/7)
305 TCACC
ItaI 260 GCTGC GC^[ACGT]GC
275 GCAGC
MboI 110 GATC ^GATC
599 GATC
MwoI 160 GCCACCAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
278 GCTTCTTAGGC
NdeII 110 GATC ^GATC
599 GATC
NlaIV 455 GGTGCC GG[ACGT]^[ACGT]CC
528 GGTCCC
RsaI 174 GTAC GT^AC
192 GTAC
Sau3AI 110 GATC ^GATC
599 GATC
Sau96I 11 GGTCC G^G[ACGT]CC
528 GGTCC
TaqI 240 TCGA T^CGA
317 TCGA
TseI 260 GCTGC G^C[AT]GC
275 GCAGC
Tsp45I 304 GTCAC ^GT[GC]AC
545 GTCAC
TspRI 184 CAGTG CA[GC]TG[ACGT][ACGT]^
331 CAGTG
TthHB8I 240 TCGA T^CGA
317 TCGA
XcmI 14 CCAGTTACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
464 CCACAGAGAACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BssKI
BstF5I BstNI DdeI EcoRII FokI
MaeIII MboII MnlI MvaI NlaIII
ScrFI SfaNI Tsp509I