Restriction map for
pF1KB6225
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AflII AflIII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I AspEI BamHI BciVI
BclI BfrI BglII BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
HindIII HpaI KspI MamI MluI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfuI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 437 GGTACC G^GTACC
AccIII 113 TCCGGA T^CCGGA
AclI 1983 AACGTT AA^CGTT
AcsI 1975 AAATTC [GA]^AATT[CT]
Aor51HI 347 AGCGCT AGC^GCT
ApoI 1975 AAATTC [GA]^AATT[CT]
Asp718I 437 GGTACC G^GTACC
AviII 1646 TGCGCA TGC^GCA
AvrII 1050 CCTAGG C^CTAGG
BbrPI 315 CACGTG CAC^GTG
BcgI 1530 GCAGGTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1050 CCTAGG C^CTAGG
BseAI 113 TCCGGA T^CCGGA
BsgI 1528 GTGCAG GTGCAG(16/14)
BsmBI 1586 CGTCTC CGTCTC(1/5)
BsmI 643 GCATTC GAATGC(1/-1)
BspEI 113 TCCGGA T^CCGGA
BssSI 1538 CTCGTG CACGAG(-5/-1)
DrdI 711 GACGGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 347 AGCGCT AGC^GCT
FseI 945 GGCCGGCC GGCCGG^CC
FspI 1646 TGCGCA TGC^GCA
HincII 418 GTTGAC GT[CT]^[GA]AC
HindII 418 GTTGAC GT[CT]^[GA]AC
KpnI 437 GGTACC GGTAC^C
MfeI 875 CAATTG C^AATTG
MroI 113 TCCGGA T^CCGGA
MseI 137 TTAA T^TAA
MslI 810 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 875 CAATTG C^AATTG
PaeR7I 1778 CTCGAG C^TCGAG
PmaCI 315 CACGTG CAC^GTG
PmlI 315 CACGTG CAC^GTG
PshAI 887 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1983 AACGTT AA^CGTT
SexAI 1352 ACCAGGT A^CC[AT]GGT
SfiI 945 GGCCGGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 908 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 89 CCCGGG CCC^GGG
SphI 392 GCATGC GCATG^C
Tru9I 137 TTAA T^TAA
XhoI 1778 CTCGAG C^TCGAG
XmaCI 89 CCCGGG C^CCGGG
XmaI 89 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
BalI 57 TGGCCA TGG^CCA
241 TGGCCA
BbeI 912 GGCGCC GGCGC^C
1499 GGCGCC
BbsI 1882 GAAGAC GAAGAC(2/6)
81 GTCTTC
BbvCI 956 CCTCAGC CCTCAGC(-5/-2)
1637 CCTCAGC
BglI 946 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1271 GCCGCCTGGGC
BpuAI 1882 GAAGAC GAAGAC(2/6)
81 GTCTTC
BsaAI 315 CACGTG [CT]AC^GT[GA]
1525 TACGTG
BseRI 1812 CTCCTC GAGGAG(10/8)
502 GAGGAG
BsiEI 944 CGGCCG CG[GA][CT]^CG
1269 CGGCCG
BsrBI 1611 CCGCTC CCGCTC(-3/-3)
387 GAGCGG
BsrDI 399 CATTGC GCAATG(2/0)
176 GCAATG
BsrFI 909 GCCGGC [GA]^CCGG[CT]
946 GCCGGC
BstXI 1664 CCACCGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1740 CCAGAATCTTGG
BstYI 278 AGATCC [GA]^GATC[CT]
1322 AGATCC
Cfr10I 909 GCCGGC [GA]^CCGG[CT]
946 GCCGGC
DsaI 377 CCGTGG C^C[GA][CT]GG
1266 CCACGG
EagI 944 CGGCCG C^GGCCG
1269 CGGCCG
EclXI 944 CGGCCG C^GGCCG
1269 CGGCCG
Eco52I 944 CGGCCG C^GGCCG
1269 CGGCCG
KasI 912 GGCGCC G^GCGCC
1499 GGCGCC
MflI 278 AGATCC [GA]^GATC[CT]
1322 AGATCC
MluNI 57 TGGCCA TGG^CCA
241 TGGCCA
MscI 57 TGGCCA TGG^CCA
241 TGGCCA
NaeI 909 GCCGGC GCC^GGC
946 GCCGGC
NarI 912 GGCGCC GG^CGCC
1499 GGCGCC
NgoMIV 909 GCCGGC G^CCGGC
946 GCCGGC
NspI 392 GCATGC [GA]CATG^[CT]
981 ACATGC
PvuII 484 CAGCTG CAG^CTG
1655 CAGCTG
SacI 903 GAGCTC GAGCT^C
966 GAGCTC
SfoI 912 GGCGCC GGC^GCC
1499 GGCGCC
SmlI 1159 CTCAAG C^T[CT][GA]AG
1778 CTCGAG
TaqI 500 TCGA T^CGA
1779 TCGA
TthHB8I 500 TCGA T^CGA
1779 TCGA
XcmI 764 CCAAGAAACCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1787 CCAGCCATCCCCTGG
XhoII 278 AGATCC [GA]^GATC[CT]
1322 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I Tsp509I TspRI Tth111I Van91I