Restriction map for
pF1KB6234
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BsmBI Bsp1407I BspHI
BsrGI BssHII Bst1107I BstAPI BstBI
BstYI BstZ17I CelII CpoI DraI
DraIII DrdI Eam1105I EcoRI FseI
FspI HgaI HpaI KpnI KspI
MamI MfeI MflI MluI MseI
MunI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 1429 CGCG CG^CG
AcsI 1384 AAATTT [GA]^AATT[CT]
AcyI 1492 GGCGCC G[GA]^CG[CT]C
AlwNI 1770 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1254 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1408 AGCGCT AGC^GCT
ApoI 1384 AAATTT [GA]^AATT[CT]
AvaI 942 CTCGGG C^[CT]CG[GA]G
BbeI 1492 GGCGCC GGCGC^C
BbsI 1840 GAAGAC GAAGAC(2/6)
BbvCI 460 GCTGAGG CCTCAGC(-5/-2)
BclI 320 TGATCA T^GATCA
BpuAI 1840 GAAGAC GAAGAC(2/6)
BsaAI 748 TACGTA [CT]AC^GT[GA]
BsaHI 1492 GGCGCC G[GA]^CG[CT]C
BsiEI 904 CGGCCG CG[GA][CT]^CG
BsmI 1905 GAATGC GAATGC(1/-1)
BsoBI 942 CTCGGG C^[CT]CG[GA]G
BspDI 1798 ATCGAT AT^CGAT
BsrDI 1019 GCAATG GCAATG(2/0)
BssSI 1515 CACGAG CACGAG(-5/-1)
BstUI 1429 CGCG CG^CG
Bsu36I 1254 CCTGAGG CC^T[ACGT]AGG
ClaI 1798 ATCGAT AT^CGAT
DsaI 1610 CCACGG C^C[GA][CT]GG
EagI 904 CGGCCG C^GGCCG
EarI 1447 CTCTTC CTCTTC(1/4)
EclXI 904 CGGCCG C^GGCCG
Eco47III 1408 AGCGCT AGC^GCT
Eco52I 904 CGGCCG C^GGCCG
Eco81I 1254 CCTGAGG CC^T[ACGT]AGG
EcoNI 620 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1615 GATATC GAT^ATC
EcoT14I 1728 CCAAGG C^C[AT][AT]GG
EcoT22I 1418 ATGCAT ATGCA^T
FbaI 320 TGATCA T^GATCA
Hin1I 1492 GGCGCC G[GA]^CG[CT]C
HindIII 772 AAGCTT A^AGCTT
KasI 1492 GGCGCC G^GCGCC
Ksp632I 1447 CTCTTC CTCTTC(1/4)
MaeII 749 ACGT A^CGT
MvnI 1429 CGCG CG^CG
NaeI 2021 GCCGGC GCC^GGC
NarI 1492 GGCGCC GG^CGCC
NgoMIV 2021 GCCGGC G^CCGGC
NsiI 1418 ATGCAT ATGCA^T
PflMI 546 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1333 GAGCTC GAGCT^C
ScaI 536 AGTACT AGT^ACT
SfoI 1492 GGCGCC GGC^GCC
SnaBI 748 TACGTA TAC^GTA
SpeI 849 ACTAGT A^CTAGT
StyI 1728 CCAAGG C^C[AT][AT]GG
Van91I 546 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 726 TCCGGA T^CCGGA
1389 TCCGGA
AfaI 537 GTAC GT^AC
909 GTAC
AflIII 383 ACATGT A^C[GA][CT]GT
1050 ACATGT
Alw44I 352 GTGCAC G^TGCAC
694 GTGCAC
ApaI 616 GGGCCC GGGCC^C
1172 GGGCCC
ApaLI 352 GTGCAC G^TGCAC
694 GTGCAC
BalI 543 TGGCCA TGG^CCA
1784 TGGCCA
BcnI 405 CCGGG CC^[GC]GG
1976 CCGGG
BseAI 726 TCCGGA T^CCGGA
1389 TCCGGA
BseRI 939 CTCCTC GAGGAG(10/8)
1468 GAGGAG
BspEI 726 TCCGGA T^CCGGA
1389 TCCGGA
BspLU11I 383 ACATGT A^CATGT
1050 ACATGT
BspMI 761 ACCTGC ACCTGC(4/8)
754 GCAGGT
BsrBI 49 GAGCGG CCGCTC(-3/-3)
70 GAGCGG
BsrFI 710 ACCGGC [GA]^CCGG[CT]
2021 GCCGGC
BstEII 757 GGTCACC G^GT[ACGT]ACC
1237 GGTGACC
BstPI 757 GGTCACC G^GT[ACGT]ACC
1237 GGTGACC
BstXI 381 CCACATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1706 CCACCAACCTGG
Cfr10I 710 ACCGGC [GA]^CCGG[CT]
2021 GCCGGC
EcoO65I 757 GGTCACC G^GT[ACGT]ACC
1237 GGTGACC
MluNI 543 TGGCCA TGG^CCA
1784 TGGCCA
MroI 726 TCCGGA T^CCGGA
1389 TCCGGA
MscI 543 TGGCCA TGG^CCA
1784 TGGCCA
NciI 405 CCGGG CC^[GC]GG
1976 CCGGG
PpuMI 221 GGGACCC [GA]G^G[AT]CC[CT]
1479 GGGACCT
PstI 127 CTGCAG CTGCA^G
1756 CTGCAG
PvuII 547 CAGCTG CAG^CTG
1439 CAGCTG
RsaI 537 GTAC GT^AC
909 GTAC
SexAI 1482 ACCTGGT A^CC[AT]GGT
1712 ACCTGGT
SfcI 127 CTGCAG C^T[GA][CT]AG
1756 CTGCAG
SmlI 1267 CTCAAG C^T[CT][GA]AG
1336 CTCAAG
TaqI 492 TCGA T^CGA
1799 TCGA
Tsp509I 1385 AATT ^AATT
1630 AATT
TthHB8I 492 TCGA T^CGA
1799 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BanII BbvI BfaI BglI
BmyI BpmI BsaI BsaJI BsaWI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI