Restriction map for
pF1KB6240
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BanI BbeI BcgI BciVI BclI
BfrI BglI BlnI BsaI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BsrBI BssHII BssSI BstBI BstXI
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRV
FauI FbaI FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 1515 CGCG CG^CG
AclI 1582 AACGTT AA^CGTT
AcyI 120 GACGCC G[GA]^CG[CT]C
ApaI 1939 GGGCCC GGGCC^C
AvaI 625 CCCGGG C^[CT]CG[GA]G
AviII 1356 TGCGCA TGC^GCA
BalI 928 TGGCCA TGG^CCA
BbrPI 1521 CACGTG CAC^GTG
BfaI 79 CTAG C^TAG
BlpI 360 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 360 GCTGAGC GC^T[ACGT]AGC
BsaAI 1521 CACGTG [CT]AC^GT[GA]
BsaBI 239 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 120 GACGCC G[GA]^CG[CT]C
BsiEI 125 CGGCCG CG[GA][CT]^CG
BsmBI 118 GAGACG CGTCTC(1/5)
BsmI 1530 GAATGC GAATGC(1/-1)
BsoBI 625 CCCGGG C^[CT]CG[GA]G
BspLU11I 769 ACATGT A^CATGT
BsrDI 1825 GCAATG GCAATG(2/0)
BsrFI 123 GCCGGC [GA]^CCGG[CT]
Bst1107I 1742 GTATAC GTA^TAC
BstEII 1651 GGTAACC G^GT[ACGT]ACC
BstPI 1651 GGTAACC G^GT[ACGT]ACC
BstUI 1515 CGCG CG^CG
BstZ17I 1742 GTATAC GTA^TAC
CelII 360 GCTGAGC GC^T[ACGT]AGC
Cfr10I 123 GCCGGC [GA]^CCGG[CT]
DraIII 1288 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 125 CGGCCG C^GGCCG
EclXI 125 CGGCCG C^GGCCG
Eco52I 125 CGGCCG C^GGCCG
EcoO65I 1651 GGTAACC G^GT[ACGT]ACC
EcoRI 1618 GAATTC G^AATTC
EcoT22I 1409 ATGCAT ATGCA^T
FspI 1356 TGCGCA TGC^GCA
HaeII 599 AGCGCC [GA]GCGC^[CT]
Hin1I 120 GACGCC G[GA]^CG[CT]C
HphI 1250 TCACC GGTGA(8/7)
MaeI 79 CTAG C^TAG
MamI 239 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 928 TGGCCA TGG^CCA
MscI 928 TGGCCA TGG^CCA
MspA1I 143 CAGCTG C[AC]G^C[GT]G
MvnI 1515 CGCG CG^CG
NaeI 123 GCCGGC GCC^GGC
NcoI 1766 CCATGG C^CATGG
NdeI 1406 CATATG CA^TATG
NgoMIV 123 GCCGGC G^CCGGC
NsiI 1409 ATGCAT ATGCA^T
PflMI 528 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1242 GACTC GAGTC(4/5)
PmaCI 1521 CACGTG CAC^GTG
PmlI 1521 CACGTG CAC^GTG
PpuMI 1296 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1582 AACGTT AA^CGTT
PvuII 143 CAGCTG CAG^CTG
SmaI 625 CCCGGG CCC^GGG
StuI 606 AGGCCT AGG^CCT
Van91I 528 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 897 CCATCCTCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 625 CCCGGG C^CCGGG
XmaI 625 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1116 GTCTAC GT^[AC][GT]AC
1742 GTATAC
AcsI 1369 GAATTT [GA]^AATT[CT]
1618 GAATTC
AflIII 769 ACATGT A^C[GA][CT]GT
1899 ACACGT
ApoI 1369 GAATTT [GA]^AATT[CT]
1618 GAATTC
AspHI 710 GTGCTC G[AT]GC[AT]^C
883 GAGCAC
AvaII 107 GGTCC G^G[AT]CC
1297 GGACC
BbsI 92 GTCTTC GAAGAC(2/6)
1553 GTCTTC
BbvCI 367 CCTCAGC CCTCAGC(-5/-2)
1138 CCTCAGC
BcnI 625 CCCGG CC^[GC]GG
626 CCGGG
BglII 1469 AGATCT A^GATCT
1870 AGATCT
BpuAI 92 GTCTTC GAAGAC(2/6)
1553 GTCTTC
BsgI 785 CTGCAC GTGCAG(16/14)
1695 CTGCAC
BsiHKAI 710 GTGCTC G[AT]GC[AT]^C
883 GAGCAC
Bsp1407I 1041 TGTACA T^GTACA
1896 TGTACA
BsrGI 1041 TGTACA T^GTACA
1896 TGTACA
BsrI 1478 ACTGG ACTGG(1/-1)
2011 CCAGT
DsaI 128 CCGTGG C^C[GA][CT]GG
1766 CCATGG
HapII 124 CCGG C^CGG
626 CCGG
HpaII 124 CCGG C^CGG
626 CCGG
MspI 124 CCGG C^CGG
626 CCGG
NciI 625 CCCGG CC^[GC]GG
626 CCGGG
PstI 682 CTGCAG CTGCA^G
719 CTGCAG
SmlI 311 CTCAAG C^T[CT][GA]AG
659 CTCAAG
SphI 990 GCATGC GCATG^C
1283 GCATGC
SspBI 1041 TGTACA T^GTACA
1896 TGTACA
TaqI 1436 TCGA T^CGA
1919 TCGA
TthHB8I 1436 TCGA T^CGA
1919 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HinfI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PshAI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII