Restriction map for
pF1KB6243
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccII AcyI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI AspI AviII BalI BbeI
BcgI BfrI BglI BglII BlpI
Bpu1102I BsaHI BsaI BsiEI BsiWI
BsmBI BspDI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 416 GGTACC G^GTACC
AccIII 387 TCCGGA T^CCGGA
AclI 681 AACGTT AA^CGTT
AhdI 2179 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 577 GTGCAC G^TGCAC
AocI 558 CCTCAGG CC^T[ACGT]AGG
ApaI 2002 GGGCCC GGGCC^C
ApaLI 577 GTGCAC G^TGCAC
Asp718I 416 GGTACC G^GTACC
AspEI 2179 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2050 CCCGGG C^[CT]CG[GA]G
AvrII 1344 CCTAGG C^CTAGG
BamHI 563 GGATCC G^GATCC
BbrPI 568 CACGTG CAC^GTG
BbsI 483 GAAGAC GAAGAC(2/6)
BciVI 990 GGATAC GTATCC(6/5)
BclI 1868 TGATCA T^GATCA
BlnI 1344 CCTAGG C^CTAGG
BpuAI 483 GAAGAC GAAGAC(2/6)
BsaAI 568 CACGTG [CT]AC^GT[GA]
BsaWI 387 TCCGGA [AT]^CCGG[AT]
BseAI 387 TCCGGA T^CCGGA
BsmI 628 GCATTC GAATGC(1/-1)
BsoBI 2050 CCCGGG C^[CT]CG[GA]G
Bsp1407I 641 TGTACA T^GTACA
BspEI 387 TCCGGA T^CCGGA
BsrBI 1930 GAGCGG CCGCTC(-3/-3)
BsrDI 262 GCAATG GCAATG(2/0)
BsrGI 641 TGTACA T^GTACA
BstBI 749 TTCGAA TT^CGAA
BstXI 1842 CCAGCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 558 CCTCAGG CC^T[ACGT]AGG
CfoI 2023 GCGC GCG^C
DraIII 725 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2179 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 558 CCTCAGG CC^T[ACGT]AGG
EcoRI 1128 GAATTC G^AATTC
FbaI 1868 TGATCA T^GATCA
HhaI 2023 GCGC GCG^C
HinP1I 2023 GCGC G^CGC
KpnI 416 GGTACC GGTAC^C
MroI 387 TCCGGA T^CCGGA
NspV 749 TTCGAA TT^CGAA
PflMI 2169 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 568 CACGTG CAC^GTG
PmlI 568 CACGTG CAC^GTG
Psp1406I 681 AACGTT AA^CGTT
PvuII 1541 CAGCTG CAG^CTG
SacI 1833 GAGCTC GAGCT^C
ScaI 1570 AGTACT AGT^ACT
SfuI 749 TTCGAA TT^CGAA
SmaI 2050 CCCGGG CCC^GGG
SmlI 1020 CTTGAG C^T[CT][GA]AG
SphI 1909 GCATGC GCATG^C
SspBI 641 TGTACA T^GTACA
SspI 349 AATATT AAT^ATT
TaqI 750 TCGA T^CGA
TthHB8I 750 TCGA T^CGA
Van91I 2169 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2050 CCCGGG C^CCGGG
XmaI 2050 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 577 GTGCAC G[AT]GC[AT]^C
1833 GAGCTC
BbvCI 1083 CCTCAGC CCTCAGC(-5/-2)
1310 CCTCAGC
BsaBI 991 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1869 GATCATCATC
BsgI 280 GTGCAG GTGCAG(16/14)
610 GTGCAG
BsiHKAI 577 GTGCAC G[AT]GC[AT]^C
1833 GAGCTC
BsmAI 2104 GAGAC GTCTC(1/5)
914 GTCTC
BspHI 255 TCATGA T^CATGA
936 TCATGA
BspMI 467 ACCTGC ACCTGC(4/8)
1305 ACCTGC
BstYI 563 GGATCC [GA]^GATC[CT]
1155 AGATCC
DsaI 1592 CCATGG C^C[GA][CT]GG
2141 CCATGG
FauI 443 CCCGC CCCGC(4/6)
1932 GCGGG
MamI 991 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1869 GATCATCATC
MflI 563 GGATCC [GA]^GATC[CT]
1155 AGATCC
MspA1I 1526 CAGCGG C[AC]G^C[GT]G
1541 CAGCTG
NcoI 1592 CCATGG C^CATGG
2141 CCATGG
NspI 1891 GCATGT [GA]CATG^[CT]
1909 GCATGC
RcaI 255 TCATGA T^CATGA
936 TCATGA
XcmI 1688 CCAGCCTAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1989 CCAGGCTCCTGATGG
XhoII 563 GGATCC [GA]^GATC[CT]
1155 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI