Restriction map for
pF1KB6249
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BbrPI BclI
BfrI BsiEI BsiWI BsmBI BspDI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HgaI HindIII HpaI KpnI KspI
MfeI MluI MunI MvnI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 607 GTCGAC GT^[AC][GT]AC
AccIII 311 TCCGGA T^CCGGA
AcsI 1407 GAATTC [GA]^AATT[CT]
AcyI 258 GGCGCC G[GA]^CG[CT]C
AflIII 31 ACATGT A^C[GA][CT]GT
AocI 238 CCTGAGG CC^T[ACGT]AGG
ApaI 883 GGGCCC GGGCC^C
ApoI 1407 GAATTC [GA]^AATT[CT]
AvrII 498 CCTAGG C^CTAGG
BalI 281 TGGCCA TGG^CCA
BamHI 870 GGATCC G^GATCC
BbeI 258 GGCGCC GGCGC^C
BbvCI 1275 GCTGAGG CCTCAGC(-5/-2)
BciVI 1235 GTATCC GTATCC(6/5)
BglI 1649 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 498 CCTAGG C^CTAGG
BsaAI 876 CACGTA [CT]AC^GT[GA]
BsaHI 258 GGCGCC G[GA]^CG[CT]C
BsaI 1736 GAGACC GGTCTC(1/5)
BsaWI 311 TCCGGA [AT]^CCGG[AT]
BseAI 311 TCCGGA T^CCGGA
Bsp1407I 1709 TGTACA T^GTACA
BspEI 311 TCCGGA T^CCGGA
BspLU11I 31 ACATGT A^CATGT
BspMI 269 ACCTGC ACCTGC(4/8)
BsrBI 166 GAGCGG CCGCTC(-3/-3)
BsrDI 384 CATTGC GCAATG(2/0)
BsrGI 1709 TGTACA T^GTACA
BstEII 8 GGTCACC G^GT[ACGT]ACC
BstPI 8 GGTCACC G^GT[ACGT]ACC
Bsu36I 238 CCTGAGG CC^T[ACGT]AGG
DraIII 1101 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 281 TGGCCA [CT]^GGCC[GA]
Eco81I 238 CCTGAGG CC^T[ACGT]AGG
EcoNI 2253 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 8 GGTCACC G^GT[ACGT]ACC
EcoRI 1407 GAATTC G^AATTC
Hin1I 258 GGCGCC G[GA]^CG[CT]C
HincII 607 GTCGAC GT[CT]^[GA]AC
HindII 607 GTCGAC GT[CT]^[GA]AC
KasI 258 GGCGCC G^GCGCC
MaeII 877 ACGT A^CGT
MluNI 281 TGGCCA TGG^CCA
MroI 311 TCCGGA T^CCGGA
MscI 281 TGGCCA TGG^CCA
MslI 208 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 137 GCCGGC GCC^GGC
NarI 258 GGCGCC GG^CGCC
NgoMIV 137 GCCGGC G^CCGGC
NspI 31 ACATGT [GA]CATG^[CT]
PaeR7I 1733 CTCGAG C^TCGAG
PflMI 1137 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 550 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1923 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 607 GTCGAC G^TCGAC
SapI 2239 GAAGAGC GCTCTTC(1/4)
ScaI 290 AGTACT AGT^ACT
SfoI 258 GGCGCC GGC^GCC
SspBI 1709 TGTACA T^GTACA
StuI 2261 AGGCCT AGG^CCT
Van91I 1137 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1733 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 227 CCCGAG C^[CT]CG[GA]G
1733 CTCGAG
BbsI 739 GAAGAC GAAGAC(2/6)
1920 GAAGAC
BcgI 1089 CGATAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
536 GCACATCATTCG
BcnI 155 CCCGG CC^[GC]GG
2016 CCCGG
BglII 306 AGATCT A^GATCT
1199 AGATCT
BlpI 147 GCTCAGC GC^T[ACGT]AGC
808 GCTGAGC
Bpu1102I 147 GCTCAGC GC^T[ACGT]AGC
808 GCTGAGC
BpuAI 739 GAAGAC GAAGAC(2/6)
1920 GAAGAC
BsaBI 120 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
183 GATCCAGATC
BsgI 2145 CTGCAC GTGCAG(16/14)
685 GTGCAG
BsmI 2028 GAATGC GAATGC(1/-1)
693 GCATTC
BsoBI 227 CCCGAG C^[CT]CG[GA]G
1733 CTCGAG
BspHI 191 TCATGA T^CATGA
408 TCATGA
BsrFI 137 GCCGGC [GA]^CCGG[CT]
1334 ACCGGC
BstXI 212 CCAATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
509 CCAAGGAGCTGG
CelII 147 GCTCAGC GC^T[ACGT]AGC
808 GCTGAGC
Cfr10I 137 GCCGGC [GA]^CCGG[CT]
1334 ACCGGC
MamI 120 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
183 GATCCAGATC
NciI 155 CCCGG CC^[GC]GG
2016 CCCGG
PleI 1194 GACTC GAGTC(4/5)
993 GAGTC
RcaI 191 TCATGA T^CATGA
408 TCATGA
SmlI 1435 CTCAAG C^T[CT][GA]AG
1733 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII