Restriction map for
pF1KB6956
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# NO CUT ENZYMES ( 161 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeIII HgaI HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI TspRI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 290 GACGTC GACGT^C
AccII 5 CGCG CG^CG
AciI 15 GCGG CCGC(-3/-1)
AcsI 157 GAATTC [GA]^AATT[CT]
AcyI 290 GACGTC G[GA]^CG[CT]C
AluI 519 AGCT AG^CT
ApoI 157 GAATTC [GA]^AATT[CT]
Asp700I 659 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 224 CCCGAG C^[CT]CG[GA]G
AvaII 103 GGTCC G^G[AT]CC
BcgI 245 CGACAGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 137 CTCCAG CTGGAG(16/14)
BsaHI 290 GACGTC G[GA]^CG[CT]C
BsoBI 224 CCCGAG C^[CT]CG[GA]G
BsrFI 368 GCCGGC [GA]^CCGG[CT]
BstAPI 178 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 160 TTCGAA TT^CGAA
BstEII 46 GGTAACC G^GT[ACGT]ACC
BstF5I 201 GGATG GGATG(2/0)
BstPI 46 GGTAACC G^GT[ACGT]ACC
BstUI 5 CGCG CG^CG
CfoI 62 GCGC GCG^C
Cfr10I 368 GCCGGC [GA]^CCGG[CT]
Cfr13I 103 GGTCC G^G[ACGT]CC
DdeI 526 CTCAG C^T[ACGT]AG
EarI 500 CTCTTC CTCTTC(1/4)
EcoO65I 46 GGTAACC G^GT[ACGT]ACC
EcoRI 157 GAATTC G^AATTC
EcoT14I 32 CCAAGG C^C[AT][AT]GG
FokI 201 GGATG GGATG(9/13)
HaeII 61 GGCGCT [GA]GCGC^[CT]
HapII 369 CCGG C^CGG
HhaI 62 GCGC GCG^C
Hin1I 290 GACGTC G[GA]^CG[CT]C
HinP1I 62 GCGC G^CGC
HpaII 369 CCGG C^CGG
HphI 210 GGTGA GGTGA(8/7)
Ksp632I 500 CTCTTC CTCTTC(1/4)
MseI 109 TTAA T^TAA
MspI 369 CCGG C^CGG
MvnI 5 CGCG CG^CG
NaeI 368 GCCGGC GCC^GGC
NgoMIV 368 GCCGGC G^CCGGC
NlaIII 252 CATG CATG^
NlaIV 220 GGCTCC GG[ACGT]^[ACGT]CC
NspI 251 GCATGC [GA]CATG^[CT]
NspV 160 TTCGAA TT^CGAA
PshAI 455 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau96I 103 GGTCC G^G[ACGT]CC
SfuI 160 TTCGAA TT^CGAA
SphI 251 GCATGC GCATG^C
StyI 32 CCAAGG C^C[AT][AT]GG
Tru9I 109 TTAA T^TAA
Tsp45I 387 GTCAC ^GT[GC]AC
Tsp509I 158 AATT ^AATT
XbaI 394 TCTAGA T^CTAGA
XmnI 659 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AfaI 18 GTAC GT^AC
190 GTAC
BbvI 440 GCAGC GCAGC(8/12)
623 GCAGC
BseRI 476 CTCCTC GAGGAG(10/8)
23 GAGGAG
BspMI 282 ACCTGC ACCTGC(4/8)
383 GCAGGT
BsrI 58 ACTGG ACTGG(1/-1)
198 ACTGG
Fnu4HI 440 GCAGC GC^[ACGT]GC
623 GCAGC
ItaI 440 GCAGC GC^[ACGT]GC
623 GCAGC
MaeII 267 ACGT A^CGT
291 ACGT
RsaI 18 GTAC GT^AC
190 GTAC
TseI 440 GCAGC G^C[AT]GC
623 GCAGC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AlwI BfaI BglII BsaBI BsaJI
BsiYI BslI BsmFI BssKI BstNI
BstYI Cac8I DpnII EcoRII MaeI
MaeIII MamI MboI MboII MflI
MnlI MvaI MwoI NdeII Sau3AI
ScrFI SfaNI TaqI TthHB8I XhoII