Restriction map for
pF1KB6284
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI Asp700I AspEI AvrII
BbeI BbrPI BbvCI BciVI BfrI
BglII BlnI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KasI
KspI MaeII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 2160 AAATTC [GA]^AATT[CT]
AcyI 1882 GACGCC G[GA]^CG[CT]C
Alw44I 2020 GTGCAC G^TGCAC
ApaLI 2020 GTGCAC G^TGCAC
ApoI 2160 AAATTC [GA]^AATT[CT]
AseI 243 ATTAAT AT^TAAT
AsnI 243 ATTAAT AT^TAAT
AspHI 2020 GTGCAC G[AT]GC[AT]^C
AspI 1112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 653 TGCGCA TGC^GCA
BbsI 1010 GAAGAC GAAGAC(2/6)
BcgI 1810 GCAGCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 459 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1010 GAAGAC GAAGAC(2/6)
BsaHI 1882 GACGCC G[GA]^CG[CT]C
BsiHKAI 2020 GTGCAC G[AT]GC[AT]^C
Bsp1407I 527 TGTACA T^GTACA
BsrDI 272 GCAATG GCAATG(2/0)
BsrFI 1658 ACCGGC [GA]^CCGG[CT]
BsrGI 527 TGTACA T^GTACA
Cfr10I 1658 ACCGGC [GA]^CCGG[CT]
DrdI 1429 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1470 CCATGG C^C[GA][CT]GG
EarI 1419 CTCTTC CTCTTC(1/4)
FspI 653 TGCGCA TGC^GCA
HaeII 1208 AGCGCC [GA]GCGC^[CT]
Hin1I 1882 GACGCC G[GA]^CG[CT]C
Ksp632I 1419 CTCTTC CTCTTC(1/4)
MseI 244 TTAA T^TAA
NcoI 1470 CCATGG C^CATGG
NheI 367 GCTAGC G^CTAGC
PaeR7I 2323 CTCGAG C^TCGAG
PflFI 1112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 243 ATTAAT AT^TAAT
SapI 1418 GCTCTTC GCTCTTC(1/4)
SbfI 60 CCTGCAGG CCTGCA^GG
SmlI 2323 CTCGAG C^T[CT][GA]AG
Sse8387I 60 CCTGCAGG CCTGCA^GG
SspBI 527 TGTACA T^GTACA
Tru9I 244 TTAA T^TAA
Tth111I 1112 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2323 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 740 GGTACC G^GTACC
1367 GGTACC
Asp718I 740 GGTACC G^GTACC
1367 GGTACC
BclI 632 TGATCA T^GATCA
1782 TGATCA
BlpI 442 GCTGAGC GC^T[ACGT]AGC
800 GCTCAGC
Bpu1102I 442 GCTGAGC GC^T[ACGT]AGC
800 GCTCAGC
BsaWI 659 TCCGGT [AT]^CCGG[AT]
1717 ACCGGA
BstAPI 656 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2261 GCAGGCCATGC
BstXI 818 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1686 CCAGTGGCATGG
CelII 442 GCTGAGC GC^T[ACGT]AGC
800 GCTCAGC
CfoI 654 GCGC GCG^C
1209 GCGC
FbaI 632 TGATCA T^GATCA
1782 TGATCA
HhaI 654 GCGC GCG^C
1209 GCGC
HinP1I 654 GCGC G^CGC
1209 GCGC
HincII 1438 GTCAAC GT[CT]^[GA]AC
1879 GTTGAC
HindII 1438 GTCAAC GT[CT]^[GA]AC
1879 GTTGAC
KpnI 740 GGTACC GGTAC^C
1367 GGTACC
MboII 1010 GAAGA GAAGA(8/7)
1420 TCTTC
MslI 624 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1228 CACCCCTATG
NspI 935 GCATGT [GA]CATG^[CT]
1562 ACATGC
PpuMI 978 GGGACCC [GA]G^G[AT]CC[CT]
1974 GGGTCCC
SexAI 1002 ACCAGGT A^CC[AT]GGT
1113 ACCTGGT
SmaI 764 CCCGGG CCC^GGG
1187 CCCGGG
StuI 1735 AGGCCT AGG^CCT
2112 AGGCCT
Tsp509I 242 AATT ^AATT
2161 AATT
XcmI 716 CCATCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1424 CCAGTGACTCTGTGG
XmaCI 764 CCCGGG C^CCGGG
1187 CCCGGG
XmaI 764 CCCGGG C^CCGGG
1187 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BalI BamHI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XhoII