Restriction map for
pF1KB7920
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BbeI BbrPI
BbsI BcgI BclI BfrI BglI
BglII BlnI BpuAI BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmI
BspDI BspHI BspLU11I BsrBI BssHII
BssSI BstBI BstEII BstPI BstXI
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FauI FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MseI
MunI NarI NcoI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tsp45I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 504 GTATAC GT^[AC][GT]AC
AccII 37 CGCG CG^CG
AccIII 863 TCCGGA T^CCGGA
Asp700I 138 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 899 CCCGGG C^[CT]CG[GA]G
BamHI 524 GGATCC G^GATCC
BanI 249 GGTGCC G^G[CT][GA]CC
BciVI 840 GGATAC GTATCC(6/5)
BlpI 598 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 598 GCTCAGC GC^T[ACGT]AGC
BsaAI 487 TACGTG [CT]AC^GT[GA]
BseAI 863 TCCGGA T^CCGGA
BseRI 751 GAGGAG GAGGAG(10/8)
BsgI 790 GTGCAG GTGCAG(16/14)
BsmAI 422 GTCTC GTCTC(1/5)
BsmBI 421 CGTCTC CGTCTC(1/5)
BsmFI 119 GGGAC GGGAC(10/14)
BsoBI 899 CCCGGG C^[CT]CG[GA]G
Bsp1407I 281 TGTACA T^GTACA
BspEI 863 TCCGGA T^CCGGA
BsrFI 998 GCCGGC [GA]^CCGG[CT]
BsrGI 281 TGTACA T^GTACA
Bst1107I 504 GTATAC GTA^TAC
BstAPI 208 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 37 CGCG CG^CG
BstYI 524 GGATCC [GA]^GATC[CT]
BstZ17I 504 GTATAC GTA^TAC
CelII 598 GCTCAGC GC^T[ACGT]AGC
CfoI 1002 GCGC GCG^C
Cfr10I 998 GCCGGC [GA]^CCGG[CT]
DraII 272 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 731 CCGTGG C^C[GA][CT]GG
EcoO109I 272 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 233 GAATTC G^AATTC
EcoRV 586 GATATC GAT^ATC
HaeII 1001 GGCGCT [GA]GCGC^[CT]
HgaI 205 GACGC GACGC(5/10)
HhaI 1002 GCGC GCG^C
HinP1I 1002 GCGC G^CGC
HincII 933 GTCAAC GT[CT]^[GA]AC
HindII 933 GTCAAC GT[CT]^[GA]AC
MaeII 488 ACGT A^CGT
MflI 524 GGATCC [GA]^GATC[CT]
MroI 863 TCCGGA T^CCGGA
MslI 163 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 37 CGCG CG^CG
NaeI 998 GCCGGC GCC^GGC
NdeI 514 CATATG CA^TATG
NgoMIV 998 GCCGGC G^CCGGC
NheI 240 GCTAGC G^CTAGC
PleI 931 GAGTC GAGTC(4/5)
PpuMI 272 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 981 AGTACT AGT^ACT
SexAI 774 ACCTGGT A^CC[AT]GGT
SfcI 412 CTACAG C^T[GA][CT]AG
SmaI 899 CCCGGG CCC^GGG
SmlI 724 CTTGAG C^T[CT][GA]AG
SspBI 281 TGTACA T^GTACA
XcmI 827 CCAACCCCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 524 GGATCC [GA]^GATC[CT]
XmaCI 899 CCCGGG C^CCGGG
XmaI 899 CCCGGG C^CCGGG
XmnI 138 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 141 GAATTT [GA]^AATT[CT]
233 GAATTC
ApoI 141 GAATTT [GA]^AATT[CT]
233 GAATTC
BalI 612 TGGCCA TGG^CCA
815 TGGCCA
BanII 331 GGGCTC G[GA]GC[CT]^C
727 GAGCCC
BbvCI 244 GCTGAGG CCTCAGC(-5/-2)
748 GCTGAGG
BfaI 241 CTAG C^TAG
1018 CTAG
BpmI 424 CTCCAG CTGGAG(16/14)
181 CTGGAG
BsaWI 100 TCCGGT [AT]^CCGG[AT]
863 TCCGGA
BspMI 626 ACCTGC ACCTGC(4/8)
761 GCAGGT
BsrDI 213 CATTGC GCAATG(2/0)
65 GCAATG
DraIII 496 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
773 CACCTGGTG
EaeI 612 TGGCCA [CT]^GGCC[GA]
815 TGGCCA
EcoT14I 378 CCAAGG C^C[AT][AT]GG
801 CCAAGG
MaeI 241 CTAG C^TAG
1018 CTAG
MaeIII 92 GTAAC ^GT[ACGT]AC
695 GTTAC
MluNI 612 TGGCCA TGG^CCA
815 TGGCCA
MscI 612 TGGCCA TGG^CCA
815 TGGCCA
MspA1I 601 CAGCTG C[AC]G^C[GT]G
746 CAGCTG
PvuII 601 CAGCTG CAG^CTG
746 CAGCTG
StyI 378 CCAAGG C^C[AT][AT]GG
801 CCAAGG
TfiI 312 GATTC G^A[AT]TC
463 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp509I TspRI TthHB8I