Restriction map for
pF1KB7927
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BseAI BsiEI
BsiWI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRII
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MunI MvaI
NaeI NarI NciI NcoI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 12 CGCG CG^CG
AcsI 102 GAATTT [GA]^AATT[CT]
AfaI 399 GTAC GT^AC
AflIII 447 ACATGT A^C[GA][CT]GT
AlwI 370 GATCC GGATC(4/5)
AocI 211 CCTAAGG CC^T[ACGT]AGG
ApoI 102 GAATTT [GA]^AATT[CT]
Asp700I 583 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 461 GAGCAC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BbsI 549 GTCTTC GAAGAC(2/6)
BglII 87 AGATCT A^GATCT
BpuAI 549 GTCTTC GAAGAC(2/6)
BsaWI 218 TCCGGT [AT]^CCGG[AT]
BsgI 16 GTGCAG GTGCAG(16/14)
BsiHKAI 461 GAGCAC G[AT]GC[AT]^C
BsmBI 61 GAGACG CGTCTC(1/5)
BsmI 528 GAATGC GAATGC(1/-1)
BspLU11I 447 ACATGT A^CATGT
BsrI 466 CCAGT ACTGG(1/-1)
BstUI 12 CGCG CG^CG
BstYI 87 AGATCT [GA]^GATC[CT]
Bsu36I 211 CCTAAGG CC^T[ACGT]AGG
Cac8I 339 GCAAGC GC[ACGT]^[ACGT]GC
DraII 132 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 51 GAAGAG CTCTTC(1/4)
Eco81I 211 CCTAAGG CC^T[ACGT]AGG
EcoO109I 132 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 280 GATATC GAT^ATC
FauI 30 GCGGG CCCGC(4/6)
HapII 219 CCGG C^CGG
HpaII 219 CCGG C^CGG
Ksp632I 51 GAAGAG CTCTTC(1/4)
MflI 87 AGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspI 219 CCGG C^CGG
MvnI 12 CGCG CG^CG
NdeI 149 CATATG CA^TATG
NspI 447 ACATGT [GA]CATG^[CT]
PleI 312 GACTC GAGTC(4/5)
PpuMI 132 AGGACCT [GA]G^G[AT]CC[CT]
PstI 356 CTGCAG CTGCA^G
RsaI 399 GTAC GT^AC
SapI 51 GAAGAGC GCTCTTC(1/4)
SspI 247 AATATT AAT^ATT
TaqI 435 TCGA T^CGA
TfiI 23 GAATC G^A[AT]TC
Tsp45I 259 GTCAC ^GT[GC]AC
TthHB8I 435 TCGA T^CGA
XhoII 87 AGATCT [GA]^GATC[CT]
XmnI 583 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvaII 133 GGACC G^G[AT]CC
216 GGTCC
BfaI 172 CTAG C^TAG
473 CTAG
BmyI 443 GGGCAC G[AGT]GC[ACT]^C
461 GAGCAC
BpmI 137 CTCCAG CTGGAG(16/14)
458 CTGGAG
BseRI 58 GAGGAG GAGGAG(10/8)
119 GAGGAG
BsiYI 148 CCATATGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
257 CCGTCACAGGG
BslI 148 CCATATGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
257 CCGTCACAGGG
BsmAI 61 GAGAC GTCTC(1/5)
571 GAGAC
Bsp1286I 443 GGGCAC G[AGT]GC[ACT]^C
461 GAGCAC
BsrDI 335 CATTGC GCAATG(2/0)
307 GCAATG
Cfr13I 133 GGACC G^G[ACGT]CC
216 GGTCC
DdeI 212 CTAAG C^T[ACGT]AG
592 CTGAG
HaeIII 3 GGCC GG^CC
543 GGCC
HinfI 23 GAATC G^A[ACGT]TC
312 GACTC
MaeI 172 CTAG C^TAG
473 CTAG
MaeIII 259 GTCAC ^GT[ACGT]AC
596 GTAAC
MslI 9 CATCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
448 CATGTGTATG
MspA1I 302 CAGCTG C[AC]G^C[GT]G
353 CAGCTG
NlaIII 448 CATG CATG^
560 CATG
PvuII 302 CAGCTG CAG^CTG
353 CAGCTG
Sau96I 133 GGACC G^G[ACGT]CC
216 GGTCC
SfaNI 379 GATGC GCATC(5/9)
240 GCATC
SfcI 356 CTGCAG C^T[GA][CT]AG
383 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AluI BbvI BstF5I DpnII
Fnu4HI FokI ItaI MboI MboII
MnlI MseI MwoI NdeII Sau3AI
Tru9I TseI Tsp509I