Restriction map for
pF1KB7929
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BanII BbrPI BbvCI BcgI
BciVI BclI BglI BglII BlnI
BmyI BpmI BsaBI BsaI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 374 GACGTC GACGT^C
AccII 245 CGCG CG^CG
AfaI 288 GTAC GT^AC
AflII 382 CTTAAG C^TTAAG
BalI 704 TGGCCA TGG^CCA
BamHI 853 GGATCC G^GATCC
BbsI 13 GTCTTC GAAGAC(2/6)
BcnI 191 CCCGG CC^[GC]GG
BfaI 340 CTAG C^TAG
BfrI 382 CTTAAG C^TTAAG
BpuAI 13 GTCTTC GAAGAC(2/6)
BsaWI 176 ACCGGA [AT]^CCGG[AT]
BsmFI 1005 GTCCC GGGAC(10/14)
BsmI 131 GCATTC GAATGC(1/-1)
BspLU11I 696 ACATGT A^CATGT
BspMI 345 GCAGGT ACCTGC(4/8)
BsrDI 455 GCAATG GCAATG(2/0)
BstAPI 225 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 99 CCTGG CC^[AT]GG
BstUI 245 CGCG CG^CG
DraII 227 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 227 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 582 GAATTC G^AATTC
EcoRII 99 CCTGG ^CC[AT]GG
FseI 79 GGCCGGCC GGCCGG^CC
HgaI 336 GACGC GACGC(5/10)
MaeI 340 CTAG C^TAG
MluNI 704 TGGCCA TGG^CCA
MscI 704 TGGCCA TGG^CCA
MslI 714 CACTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 99 CCTGG CC^[AT]GG
MvnI 245 CGCG CG^CG
NaeI 80 GCCGGC GCC^GGC
NciI 191 CCCGG CC^[GC]GG
NgoMIV 80 GCCGGC G^CCGGC
NspI 696 ACATGT [GA]CATG^[CT]
PstI 970 CTGCAG CTGCA^G
PvuII 984 CAGCTG CAG^CTG
RsaI 288 GTAC GT^AC
SfaNI 989 GCATC GCATC(5/9)
TaqI 396 TCGA T^CGA
TthHB8I 396 TCGA T^CGA
XcmI 403 CCAGATTTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 582 GAATTC [GA]^AATT[CT]
772 GAATTT
AflIII 696 ACATGT A^C[GA][CT]GT
905 ACACGT
AlwNI 226 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
806 CAGAAACTG
ApoI 582 GAATTC [GA]^AATT[CT]
772 GAATTT
AvaI 121 CCCGAG C^[CT]CG[GA]G
750 CTCGGG
AvaII 182 GGACC G^G[AT]CC
889 GGACC
BbeI 55 GGCGCC GGCGC^C
187 GGCGCC
BlpI 24 GCTCAGC GC^T[ACGT]AGC
468 GCTTAGC
Bpu1102I 24 GCTCAGC GC^T[ACGT]AGC
468 GCTTAGC
BsaAI 634 TACGTG [CT]AC^GT[GA]
906 CACGTA
BsgI 343 GTGCAG GTGCAG(16/14)
1045 GTGCAG
BsoBI 121 CCCGAG C^[CT]CG[GA]G
750 CTCGGG
BsrBI 64 GAGCGG CCGCTC(-3/-3)
70 GAGCGG
BsrFI 80 GCCGGC [GA]^CCGG[CT]
184 ACCGGC
BsrI 553 CCAGT ACTGG(1/-1)
623 CCAGT
BssKI 99 CCTGG ^CC[ACGT]GG
191 CCCGG
BstYI 754 GGATCT [GA]^GATC[CT]
853 GGATCC
CelII 24 GCTCAGC GC^T[ACGT]AGC
468 GCTTAGC
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
184 ACCGGC
EaeI 78 TGGCCG [CT]^GGCC[GA]
704 TGGCCA
HaeII 55 GGCGCC [GA]GCGC^[CT]
187 GGCGCC
KasI 55 GGCGCC G^GCGCC
187 GGCGCC
MflI 754 GGATCT [GA]^GATC[CT]
853 GGATCC
NarI 55 GGCGCC GG^CGCC
187 GGCGCC
ScrFI 99 CCTGG CC^[ACGT]GG
191 CCCGG
SfcI 87 CTACAG C^T[GA][CT]AG
970 CTGCAG
SfoI 55 GGCGCC GGC^GCC
187 GGCGCC
Tsp45I 278 GTGAC ^GT[GC]AC
615 GTCAC
XhoII 754 GGATCT [GA]^GATC[CT]
853 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcyI AluI AlwI BanI
BbvI BsaHI BsaJI BsiYI BslI
BsmAI BstF5I Cac8I CfoI Cfr13I
DdeI DpnII FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I SmlI
Tru9I TseI Tsp509I TspRI