Restriction map for
pF1KB7939
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AfaI 408 GTAC GT^AC
AocI 856 CCTGAGG CC^T[ACGT]AGG
Asp700I 142 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 272 CCCGGG C^[CT]CG[GA]G
AvaII 983 GGTCC G^G[AT]CC
BalI 919 TGGCCA TGG^CCA
BanI 686 GGTGCC G^G[CT][GA]CC
BanII 528 GAGCCC G[GA]GC[CT]^C
BmyI 528 GAGCCC G[AGT]GC[ACT]^C
BpmI 978 CTGGAG CTGGAG(16/14)
BsmAI 875 GAGAC GTCTC(1/5)
BsmI 25 GAATGC GAATGC(1/-1)
BsoBI 272 CCCGGG C^[CT]CG[GA]G
Bsp1286I 528 GAGCCC G[AGT]GC[ACT]^C
BspMI 786 ACCTGC ACCTGC(4/8)
BsrI 216 CCAGT ACTGG(1/-1)
BssSI 956 CTCGTG CACGAG(-5/-1)
BstEII 63 GGTAACC G^GT[ACGT]ACC
BstPI 63 GGTAACC G^GT[ACGT]ACC
Bsu36I 856 CCTGAGG CC^T[ACGT]AGG
Cac8I 167 GCGTGC GC[ACGT]^[ACGT]GC
DraII 87 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 919 TGGCCA [CT]^GGCC[GA]
EarI 889 GAAGAG CTCTTC(1/4)
Eco81I 856 CCTGAGG CC^T[ACGT]AGG
EcoO109I 87 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 63 GGTAACC G^GT[ACGT]ACC
EcoRI 662 GAATTC G^AATTC
HphI 784 TCACC GGTGA(8/7)
Ksp632I 889 GAAGAG CTCTTC(1/4)
MluNI 919 TGGCCA TGG^CCA
MscI 919 TGGCCA TGG^CCA
MslI 454 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1008 CAGCTG C[AC]G^C[GT]G
NcoI 234 CCATGG C^CATGG
PleI 949 GAGTC GAGTC(4/5)
PvuII 1008 CAGCTG CAG^CTG
RsaI 408 GTAC GT^AC
SfaNI 479 GCATC GCATC(5/9)
SmaI 272 CCCGGG CCC^GGG
SmlI 641 CTCAAG C^T[CT][GA]AG
Tsp45I 422 GTGAC ^GT[GC]AC
XmaCI 272 CCCGGG C^CCGGG
XmaI 272 CCCGGG C^CCGGG
XmnI 142 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 558 CCGC CCGC(-3/-1)
710 GCGG
AcsI 142 GAATTT [GA]^AATT[CT]
662 GAATTC
AlwNI 86 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
729 CAGGGACTG
ApoI 142 GAATTT [GA]^AATT[CT]
662 GAATTC
BamHI 123 GGATCC G^GATCC
712 GGATCC
BglII 287 AGATCT A^GATCT
748 AGATCT
BsmFI 731 GGGAC GGGAC(10/14)
824 GGGAC
BstNI 118 CCTGG CC^[AT]GG
977 CCTGG
DsaI 234 CCATGG C^C[GA][CT]GG
717 CCACGG
EcoRII 118 CCTGG ^CC[AT]GG
977 CCTGG
HapII 273 CCGG C^CGG
898 CCGG
HpaII 273 CCGG C^CGG
898 CCGG
MspI 273 CCGG C^CGG
898 CCGG
MvaI 118 CCTGG CC^[AT]GG
977 CCTGG
NspI 459 GCATGT [GA]CATG^[CT]
706 ACATGC
TaqI 111 TCGA T^CGA
627 TCGA
TfiI 894 GAATC G^A[AT]TC
998 GAATC
TspRI 340 CAGTG CA[GC]TG[ACGT][ACGT]^
616 CAGTG
TthHB8I 111 TCGA T^CGA
627 TCGA
XcmI 357 CCATCTGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
673 CCAGACAGGAGGTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AluI AlwI BbsI BbvI BcnI
BfaI BpuAI BsaJI BseRI BsiYI
BslI BssKI BstF5I BstYI Cfr13I
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MwoI
NciI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfcI StyI
TseI Tsp509I XhoII