################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 184 AAATTC [GA]^AATT[CT] AfaI 105 GTAC GT^AC Alw44I 291 GTGCAC G^TGCAC AlwNI 625 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 291 GTGCAC G^TGCAC ApoI 184 AAATTC [GA]^AATT[CT] AscI 436 GGCGCGCC GG^CGCGCC AspHI 291 GTGCAC G[AT]GC[AT]^C BalI 1022 TGGCCA TGG^CCA BamHI 678 GGATCC G^GATCC BanII 570 GAGCCC G[GA]GC[CT]^C BbvCI 999 GCTGAGG CCTCAGC(-5/-2) BsaWI 295 ACCGGA [AT]^CCGG[AT] BseRI 835 GAGGAG GAGGAG(10/8) BsgI 480 CTGCAC GTGCAG(16/14) BsiEI 420 CGACCG CG[GA][CT]^CG BsiHKAI 291 GTGCAC G[AT]GC[AT]^C BsmBI 299 GAGACG CGTCTC(1/5) BspMI 977 ACCTGC ACCTGC(4/8) BssHII 437 GCGCGC G^CGCGC BstAPI 1143 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 648 GGTCACC G^GT[ACGT]ACC BstPI 648 GGTCACC G^GT[ACGT]ACC BstYI 678 GGATCC [GA]^GATC[CT] CpoI 48 CGGTCCG CG^G[AT]CCG EaeI 1022 TGGCCA [CT]^GGCC[GA] EcoNI 1056 CCTAAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 648 GGTCACC G^GT[ACGT]ACC HgaI 463 GCGTC GACGC(5/10) HphI 650 TCACC GGTGA(8/7) MaeII 211 ACGT A^CGT MflI 678 GGATCC [GA]^GATC[CT] MluNI 1022 TGGCCA TGG^CCA MscI 1022 TGGCCA TGG^CCA NruI 985 TCGCGA TCG^CGA NspI 371 GCATGT [GA]CATG^[CT] PstI 815 CTGCAG CTGCA^G PvuII 1026 CAGCTG CAG^CTG RsaI 105 GTAC GT^AC RsrII 48 CGGTCCG CG^G[AT]CCG SexAI 1007 ACCAGGT A^CC[AT]GGT TspRI 1173 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 678 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 438 CGCG CG^CG 986 CGCG BbeI 159 GGCGCC GGCGC^C 510 GGCGCC BsmAI 299 GAGAC GTCTC(1/5) 627 GTCTC BsrI 1174 ACTGG ACTGG(1/-1) 885 CCAGT BstUI 438 CGCG CG^CG 986 CGCG DpnII 679 GATC ^GATC 1053 GATC DsaI 155 CCACGG C^C[GA][CT]GG 277 CCGTGG KasI 159 GGCGCC G^GCGCC 510 GGCGCC MboI 679 GATC ^GATC 1053 GATC MslI 654 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 724 CACAACTATG MspA1I 529 CCGCTG C[AC]G^C[GT]G 1026 CAGCTG MvnI 438 CGCG CG^CG 986 CGCG NarI 159 GGCGCC GG^CGCC 510 GGCGCC NdeII 679 GATC ^GATC 1053 GATC Sau3AI 679 GATC ^GATC 1053 GATC SfaNI 811 GATGC GCATC(5/9) 1133 GATGC SfoI 159 GGCGCC GGC^GCC 510 GGCGCC StuI 551 AGGCCT AGG^CCT 1064 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcyI AluI AlwI Asp700I AvaI AvaII BanI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboII MnlI MspI MvaI MwoI NaeI NciI NgoMIV NlaIII NlaIV PleI PpuMI Sau96I ScrFI SfcI SmaI StyI TaqI TfiI TseI Tsp45I Tsp509I TthHB8I XmaCI XmaI XmnI