Restriction map for
pF1KB9909
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpmI BpuAI BsaBI
BsaI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MseI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 30 GGCGTC G[GA]^CG[CT]C
BalI 458 TGGCCA TGG^CCA
BamHI 725 GGATCC G^GATCC
BcgI 590 GCATCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 715 TACGTG [CT]AC^GT[GA]
BsaHI 30 GGCGTC G[GA]^CG[CT]C
BseRI 358 CTCCTC GAGGAG(10/8)
BsiEI 203 CGGCCG CG[GA][CT]^CG
BsmAI 602 GAGAC GTCTC(1/5)
BsrBI 57 GAGCGG CCGCTC(-3/-3)
BssSI 94 CACGAG CACGAG(-5/-1)
BstEII 531 GGTCACC G^GT[ACGT]ACC
BstPI 531 GGTCACC G^GT[ACGT]ACC
BstYI 725 GGATCC [GA]^GATC[CT]
DraII 503 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 203 CGGCCG C^GGCCG
EclXI 203 CGGCCG C^GGCCG
Eco52I 203 CGGCCG C^GGCCG
EcoO109I 503 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 531 GGTCACC G^GT[ACGT]ACC
HaeII 233 AGCGCC [GA]GCGC^[CT]
Hin1I 30 GGCGTC G[GA]^CG[CT]C
MflI 725 GGATCC [GA]^GATC[CT]
MluNI 458 TGGCCA TGG^CCA
MscI 458 TGGCCA TGG^CCA
NcoI 461 CCATGG C^CATGG
PpuMI 503 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 514 GAGCTC GAGCT^C
SbfI 507 CCTGCAGG CCTGCA^GG
SmaI 150 CCCGGG CCC^GGG
Sse8387I 507 CCTGCAGG CCTGCA^GG
XcmI 17 CCAGCCCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 725 GGATCC [GA]^GATC[CT]
XmaCI 150 CCCGGG C^CCGGG
XmaI 150 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 250 GTGCTC G[AT]GC[AT]^C
514 GAGCTC
AvaI 68 CCCGAG C^[CT]CG[GA]G
150 CCCGGG
AvaII 426 GGACC G^G[AT]CC
504 GGTCC
BlpI 54 GCTGAGC GC^T[ACGT]AGC
369 GCTGAGC
Bpu1102I 54 GCTGAGC GC^T[ACGT]AGC
369 GCTGAGC
BsiHKAI 250 GTGCTC G[AT]GC[AT]^C
514 GAGCTC
BsmFI 221 GGGAC GGGAC(10/14)
571 GTCCC
BsoBI 68 CCCGAG C^[CT]CG[GA]G
150 CCCGGG
BstAPI 210 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
446 GCACCCAGTGC
CelII 54 GCTGAGC GC^T[ACGT]AGC
369 GCTGAGC
DraIII 262 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
447 CACCCAGTG
HinfI 604 GACTC G^A[ACGT]TC
649 GACTC
MaeIII 523 GTCAC ^GT[ACGT]AC
532 GTCAC
MboII 539 TCTTC GAAGA(8/7)
695 TCTTC
MspA1I 367 CAGCTG C[AC]G^C[GT]G
412 CAGCTG
NaeI 545 GCCGGC GCC^GGC
586 GCCGGC
NgoMIV 545 GCCGGC G^CCGGC
586 GCCGGC
NlaIII 123 CATG CATG^
462 CATG
PleI 604 GACTC GAGTC(4/5)
649 GACTC
PstI 133 CTGCAG CTGCA^G
508 CTGCAG
PvuII 367 CAGCTG CAG^CTG
412 CAGCTG
SfcI 133 CTGCAG C^T[GA][CT]AG
508 CTGCAG
Tsp45I 523 GTCAC ^GT[GC]AC
532 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeII MboI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PflMI Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI Van91I