Restriction map for
pF1KB9934
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbvCI
BcgI BclI BfrI BlnI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI Eam1105I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MseI MunI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 1222 GGCGCC G[GA]^CG[CT]C
AflIII 327 ACGTGT A^C[GA][CT]GT
AgeI 642 ACCGGT A^CCGGT
AocI 169 CCTGAGG CC^T[ACGT]AGG
Aor51HI 266 AGCGCT AGC^GCT
ApaI 151 GGGCCC GGGCC^C
AspI 1046 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1222 GGCGCC GGCGC^C
BbsI 43 GAAGAC GAAGAC(2/6)
BciVI 1814 GTATCC GTATCC(6/5)
BfaI 1114 CTAG C^TAG
BglII 1127 AGATCT A^GATCT
BpuAI 43 GAAGAC GAAGAC(2/6)
BsaHI 1222 GGCGCC G[GA]^CG[CT]C
BsmBI 1720 GAGACG CGTCTC(1/5)
BsrDI 1245 CATTGC GCAATG(2/0)
BssSI 88 CACGAG CACGAG(-5/-1)
BstXI 1688 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 169 CCTGAGG CC^T[ACGT]AGG
DraIII 469 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1768 GACGGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 266 AGCGCT AGC^GCT
Eco81I 169 CCTGAGG CC^T[ACGT]AGG
EcoRV 1918 GATATC GAT^ATC
Hin1I 1222 GGCGCC G[GA]^CG[CT]C
HincII 493 GTCAAC GT[CT]^[GA]AC
HindII 493 GTCAAC GT[CT]^[GA]AC
KasI 1222 GGCGCC G^GCGCC
KspI 293 CCGCGG CCGC^GG
MaeI 1114 CTAG C^TAG
MslI 52 CACAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 793 GCCGGC GCC^GGC
NarI 1222 GGCGCC GG^CGCC
NcoI 447 CCATGG C^CATGG
NgoMIV 793 GCCGGC G^CCGGC
NotI 103 GCGGCCGC GC^GGCCGC
PflFI 1046 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 642 ACCGGT A^CCGGT
SacII 293 CCGCGG CCGC^GG
SbfI 672 CCTGCAGG CCTGCA^GG
SexAI 455 ACCAGGT A^CC[AT]GGT
SfoI 1222 GGCGCC GGC^GCC
SmaI 1297 CCCGGG CCC^GGG
Sse8387I 672 CCTGCAGG CCTGCA^GG
Tth111I 1046 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1297 CCCGGG C^CCGGG
XmaI 1297 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 294 CGCG CG^CG
1644 CGCG
AccIII 1343 TCCGGA T^CCGGA
1817 TCCGGA
AfaI 1390 GTAC GT^AC
1641 GTAC
AvaI 562 CCCGAG C^[CT]CG[GA]G
1297 CCCGGG
AvaII 297 GGACC G^G[AT]CC
1051 GGTCC
BbrPI 472 CACGTG CAC^GTG
1903 CACGTG
BglI 94 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
793 GCCGGCCTGGC
BlpI 517 GCTGAGC GC^T[ACGT]AGC
948 GCTCAGC
Bpu1102I 517 GCTGAGC GC^T[ACGT]AGC
948 GCTCAGC
BseAI 1343 TCCGGA T^CCGGA
1817 TCCGGA
BsiEI 104 CGGCCG CG[GA][CT]^CG
320 CGGCCG
BsoBI 562 CCCGAG C^[CT]CG[GA]G
1297 CCCGGG
BspEI 1343 TCCGGA T^CCGGA
1817 TCCGGA
BspMI 603 GCAGGT ACCTGC(4/8)
1198 GCAGGT
BstUI 294 CGCG CG^CG
1644 CGCG
BstYI 485 GGATCT [GA]^GATC[CT]
1127 AGATCT
CelII 517 GCTGAGC GC^T[ACGT]AGC
948 GCTCAGC
EagI 104 CGGCCG C^GGCCG
320 CGGCCG
EarI 225 GAAGAG CTCTTC(1/4)
1446 GAAGAG
EclXI 104 CGGCCG C^GGCCG
320 CGGCCG
Eco52I 104 CGGCCG C^GGCCG
320 CGGCCG
HgaI 175 GACGC GACGC(5/10)
1156 GACGC
Ksp632I 225 GAAGAG CTCTTC(1/4)
1446 GAAGAG
MflI 485 GGATCT [GA]^GATC[CT]
1127 AGATCT
MroI 1343 TCCGGA T^CCGGA
1817 TCCGGA
MvnI 294 CGCG CG^CG
1644 CGCG
PflMI 378 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
600 CCAGCAGGTGG
PleI 585 GACTC GAGTC(4/5)
1374 GAGTC
PmaCI 472 CACGTG CAC^GTG
1903 CACGTG
PmlI 472 CACGTG CAC^GTG
1903 CACGTG
RsaI 1390 GTAC GT^AC
1641 GTAC
SapI 225 GAAGAGC GCTCTTC(1/4)
1446 GAAGAGC
SfaNI 718 GATGC GCATC(5/9)
931 GATGC
StuI 14 AGGCCT AGG^CCT
1742 AGGCCT
TfiI 1169 GATTC G^A[AT]TC
1830 GATTC
Van91I 378 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
600 CCAGCAGGTGG
XhoII 485 GGATCT [GA]^GATC[CT]
1127 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII SacI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI