Restriction map for
pF1KB9936
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# NO CUT ENZYMES ( 89 enzymes) #
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AccI AclI AflII AgeI AocI
Aor51HI AscI AseI AsnI Asp700I
AviII AvrII BamHI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmI BspLU11I BsrDI BssHII Bst1107I
BstEII BstPI BstYI BstZ17I Bsu36I
CelII CpoI DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII MfeI MflI
MslI MunI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI SexAI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AatII 1756 GACGTC GACGT^C
Acc65I 1248 GGTACC G^GTACC
AccIII 1886 TCCGGA T^CCGGA
AcsI 25 AAATTC [GA]^AATT[CT]
AflIII 1457 ACGCGT A^C[GA][CT]GT
AhdI 1024 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 523 GTGCAC G^TGCAC
ApaI 159 GGGCCC GGGCC^C
ApaLI 523 GTGCAC G^TGCAC
ApoI 25 AAATTC [GA]^AATT[CT]
Asp718I 1248 GGTACC G^GTACC
AspEI 1024 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 624 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1287 TGGCCA TGG^CCA
BbsI 621 GAAGAC GAAGAC(2/6)
BciVI 1651 GGATAC GTATCC(6/5)
BfaI 1346 CTAG C^TAG
BpuAI 621 GAAGAC GAAGAC(2/6)
BsaI 568 GAGACC GGTCTC(1/5)
BsaWI 1886 TCCGGA [AT]^CCGG[AT]
BseAI 1886 TCCGGA T^CCGGA
BsmBI 70 GAGACG CGTCTC(1/5)
Bsp1407I 1559 TGTACA T^GTACA
BspDI 1296 ATCGAT AT^CGAT
BspEI 1886 TCCGGA T^CCGGA
BspHI 92 TCATGA T^CATGA
BsrBI 769 CCGCTC CCGCTC(-3/-3)
BsrGI 1559 TGTACA T^GTACA
BssSI 1214 CTCGTG CACGAG(-5/-1)
BstAPI 1041 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 9 TTCGAA TT^CGAA
ClaI 1296 ATCGAT AT^CGAT
DrdI 1695 GACAGGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1024 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 565 GAAGAG CTCTTC(1/4)
HincII 546 GTTAAC GT[CT]^[GA]AC
HindII 546 GTTAAC GT[CT]^[GA]AC
HpaI 546 GTTAAC GTT^AAC
KpnI 1248 GGTACC GGTAC^C
Ksp632I 565 GAAGAG CTCTTC(1/4)
MaeI 1346 CTAG C^TAG
MluI 1457 ACGCGT A^CGCGT
MluNI 1287 TGGCCA TGG^CCA
MroI 1886 TCCGGA T^CCGGA
MscI 1287 TGGCCA TGG^CCA
MseI 547 TTAA T^TAA
NspI 1832 ACATGC [GA]CATG^[CT]
NspV 9 TTCGAA TT^CGAA
PflFI 624 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1330 CTGCAG CTGCA^G
RcaI 92 TCATGA T^CATGA
SacI 1413 GAGCTC GAGCT^C
ScaI 176 AGTACT AGT^ACT
SfiI 1482 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 9 TTCGAA TT^CGAA
SmaI 1566 CCCGGG CCC^GGG
SmlI 1798 CTCAAG C^T[CT][GA]AG
SspBI 1559 TGTACA T^GTACA
Tru9I 547 TTAA T^TAA
Tth111I 624 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 207 CCAGATCGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1566 CCCGGG C^CCGGG
XmaI 1566 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AlwI 705 GATCC GGATC(4/5)
1304 GGATC
BsaBI 1299 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1305 GATCGACATC
BspMI 149 ACCTGC ACCTGC(4/8)
230 ACCTGC
BstXI 174 CCAGTACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1733 CCAGCTCTCTGG
KspI 19 CCGCGG CCGC^GG
422 CCGCGG
MamI 1299 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1305 GATCGACATC
PflMI 291 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1506 CCACCAGCTGG
SacII 19 CCGCGG CCGC^GG
422 CCGCGG
TfiI 496 GAATC G^A[AT]TC
659 GAATC
Tsp509I 26 AATT ^AATT
448 AATT
Van91I 291 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1506 CCACCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TseI Tsp45I TspRI TthHB8I