Restriction map for
pF1KB9973
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BcgI BciVI BclI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeII MamI MfeI
MluI MroI MslI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 208 GTAGAC GT^[AC][GT]AC
AccII 6 CGCG CG^CG
AcyI 13 GGCGCC G[GA]^CG[CT]C
AflII 244 CTTAAG C^TTAAG
ApaI 1110 GGGCCC GGGCC^C
Asp700I 492 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1128 TGGCCA TGG^CCA
BbeI 13 GGCGCC GGCGC^C
BbvCI 1236 CCTCAGC CCTCAGC(-5/-2)
BfaI 593 CTAG C^TAG
BfrI 244 CTTAAG C^TTAAG
BlpI 1261 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1261 GCTCAGC GC^T[ACGT]AGC
BsaHI 13 GGCGCC G[GA]^CG[CT]C
BsmI 529 GAATGC GAATGC(1/-1)
BsrBI 1589 GAGCGG CCGCTC(-3/-3)
BsrDI 892 GCAATG GCAATG(2/0)
BsrFI 1074 GCCGGC [GA]^CCGG[CT]
BssSI 183 CTCGTG CACGAG(-5/-1)
BstEII 549 GGTGACC G^GT[ACGT]ACC
BstPI 549 GGTGACC G^GT[ACGT]ACC
BstUI 6 CGCG CG^CG
BstXI 120 CCACATGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1261 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1074 GCCGGC [GA]^CCGG[CT]
DraIII 724 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 641 CCATGG C^C[GA][CT]GG
EcoO65I 549 GGTGACC G^GT[ACGT]ACC
HgaI 401 GCGTC GACGC(5/10)
Hin1I 13 GGCGCC G[GA]^CG[CT]C
KasI 13 GGCGCC G^GCGCC
MaeI 593 CTAG C^TAG
MluNI 1128 TGGCCA TGG^CCA
MscI 1128 TGGCCA TGG^CCA
MvnI 6 CGCG CG^CG
NaeI 1074 GCCGGC GCC^GGC
NarI 13 GGCGCC GG^CGCC
NcoI 641 CCATGG C^CATGG
NgoMIV 1074 GCCGGC G^CCGGC
NspI 995 GCATGC [GA]CATG^[CT]
PpuMI 908 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 331 CAGCTG CAG^CTG
ScaI 110 AGTACT AGT^ACT
SfoI 13 GGCGCC GGC^GCC
SmaI 865 CCCGGG CCC^GGG
SmlI 244 CTTAAG C^T[CT][GA]AG
SphI 995 GCATGC GCATG^C
SspI 493 AATATT AAT^ATT
XmaCI 865 CCCGGG C^CCGGG
XmaI 865 CCCGGG C^CCGGG
XmnI 492 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 765 GAGCAC G[AT]GC[AT]^C
1332 GAGCAC
AvaI 865 CCCGGG C^[CT]CG[GA]G
1290 CTCGGG
AvaII 909 GGTCC G^G[AT]CC
1372 GGACC
BamHI 732 GGATCC G^GATCC
1459 GGATCC
BanI 13 GGCGCC G^G[CT][GA]CC
1503 GGTGCC
BbsI 1155 GAAGAC GAAGAC(2/6)
1489 GTCTTC
BglI 646 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1226 GCCCCCAGGGC
BpuAI 1155 GAAGAC GAAGAC(2/6)
1489 GTCTTC
BsgI 721 CTGCAC GTGCAG(16/14)
328 GTGCAG
BsiHKAI 765 GAGCAC G[AT]GC[AT]^C
1332 GAGCAC
BsmFI 958 GGGAC GGGAC(10/14)
910 GTCCC
BsoBI 865 CCCGGG C^[CT]CG[GA]G
1290 CTCGGG
EarI 357 GAAGAG CTCTTC(1/4)
382 GAAGAG
HaeII 13 GGCGCC [GA]GCGC^[CT]
23 GGCGCT
Ksp632I 357 GAAGAG CTCTTC(1/4)
382 GAAGAG
SapI 357 GAAGAGC GCTCTTC(1/4)
382 GAAGAGC
SfaNI 1036 GCATC GCATC(5/9)
1054 GCATC
StuI 395 AGGCCT AGG^CCT
1539 AGGCCT
Tsp45I 550 GTGAC ^GT[GC]AC
599 GTGAC
Tsp509I 192 AATT ^AATT
904 AATT
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TfiI
Tru9I TseI TspRI Van91I XcmI
XhoII