Restriction map for
pF1KB5826
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BpuAI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MfeI MluI MroI
MslI MunI MvnI NarI NciI
NdeI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AciI 1326 GCGG CCGC(-3/-1)
AfaI 402 GTAC GT^AC
AflIII 850 ACATGT A^C[GA][CT]GT
Aor51HI 167 AGCGCT AGC^GCT
AspHI 679 GAGCTC G[AT]GC[AT]^C
AviII 1088 TGCGCA TGC^GCA
BalI 93 TGGCCA TGG^CCA
BanII 679 GAGCTC G[GA]GC[CT]^C
BbvCI 843 GCTGAGG CCTCAGC(-5/-2)
BglI 646 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 713 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 713 GCTCAGC GC^T[ACGT]AGC
BsaBI 976 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1251 GGTCTC GGTCTC(1/5)
BsiHKAI 679 GAGCTC G[AT]GC[AT]^C
BsmI 112 GAATGC GAATGC(1/-1)
BspLU11I 850 ACATGT A^CATGT
BsrFI 161 GCCGGC [GA]^CCGG[CT]
BstAPI 1101 GCACTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 606 GGATG GGATG(2/0)
CelII 713 GCTCAGC GC^T[ACGT]AGC
Cfr10I 161 GCCGGC [GA]^CCGG[CT]
DrdI 1063 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 93 TGGCCA [CT]^GGCC[GA]
EarI 964 GAAGAG CTCTTC(1/4)
Eco47III 167 AGCGCT AGC^GCT
EcoT22I 114 ATGCAT ATGCA^T
FokI 606 GGATG GGATG(9/13)
FspI 1088 TGCGCA TGC^GCA
HaeII 167 AGCGCT [GA]GCGC^[CT]
HgaI 146 GCGTC GACGC(5/10)
Ksp632I 964 GAAGAG CTCTTC(1/4)
MamI 976 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MseI 227 TTAA T^TAA
NaeI 161 GCCGGC GCC^GGC
NgoMIV 161 GCCGGC G^CCGGC
NheI 204 GCTAGC G^CTAGC
NsiI 114 ATGCAT ATGCA^T
RsaI 402 GTAC GT^AC
SacI 679 GAGCTC GAGCT^C
SfiI 645 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 1396 AATATT AAT^ATT
StuI 1055 AGGCCT AGG^CCT
Tru9I 227 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BamHI 873 GGATCC G^GATCC
1411 GGATCC
BseRI 34 GAGGAG GAGGAG(10/8)
385 GAGGAG
BsmFI 1061 GGGAC GGGAC(10/14)
376 GTCCC
BstYI 873 GGATCC [GA]^GATC[CT]
1411 GGATCC
CfoI 168 GCGC GCG^C
1089 GCGC
EcoT14I 371 CCATGG C^C[AT][AT]GG
1275 CCATGG
HapII 162 CCGG C^CGG
189 CCGG
HhaI 168 GCGC GCG^C
1089 GCGC
HinP1I 168 GCGC G^CGC
1089 GCGC
HpaII 162 CCGG C^CGG
189 CCGG
MflI 873 GGATCC [GA]^GATC[CT]
1411 GGATCC
MspA1I 586 CAGCTG C[AC]G^C[GT]G
619 CAGCTG
MspI 162 CCGG C^CGG
189 CCGG
NcoI 371 CCATGG C^CATGG
1275 CCATGG
NspI 43 ACATGC [GA]CATG^[CT]
850 ACATGT
PflMI 366 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
887 CCAATCCGTGG
PvuII 586 CAGCTG CAG^CTG
619 CAGCTG
SmlI 178 CTCAAG C^T[CT][GA]AG
382 CTTGAG
StyI 371 CCATGG C^C[AT][AT]GG
1275 CCATGG
Van91I 366 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
887 CCAATCCGTGG
XhoII 873 GGATCC [GA]^GATC[CT]
1411 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AluI AlwI AlwNI AvaII BanI
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I