##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BbeI        BbrPI       BbsI        BbvI        
BcgI        BciVI       BfrI        BglI        BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        Fnu4HI      FokI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MunI        MvnI        MwoI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AspHI         331  GAGCTC               G[AT]GC[AT]^C
AvaII         224  GGTCC                G^G[AT]CC
BamHI         242  GGATCC               G^GATCC
BbvCI         227  CCTCAGC              CCTCAGC(-5/-2)
BclI          342  TGATCA               T^GATCA
BcnI          186  CCGGG                CC^[GC]GG
BfaI          262  CTAG                 C^TAG
BglII          28  AGATCT               A^GATCT
BlpI          333  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      333  GCTCAGC              GC^T[ACGT]AGC
BsiHKAI       331  GAGCTC               G[AT]GC[AT]^C
BsrI          315  ACTGG                ACTGG(1/-1)
Cac8I          19  GCGAGC               GC[ACGT]^[ACGT]GC
CelII         333  GCTCAGC              GC^T[ACGT]AGC
DraII          65  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I       65  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       255  CCTTGG               C^C[AT][AT]GG
FbaI          342  TGATCA               T^GATCA
HapII         186  CCGG                 C^CGG
HindIII        38  AAGCTT               A^AGCTT
HpaII         186  CCGG                 C^CGG
HphI           96  GGTGA                GGTGA(8/7)
MaeI          262  CTAG                 C^TAG
MaeIII        312  GTCAC                ^GT[ACGT]AC
MboII           7  TCTTC                GAAGA(8/7)
MspI          186  CCGG                 C^CGG
NciI          186  CCGGG                CC^[GC]GG
PstI           45  CTGCAG               CTGCA^G
SacI          331  GAGCTC               GAGCT^C
SfaNI          99  GATGC                GCATC(5/9)
SfcI           45  CTGCAG               C^T[GA][CT]AG
SmlI           35  CTCAAG               C^T[CT][GA]AG
StyI          255  CCTTGG               C^C[AT][AT]GG
Tsp45I        312  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AlwNI          64  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              230  CAGCAACTG           
BanI          163  GGTGCC               G^G[CT][GA]CC
              182  GGTGCC              
BanII         116  GGGCTC               G[GA]GC[CT]^C
              331  GAGCTC              
BpmI          270  CTCCAG               CTGGAG(16/14)
              287  CTGGAG              
BsaI           80  GAGACC               GGTCTC(1/5)
              265  GGTCTC              
StuI          252  AGGCCT               AGG^CCT
              274  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AluI        AlwI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstNI       
BstYI       Cfr13I      DdeI        DpnII       EcoRII      
HaeIII      MboI        MflI        MnlI        MvaI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
TspRI       XhoII