Restriction map for
pF1KB5835
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AlwNI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
AvrII BanI BbeI BbsI BbvCI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DrdI EagI EarI EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 1205 CGCG CG^CG
AcsI 1133 GAATTC [GA]^AATT[CT]
AflII 1267 CTTAAG C^TTAAG
AflIII 384 ACATGT A^C[GA][CT]GT
AhdI 559 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 79 GTGCAC G^TGCAC
ApaLI 79 GTGCAC G^TGCAC
ApoI 1133 GAATTC [GA]^AATT[CT]
Asp700I 138 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 559 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1460 CTCGAG C^[CT]CG[GA]G
AvaII 1248 GGACC G^G[AT]CC
BalI 1199 TGGCCA TGG^CCA
BamHI 1575 GGATCC G^GATCC
BbrPI 553 CACGTG CAC^GTG
BcgI 796 CGACTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 579 GGATAC GTATCC(6/5)
BcnI 1111 CCGGG CC^[GC]GG
BfrI 1267 CTTAAG C^TTAAG
BglI 1374 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 553 CACGTG [CT]AC^GT[GA]
BsgI 111 CTGCAC GTGCAG(16/14)
BsmBI 566 CGTCTC CGTCTC(1/5)
BsoBI 1460 CTCGAG C^[CT]CG[GA]G
BspLU11I 384 ACATGT A^CATGT
BspMI 271 ACCTGC ACCTGC(4/8)
BstUI 1205 CGCG CG^CG
BstYI 1575 GGATCC [GA]^GATC[CT]
DraI 936 TTTAAA TTT^AAA
DraIII 320 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 64 CCACGG C^C[GA][CT]GG
EaeI 1199 TGGCCA [CT]^GGCC[GA]
Eam1105I 559 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1133 GAATTC G^AATTC
EcoRV 643 GATATC GAT^ATC
EcoT14I 1097 CCAAGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
HapII 1111 CCGG C^CGG
HgaI 565 GCGTC GACGC(5/10)
HpaII 1111 CCGG C^CGG
MaeII 554 ACGT A^CGT
MflI 1575 GGATCC [GA]^GATC[CT]
MluNI 1199 TGGCCA TGG^CCA
MscI 1199 TGGCCA TGG^CCA
MspI 1111 CCGG C^CGG
MvnI 1205 CGCG CG^CG
NciI 1111 CCGGG CC^[GC]GG
NdeI 968 CATATG CA^TATG
NsiI 1 ATGCAT ATGCA^T
PaeR7I 1460 CTCGAG C^TCGAG
PmaCI 553 CACGTG CAC^GTG
PmlI 553 CACGTG CAC^GTG
PpuMI 1247 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1488 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SphI 1283 GCATGC GCATG^C
StyI 1097 CCAAGG C^C[AT][AT]GG
TaqI 1461 TCGA T^CGA
TfiI 542 GAATC G^A[AT]TC
TthHB8I 1461 TCGA T^CGA
XhoI 1460 CTCGAG C^TCGAG
XhoII 1575 GGATCC [GA]^GATC[CT]
XmnI 138 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 205 GTAC GT^AC
1344 GTAC
AlwI 1576 GATCC GGATC(4/5)
1575 GGATC
AocI 1243 CCTGAGG CC^T[ACGT]AGG
1375 CCTCAGG
BclI 926 TGATCA T^GATCA
1157 TGATCA
BsmFI 183 GTCCC GGGAC(10/14)
1495 GTCCC
BsrDI 84 CATTGC GCAATG(2/0)
587 GCAATG
Bsu36I 1243 CCTGAGG CC^T[ACGT]AGG
1375 CCTCAGG
Eco81I 1243 CCTGAGG CC^T[ACGT]AGG
1375 CCTCAGG
EcoNI 120 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1492 CCTGTCCCAGG
FauI 947 GCGGG CCCGC(4/6)
1206 GCGGG
FbaI 926 TGATCA T^GATCA
1157 TGATCA
MseI 937 TTAA T^TAA
1268 TTAA
MslI 682 CATAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1045 CATTTCCATG
MspA1I 827 CAGCTG C[AC]G^C[GT]G
1009 CAGCTG
PstI 133 CTGCAG CTGCA^G
156 CTGCAG
PvuII 827 CAGCTG CAG^CTG
1009 CAGCTG
RsaI 205 GTAC GT^AC
1344 GTAC
SacI 1409 GAGCTC GAGCT^C
1457 GAGCTC
Tru9I 937 TTAA T^TAA
1268 TTAA
Tsp509I 365 AATT ^AATT
1134 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI ApaI AspHI BanII
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TseI
Tsp45I TspRI