Restriction map for
pF1KB5733
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI AspEI AspI
AvrII BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI HincII HindII HindIII
HpaI MfeI MluI MroI MunI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI TfiI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 365 GGTACC G^GTACC
AcyI 1294 GGCGCC G[GA]^CG[CT]C
Alw44I 1077 GTGCAC G^TGCAC
ApaI 762 GGGCCC GGGCC^C
ApaLI 1077 GTGCAC G^TGCAC
Asp700I 81 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 365 GGTACC G^GTACC
BamHI 1623 GGATCC G^GATCC
BbeI 1294 GGCGCC GGCGC^C
BbrPI 96 CACGTG CAC^GTG
BglII 176 AGATCT A^GATCT
BsaAI 96 CACGTG [CT]AC^GT[GA]
BsaBI 1618 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1294 GGCGCC G[GA]^CG[CT]C
BsgI 1813 CTGCAC GTGCAG(16/14)
Bsp1407I 2246 TGTACA T^GTACA
BspHI 1598 TCATGA T^CATGA
BsrBI 1533 CCGCTC CCGCTC(-3/-3)
BsrDI 1131 CATTGC GCAATG(2/0)
BsrGI 2246 TGTACA T^GTACA
Hin1I 1294 GGCGCC G[GA]^CG[CT]C
KasI 1294 GGCGCC G^GCGCC
KpnI 365 GGTACC GGTAC^C
KspI 244 CCGCGG CCGC^GG
MamI 1618 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1583 TTAA T^TAA
NarI 1294 GGCGCC GG^CGCC
NdeI 1268 CATATG CA^TATG
NheI 57 GCTAGC G^CTAGC
PaeR7I 1393 CTCGAG C^TCGAG
PmaCI 96 CACGTG CAC^GTG
PmlI 96 CACGTG CAC^GTG
RcaI 1598 TCATGA T^CATGA
SacII 244 CCGCGG CCGC^GG
SapI 1821 GAAGAGC GCTCTTC(1/4)
SfoI 1294 GGCGCC GGC^GCC
SphI 674 GCATGC GCATG^C
SspBI 2246 TGTACA T^GTACA
Tru9I 1583 TTAA T^TAA
XhoI 1393 CTCGAG C^TCGAG
XmnI 81 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AflIII 95 ACACGT A^C[GA][CT]GT
323 ACGTGT
AocI 1723 CCTGAGG CC^T[ACGT]AGG
2140 CCTGAGG
AspHI 1077 GTGCAC G[AT]GC[AT]^C
1453 GAGCAC
AviII 107 TGCGCA TGC^GCA
671 TGCGCA
BcgI 1058 CGACTGACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2067 GCAGCCACCTCG
BfaI 58 CTAG C^TAG
1645 CTAG
BsaI 1900 GAGACC GGTCTC(1/5)
444 GGTCTC
BsiHKAI 1077 GTGCAC G[AT]GC[AT]^C
1453 GAGCAC
BspMI 2135 ACCTGC ACCTGC(4/8)
294 GCAGGT
BstYI 176 AGATCT [GA]^GATC[CT]
1623 GGATCC
Bsu36I 1723 CCTGAGG CC^T[ACGT]AGG
2140 CCTGAGG
DrdI 216 GACGAAGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1764 GACCCTCACGTC
EarI 1821 GAAGAG CTCTTC(1/4)
2326 GAAGAG
Eco81I 1723 CCTGAGG CC^T[ACGT]AGG
2140 CCTGAGG
EcoNI 804 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2136 CCTGCCTGAGG
FseI 1958 GGCCGGCC GGCCGG^CC
2151 GGCCGGCC
FspI 107 TGCGCA TGC^GCA
671 TGCGCA
Ksp632I 1821 GAAGAG CTCTTC(1/4)
2326 GAAGAG
MaeI 58 CTAG C^TAG
1645 CTAG
MflI 176 AGATCT [GA]^GATC[CT]
1623 GGATCC
PflMI 92 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1223 CCAACGACTGG
PstI 259 CTGCAG CTGCA^G
2206 CTGCAG
SexAI 1184 ACCTGGT A^CC[AT]GGT
1511 ACCTGGT
SmlI 272 CTCAAG C^T[CT][GA]AG
1393 CTCGAG
Tsp509I 496 AATT ^AATT
1318 AATT
Van91I 92 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1223 CCAACGACTGG
XcmI 1235 CCACCAATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1298 CCATGGTGGCCCTGG
XhoII 176 AGATCT [GA]^GATC[CT]
1623 GGATCC
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I