Restriction map for
pF1KB5849
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AvrII
BamHI BbeI BbrPI BbsI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FseI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MroI MseI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AocI 430 CCTGAGG CC^T[ACGT]AGG
Aor51HI 635 AGCGCT AGC^GCT
AviII 368 TGCGCA TGC^GCA
BalI 44 TGGCCA TGG^CCA
BanI 568 GGCACC G^G[CT][GA]CC
BbvCI 253 GCTGAGG CCTCAGC(-5/-2)
BclI 287 TGATCA T^GATCA
BsaWI 302 ACCGGA [AT]^CCGG[AT]
BsiEI 300 CGACCG CG[GA][CT]^CG
BsmI 397 GAATGC GAATGC(1/-1)
Bsp1407I 479 TGTACA T^GTACA
BsrGI 479 TGTACA T^GTACA
BstAPI 78 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 453 GGTAACC G^GT[ACGT]ACC
BstNI 362 CCTGG CC^[AT]GG
BstPI 453 GGTAACC G^GT[ACGT]ACC
Bsu36I 430 CCTGAGG CC^T[ACGT]AGG
EaeI 44 TGGCCA [CT]^GGCC[GA]
Eco47III 635 AGCGCT AGC^GCT
Eco81I 430 CCTGAGG CC^T[ACGT]AGG
EcoO65I 453 GGTAACC G^GT[ACGT]ACC
EcoRII 362 CCTGG ^CC[AT]GG
FbaI 287 TGATCA T^GATCA
FspI 368 TGCGCA TGC^GCA
HaeII 635 AGCGCT [GA]GCGC^[CT]
HgaI 114 GCGTC GACGC(5/10)
MluNI 44 TGGCCA TGG^CCA
MscI 44 TGGCCA TGG^CCA
MslI 279 CACATATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 362 CCTGG CC^[AT]GG
NdeI 281 CATATG CA^TATG
NspI 629 ACATGC [GA]CATG^[CT]
SmaI 504 CCCGGG CCC^GGG
SspBI 479 TGTACA T^GTACA
StuI 668 AGGCCT AGG^CCT
TaqI 299 TCGA T^CGA
Tsp45I 98 GTCAC ^GT[GC]AC
TthHB8I 299 TCGA T^CGA
XmaCI 504 CCCGGG C^CCGGG
XmaI 504 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 358 CGCG CG^CG
388 CGCG
AlwNI 79 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
176 CAGCCACTG
AvaI 504 CCCGGG C^[CT]CG[GA]G
595 CCCGAG
BpmI 37 CTGGAG CTGGAG(16/14)
88 CTGGAG
BseRI 228 GAGGAG GAGGAG(10/8)
439 GAGGAG
BsoBI 504 CCCGGG C^[CT]CG[GA]G
595 CCCGAG
BstUI 358 CGCG CG^CG
388 CGCG
BstYI 529 AGATCC [GA]^GATC[CT]
710 AGATCC
EarI 93 GAAGAG CTCTTC(1/4)
225 GAAGAG
HinfI 96 GAGTC G^A[ACGT]TC
468 GAGTC
Ksp632I 93 GAAGAG CTCTTC(1/4)
225 GAAGAG
MflI 529 AGATCC [GA]^GATC[CT]
710 AGATCC
MspA1I 214 CAGCGG C[AC]G^C[GT]G
424 CCGCTG
MvnI 358 CGCG CG^CG
388 CGCG
PleI 96 GAGTC GAGTC(4/5)
468 GAGTC
PstI 525 CTGCAG CTGCA^G
550 CTGCAG
SfcI 525 CTGCAG C^T[GA][CT]AG
550 CTGCAG
XhoII 529 AGATCC [GA]^GATC[CT]
710 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
Cac8I CfoI Cfr13I DdeI DpnII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MboII MnlI MspI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI TspRI