Restriction map for
pF1KB5736
##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BclI
BglII BlnI BlpI Bpu1102I BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DrdI Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI MluI MroI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SapI SbfI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 747 GACGTC GACGT^C
AccI 1779 GTCGAC GT^[AC][GT]AC
AcsI 209 AAATTT [GA]^AATT[CT]
AflII 697 CTTAAG C^TTAAG
Alw44I 1021 GTGCAC G^TGCAC
AlwNI 1298 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1075 CCTCAGG CC^T[ACGT]AGG
ApaLI 1021 GTGCAC G^TGCAC
ApoI 209 AAATTT [GA]^AATT[CT]
BamHI 1065 GGATCC G^GATCC
BbrPI 1393 CACGTG CAC^GTG
BbsI 1510 GTCTTC GAAGAC(2/6)
BbvCI 1008 GCTGAGG CCTCAGC(-5/-2)
BcgI 110 GCATCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 697 CTTAAG C^TTAAG
BglI 1877 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1510 GTCTTC GAAGAC(2/6)
BsaAI 1393 CACGTG [CT]AC^GT[GA]
BsaBI 1338 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2086 GTGCAG GTGCAG(16/14)
BsiEI 917 CGGCCG CG[GA][CT]^CG
BsrDI 857 GCAATG GCAATG(2/0)
BsrFI 707 ACCGGC [GA]^CCGG[CT]
BstEII 1275 GGTGACC G^GT[ACGT]ACC
BstPI 1275 GGTGACC G^GT[ACGT]ACC
Bsu36I 1075 CCTCAGG CC^T[ACGT]AGG
Cfr10I 707 ACCGGC [GA]^CCGG[CT]
DraIII 1784 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 917 CGGCCG C^GGCCG
EarI 67 GAAGAG CTCTTC(1/4)
EclXI 917 CGGCCG C^GGCCG
Eco52I 917 CGGCCG C^GGCCG
Eco81I 1075 CCTCAGG CC^T[ACGT]AGG
EcoNI 57 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1275 GGTGACC G^GT[ACGT]ACC
HindIII 2157 AAGCTT A^AGCTT
Ksp632I 67 GAAGAG CTCTTC(1/4)
MamI 1338 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1118 CAATTG C^AATTG
MseI 698 TTAA T^TAA
MunI 1118 CAATTG C^AATTG
PmaCI 1393 CACGTG CAC^GTG
PmlI 1393 CACGTG CAC^GTG
PstI 139 CTGCAG CTGCA^G
SacI 579 GAGCTC GAGCT^C
SalI 1779 GTCGAC G^TCGAC
ScaI 161 AGTACT AGT^ACT
SexAI 80 ACCTGGT A^CC[AT]GGT
SgrAI 706 CACCGGCG C[GA]^CCGG[CT]G
TaqI 1780 TCGA T^CGA
Tru9I 698 TTAA T^TAA
TthHB8I 1780 TCGA T^CGA
XcmI 528 CCACAGGACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 150 CGCG CG^CG
1149 CGCG
AhdI 1307 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1466 GACACACCGTC
AspEI 1307 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1466 GACACACCGTC
BbeI 154 GGCGCC GGCGC^C
330 GGCGCC
BfaI 1546 CTAG C^TAG
1919 CTAG
BsmBI 37 GAGACG CGTCTC(1/5)
869 GAGACG
BspMI 653 GCAGGT ACCTGC(4/8)
954 GCAGGT
BstUI 150 CGCG CG^CG
1149 CGCG
BstYI 89 AGATCC [GA]^GATC[CT]
1065 GGATCC
Eam1105I 1307 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1466 GACACACCGTC
KasI 154 GGCGCC G^GCGCC
330 GGCGCC
KspI 149 CCGCGG CCGC^GG
1148 CCGCGG
MaeI 1546 CTAG C^TAG
1919 CTAG
MflI 89 AGATCC [GA]^GATC[CT]
1065 GGATCC
MvnI 150 CGCG CG^CG
1149 CGCG
NarI 154 GGCGCC GG^CGCC
330 GGCGCC
NcoI 1286 CCATGG C^CATGG
2171 CCATGG
NspI 944 ACATGC [GA]CATG^[CT]
1325 GCATGT
SacII 149 CCGCGG CCGC^GG
1148 CCGCGG
SfcI 139 CTGCAG C^T[GA][CT]AG
1680 CTACAG
SfoI 154 GGCGCC GGC^GCC
330 GGCGCC
TfiI 435 GAATC G^A[AT]TC
772 GATTC
Tsp509I 210 AATT ^AATT
1119 AATT
XhoII 89 AGATCC [GA]^GATC[CT]
1065 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BciVI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrBI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StuI StyI TseI
Tsp45I TspRI Van91I