Restriction map for
pF1KB5737
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI ApoI AscI
AseI AsnI Asp700I AspEI AvrII
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I CelII CpoI
DraI DraIII Eam1105I EcoRI EcoRV
EcoT22I FbaI HincII HindII HpaI
KspI MamI MluI MroI NdeI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 1330 GGTACC G^GTACC
Alw44I 1618 GTGCAC G^TGCAC
Aor51HI 1247 AGCGCT AGC^GCT
ApaLI 1618 GTGCAC G^TGCAC
Asp718I 1330 GGTACC G^GTACC
AviII 425 TGCGCA TGC^GCA
BamHI 719 GGATCC G^GATCC
BsmBI 384 GAGACG CGTCTC(1/5)
BspDI 1219 ATCGAT AT^CGAT
BsrDI 1574 GCAATG GCAATG(2/0)
BssSI 514 CACGAG CACGAG(-5/-1)
BstAPI 1660 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1959 GGTCACC G^GT[ACGT]ACC
BstPI 1959 GGTCACC G^GT[ACGT]ACC
BstXI 1076 CCAAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1219 ATCGAT AT^CGAT
DrdI 1414 GACCGGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1247 AGCGCT AGC^GCT
EcoO65I 1959 GGTCACC G^GT[ACGT]ACC
FseI 1119 GGCCGGCC GGCCGG^CC
FspI 425 TGCGCA TGC^GCA
HindIII 673 AAGCTT A^AGCTT
KpnI 1330 GGTACC GGTAC^C
MfeI 190 CAATTG C^AATTG
MunI 190 CAATTG C^AATTG
NaeI 1120 GCCGGC GCC^GGC
NgoMIV 1120 GCCGGC G^CCGGC
SacI 173 GAGCTC GAGCT^C
SmlI 309 CTCAAG C^T[CT][GA]AG
SspI 652 AATATT AAT^ATT
Tsp509I 191 AATT ^AATT
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 580 GTCTAC GT^[AC][GT]AC
1213 GTCTAC
AocI 1950 CCTGAGG CC^T[ACGT]AGG
2029 CCTGAGG
BbsI 1062 GAAGAC GAAGAC(2/6)
1423 GTCTTC
BfaI 134 CTAG C^TAG
622 CTAG
BpuAI 1062 GAAGAC GAAGAC(2/6)
1423 GTCTTC
BsiEI 58 CGGCCG CG[GA][CT]^CG
121 CGGCCG
BspHI 452 TCATGA T^CATGA
1719 TCATGA
BspMI 584 ACCTGC ACCTGC(4/8)
642 GCAGGT
BsrFI 1120 GCCGGC [GA]^CCGG[CT]
1415 ACCGGC
BstYI 719 GGATCC [GA]^GATC[CT]
1682 AGATCC
Bsu36I 1950 CCTGAGG CC^T[ACGT]AGG
2029 CCTGAGG
Cfr10I 1120 GCCGGC [GA]^CCGG[CT]
1415 ACCGGC
EagI 58 CGGCCG C^GGCCG
121 CGGCCG
EclXI 58 CGGCCG C^GGCCG
121 CGGCCG
Eco52I 58 CGGCCG C^GGCCG
121 CGGCCG
Eco81I 1950 CCTGAGG CC^T[ACGT]AGG
2029 CCTGAGG
MaeI 134 CTAG C^TAG
622 CTAG
MaeIII 1927 GTCAC ^GT[ACGT]AC
1960 GTCAC
MflI 719 GGATCC [GA]^GATC[CT]
1682 AGATCC
NcoI 1979 CCATGG C^CATGG
2253 CCATGG
NheI 133 GCTAGC G^CTAGC
621 GCTAGC
PvuII 683 CAGCTG CAG^CTG
1861 CAGCTG
RcaI 452 TCATGA T^CATGA
1719 TCATGA
Tsp45I 1927 GTCAC ^GT[GC]AC
1960 GTCAC
XcmI 73 CCAGGCCCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
262 CCAGTCAGACTCTGG
XhoII 719 GGATCC [GA]^GATC[CT]
1682 AGATCC
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI TaqI TfiI
Tru9I TseI TspRI Tth111I TthHB8I
Van91I XmaCI XmaI