Restriction map for
pF1KB5738
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I AspHI AspI
AviII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BlpI BmyI BpmI Bpu1102I
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
Bsp1286I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KspI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 447 GTCTAC GT^[AC][GT]AC
AciI 4 GCGG CCGC(-3/-1)
AflIII 603 ACATGT A^C[GA][CT]GT
AhdI 1054 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1174 CCTCAGG CC^T[ACGT]AGG
AseI 381 ATTAAT AT^TAAT
AsnI 381 ATTAAT AT^TAAT
AspEI 1054 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 471 CCTAGG C^CTAGG
BciVI 409 GTATCC GTATCC(6/5)
BglII 630 AGATCT A^GATCT
BlnI 471 CCTAGG C^CTAGG
BsaAI 1795 TACGTA [CT]AC^GT[GA]
BsaWI 783 ACCGGA [AT]^CCGG[AT]
BspLU11I 603 ACATGT A^CATGT
BstEII 1179 GGTTACC G^GT[ACGT]ACC
BstPI 1179 GGTTACC G^GT[ACGT]ACC
BstXI 1444 CCAGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1174 CCTCAGG CC^T[ACGT]AGG
Cac8I 456 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 2164 GCGC GCG^C
DraII 1593 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1054 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1174 CCTCAGG CC^T[ACGT]AGG
EcoNI 2151 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1593 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1179 GGTTACC G^GT[ACGT]ACC
EcoRI 553 GAATTC G^AATTC
HhaI 2164 GCGC GCG^C
HinP1I 2164 GCGC G^CGC
MaeII 1796 ACGT A^CGT
MfeI 308 CAATTG C^AATTG
MslI 397 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 2203 CAGCTG C[AC]G^C[GT]G
MunI 308 CAATTG C^AATTG
PflMI 751 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1593 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 381 ATTAAT AT^TAAT
PstI 134 CTGCAG CTGCA^G
PvuII 2203 CAGCTG CAG^CTG
SapI 1820 GAAGAGC GCTCTTC(1/4)
ScaI 1571 AGTACT AGT^ACT
SexAI 1428 ACCTGGT A^CC[AT]GGT
SnaBI 1795 TACGTA TAC^GTA
StuI 458 AGGCCT AGG^CCT
Van91I 751 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1880 GGTACC G^GTACC
2034 GGTACC
AlwNI 1313 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
1359 CAGACTCTG
Asp718I 1880 GGTACC G^GTACC
2034 GGTACC
AvaI 884 CCCGGG C^[CT]CG[GA]G
986 CCCGGG
BsmFI 600 GGGAC GGGAC(10/14)
1336 GTCCC
BsmI 702 GAATGC GAATGC(1/-1)
1230 GCATTC
BsoBI 884 CCCGGG C^[CT]CG[GA]G
986 CCCGGG
Bsp1407I 1320 TGTACA T^GTACA
1404 TGTACA
BspHI 417 TCATGA T^CATGA
1653 TCATGA
BsrGI 1320 TGTACA T^GTACA
1404 TGTACA
BstYI 295 GGATCT [GA]^GATC[CT]
630 AGATCT
DsaI 1743 CCACGG C^C[GA][CT]GG
1899 CCGTGG
EcoRV 79 GATATC GAT^ATC
1738 GATATC
EcoT22I 562 ATGCAT ATGCA^T
1267 ATGCAT
HincII 1062 GTCAAC GT[CT]^[GA]AC
1285 GTTGAC
HindII 1062 GTCAAC GT[CT]^[GA]AC
1285 GTTGAC
KpnI 1880 GGTACC GGTAC^C
2034 GGTACC
MflI 295 GGATCT [GA]^GATC[CT]
630 AGATCT
NsiI 562 ATGCAT ATGCA^T
1267 ATGCAT
NspI 603 ACATGT [GA]CATG^[CT]
1407 ACATGC
RcaI 417 TCATGA T^CATGA
1653 TCATGA
SfcI 134 CTGCAG C^T[GA][CT]AG
1536 CTGTAG
SmaI 884 CCCGGG CCC^GGG
986 CCCGGG
SspBI 1320 TGTACA T^GTACA
1404 TGTACA
TaqI 1238 TCGA T^CGA
1803 TCGA
TspRI 257 CAGTG CA[GC]TG[ACGT][ACGT]^
2039 CAGTG
TthHB8I 1238 TCGA T^CGA
1803 TCGA
XhoII 295 GGATCT [GA]^GATC[CT]
630 AGATCT
XmaCI 884 CCCGGG C^CCGGG
986 CCCGGG
XmaI 884 CCCGGG C^CCGGG
986 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BanI BbsI BbvI BcnI
BfaI BpuAI BsaJI BseRI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I