Restriction map for
pF1KB5740
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# NO CUT ENZYMES ( 107 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI AhdI ApoI AseI AsnI
Asp700I AspEI AspI AvrII BbeI
BbrPI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI MaeI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1630 GACGTC GACGT^C
AflIII 1778 ACACGT A^C[GA][CT]GT
Alw44I 417 GTGCAC G^TGCAC
AocI 702 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2144 AGCGCT AGC^GCT
ApaLI 417 GTGCAC G^TGCAC
AscI 598 GGCGCGCC GG^CGCGCC
AviII 230 TGCGCA TGC^GCA
BalI 1433 TGGCCA TGG^CCA
BamHI 1030 GGATCC G^GATCC
BcgI 1100 GCACCACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 432 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 432 GCTGAGC GC^T[ACGT]AGC
BsaBI 1642 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 912 ATCGAT AT^CGAT
BssSI 1464 CTCGTG CACGAG(-5/-1)
Bsu36I 702 CCTGAGG CC^T[ACGT]AGG
CelII 432 GCTGAGC GC^T[ACGT]AGC
ClaI 912 ATCGAT AT^CGAT
EarI 79 CTCTTC CTCTTC(1/4)
Eco47III 2144 AGCGCT AGC^GCT
Eco81I 702 CCTGAGG CC^T[ACGT]AGG
EcoRV 1408 GATATC GAT^ATC
FspI 230 TGCGCA TGC^GCA
Ksp632I 79 CTCTTC CTCTTC(1/4)
KspI 158 CCGCGG CCGC^GG
MamI 1642 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1433 TGGCCA TGG^CCA
MscI 1433 TGGCCA TGG^CCA
NaeI 360 GCCGGC GCC^GGC
NgoMIV 360 GCCGGC G^CCGGC
SacI 280 GAGCTC GAGCT^C
SacII 158 CCGCGG CCGC^GG
SapI 78 GCTCTTC GCTCTTC(1/4)
SexAI 2159 ACCTGGT A^CC[AT]GGT
XcmI 1436 CCAGCAAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Acc65I 1156 GGTACC G^GTACC
1826 GGTACC
AcyI 241 GACGCC G[GA]^CG[CT]C
1630 GACGTC
Asp718I 1156 GGTACC G^GTACC
1826 GGTACC
BbsI 1726 GAAGAC GAAGAC(2/6)
1989 GTCTTC
BpuAI 1726 GAAGAC GAAGAC(2/6)
1989 GTCTTC
BsaHI 241 GACGCC G[GA]^CG[CT]C
1630 GACGTC
BsgI 370 CTGCAC GTGCAG(16/14)
534 CTGCAC
BsrFI 360 GCCGGC [GA]^CCGG[CT]
1703 GCCGGT
BstXI 1424 CCACCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2087 CCAGCACCGTGG
Cfr10I 360 GCCGGC [GA]^CCGG[CT]
1703 GCCGGT
DraIII 1008 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
2158 CACCTGGTG
EcoT14I 883 CCTTGG C^C[AT][AT]GG
1220 CCAAGG
HgaI 241 GACGC GACGC(5/10)
2130 GACGC
Hin1I 241 GACGCC G[GA]^CG[CT]C
1630 GACGTC
KpnI 1156 GGTACC GGTAC^C
1826 GGTACC
NspI 1380 ACATGC [GA]CATG^[CT]
2107 GCATGT
PflMI 1866 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2127 CCAGACGCTGG
SmaI 351 CCCGGG CCC^GGG
488 CCCGGG
StyI 883 CCTTGG C^C[AT][AT]GG
1220 CCAAGG
Van91I 1866 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2127 CCAGACGCTGG
XmaCI 351 CCCGGG C^CCGGG
488 CCCGGG
XmaI 351 CCCGGG C^CCGGG
488 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XhoII