Restriction map for
pF1KB5787
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AvaI
AviII AvrII BamHI BbeI BbvCI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BseAI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MseI MunI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1381 GTCTAC GT^[AC][GT]AC
AflIII 2462 ACATGT A^C[GA][CT]GT
AhdI 2362 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1849 GTGCAC G^TGCAC
AlwI 951 GATCC GGATC(4/5)
AocI 460 CCTGAGG CC^T[ACGT]AGG
ApaLI 1849 GTGCAC G^TGCAC
AspEI 2362 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2086 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1288 CACGTG CAC^GTG
BciVI 1836 GGATAC GTATCC(6/5)
BclI 1895 TGATCA T^GATCA
BfaI 1456 CTAG C^TAG
BglII 281 AGATCT A^GATCT
BsaAI 1288 CACGTG [CT]AC^GT[GA]
BsaBI 1656 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1439 GAGACC GGTCTC(1/5)
BsaWI 818 ACCGGA [AT]^CCGG[AT]
BsgI 1036 GTGCAG GTGCAG(16/14)
BsiEI 1503 CGACCG CG[GA][CT]^CG
BsmBI 26 CGTCTC CGTCTC(1/5)
BsmI 1017 GAATGC GAATGC(1/-1)
BspLU11I 2462 ACATGT A^CATGT
BsrDI 2192 GCAATG GCAATG(2/0)
BssSI 11 CTCGTG CACGAG(-5/-1)
Bsu36I 460 CCTGAGG CC^T[ACGT]AGG
DrdI 1798 GACACAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2362 GACGCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 460 CCTGAGG CC^T[ACGT]AGG
FbaI 1895 TGATCA T^GATCA
HaeII 147 GGCGCT [GA]GCGC^[CT]
HincII 1783 GTCAAC GT[CT]^[GA]AC
HindII 1783 GTCAAC GT[CT]^[GA]AC
MaeI 1456 CTAG C^TAG
MamI 1656 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1841 CATATG CA^TATG
NheI 1455 GCTAGC G^CTAGC
NspI 2462 ACATGT [GA]CATG^[CT]
PflFI 2086 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1288 CACGTG CAC^GTG
PmlI 1288 CACGTG CAC^GTG
SexAI 687 ACCAGGT A^CC[AT]GGT
SmlI 391 CTCAAG C^T[CT][GA]AG
Tsp509I 2274 AATT ^AATT
Tth111I 2086 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcyI 1063 GACGCC G[GA]^CG[CT]C
2362 GACGCC
BsaHI 1063 GACGCC G[GA]^CG[CT]C
2362 GACGCC
BstYI 281 AGATCT [GA]^GATC[CT]
950 AGATCC
DraIII 556 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1905 CACTGTGTG
EarI 2107 GAAGAG CTCTTC(1/4)
2122 GAAGAG
EcoNI 36 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1827 CCTGAAGCAGG
Hin1I 1063 GACGCC G[GA]^CG[CT]C
2362 GACGCC
Ksp632I 2107 GAAGAG CTCTTC(1/4)
2122 GAAGAG
MflI 281 AGATCT [GA]^GATC[CT]
950 AGATCC
PflMI 144 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1468 CCAGACAGTGG
PleI 1756 GACTC GAGTC(4/5)
680 GAGTC
PstI 55 CTGCAG CTGCA^G
1388 CTGCAG
PvuII 1487 CAGCTG CAG^CTG
1885 CAGCTG
SacI 544 GAGCTC GAGCT^C
2376 GAGCTC
Van91I 144 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1468 CCAGACAGTGG
XhoII 281 AGATCT [GA]^GATC[CT]
950 AGATCC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
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AccII AciI AfaI AluI AlwNI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XmnI