Restriction map for
pF1KB5791
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
BbeI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BpuAI BsaHI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AvrII 2442 CCTAGG C^CTAGG
BamHI 278 GGATCC G^GATCC
BbrPI 622 CACGTG CAC^GTG
BglI 1061 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2442 CCTAGG C^CTAGG
BlpI 1539 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1539 GCTAAGC GC^T[ACGT]AGC
BsaI 526 GAGACC GGTCTC(1/5)
BsaWI 900 ACCGGA [AT]^CCGG[AT]
BseRI 1268 GAGGAG GAGGAG(10/8)
BsiEI 1666 CGATCG CG[GA][CT]^CG
BspHI 629 TCATGA T^CATGA
BssSI 1763 CTCGTG CACGAG(-5/-1)
BstAPI 1187 GCATGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1539 GCTAAGC GC^T[ACGT]AGC
EarI 877 GAAGAG CTCTTC(1/4)
HincII 2262 GTCAAC GT[CT]^[GA]AC
HindII 2262 GTCAAC GT[CT]^[GA]AC
HindIII 1331 AAGCTT A^AGCTT
Ksp632I 877 GAAGAG CTCTTC(1/4)
NcoI 1001 CCATGG C^CATGG
NdeI 2450 CATATG CA^TATG
PaeR7I 523 CTCGAG C^TCGAG
PmaCI 622 CACGTG CAC^GTG
PmlI 622 CACGTG CAC^GTG
PpuMI 242 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 1666 CGATCG CGAT^CG
RcaI 629 TCATGA T^CATGA
SacI 610 GAGCTC GAGCT^C
SmaI 1370 CCCGGG CCC^GGG
SspI 160 AATATT AAT^ATT
Tsp45I 1619 GTGAC ^GT[GC]AC
XbaI 2202 TCTAGA T^CTAGA
XcmI 2211 CCAGTTACTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 523 CTCGAG C^TCGAG
XmaCI 1370 CCCGGG C^CCGGG
XmaI 1370 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 698 GGTACC G^GTACC
1406 GGTACC
AlwNI 419 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
973 CAGCACCTG
ApaI 712 GGGCCC GGGCC^C
1828 GGGCCC
Asp718I 698 GGTACC G^GTACC
1406 GGTACC
BsaAI 586 TACGTG [CT]AC^GT[GA]
622 CACGTG
BsaBI 273 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
399 GATATACATC
BsmAI 526 GAGAC GTCTC(1/5)
2039 GAGAC
BsrBI 1018 CCGCTC CCGCTC(-3/-3)
2330 GAGCGG
BstYI 278 GGATCC [GA]^GATC[CT]
1347 GGATCT
DraII 242 AGGTCCT [GA]G^G[ACGT]CC[CT]
1827 GGGGCCC
DsaI 1001 CCATGG C^C[GA][CT]GG
1179 CCACGG
EcoO109I 242 AGGTCCT [GA]G^G[ACGT]CC[CT]
1827 GGGGCCC
EcoRI 1494 GAATTC G^AATTC
1771 GAATTC
EcoT14I 1001 CCATGG C^C[AT][AT]GG
2442 CCTAGG
HgaI 1673 GACGC GACGC(5/10)
2367 GACGC
KpnI 698 GGTACC GGTAC^C
1406 GGTACC
MaeII 587 ACGT A^CGT
623 ACGT
MamI 273 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
399 GATATACATC
MflI 278 GGATCC [GA]^GATC[CT]
1347 GGATCT
PshAI 1115 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2479 GACCCGAGTC
PvuII 1044 CAGCTG CAG^CTG
2052 CAGCTG
SphI 2092 GCATGC GCATG^C
2239 GCATGC
StyI 1001 CCATGG C^C[AT][AT]GG
2442 CCTAGG
TaqI 524 TCGA T^CGA
1665 TCGA
TthHB8I 524 TCGA T^CGA
1665 TCGA
XhoII 278 GGATCC [GA]^GATC[CT]
1347 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII EaeI
EcoNI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp509I TspRI Van91I