Restriction map for
pF1KB5799
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# NO CUT ENZYMES ( 97 enzymes) #
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Acc65I AccIII AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbvCI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspLU11I
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HaeII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PstI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1882 GTAGAC GT^[AC][GT]AC
AclI 2455 AACGTT AA^CGTT
AflIII 823 ACACGT A^C[GA][CT]GT
AseI 1387 ATTAAT AT^TAAT
AsnI 1387 ATTAAT AT^TAAT
Asp700I 2454 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 2084 CACGTG CAC^GTG
BcgI 2235 CGAAGGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 175 GGATAC GTATCC(6/5)
BclI 1820 TGATCA T^GATCA
BfaI 203 CTAG C^TAG
BglI 2154 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 753 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 753 GCTCAGC GC^T[ACGT]AGC
BsaAI 2084 CACGTG [CT]AC^GT[GA]
BsmBI 1501 GAGACG CGTCTC(1/5)
BsrBI 1050 CCGCTC CCGCTC(-3/-3)
CelII 753 GCTCAGC GC^T[ACGT]AGC
CfoI 2050 GCGC GCG^C
DrdI 601 GACTATAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1460 ATGCAT ATGCA^T
FbaI 1820 TGATCA T^GATCA
HhaI 2050 GCGC GCG^C
HinP1I 2050 GCGC G^CGC
HincII 645 GTTGAC GT[CT]^[GA]AC
HindII 645 GTTGAC GT[CT]^[GA]AC
MaeI 203 CTAG C^TAG
NsiI 1460 ATGCAT ATGCA^T
NspI 2095 ACATGC [GA]CATG^[CT]
PmaCI 2084 CACGTG CAC^GTG
PmlI 2084 CACGTG CAC^GTG
PpuMI 1472 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1387 ATTAAT AT^TAAT
Psp1406I 2455 AACGTT AA^CGTT
SacI 847 GAGCTC GAGCT^C
SapI 1791 GAAGAGC GCTCTTC(1/4)
SexAI 1958 ACCAGGT A^CC[AT]GGT
SmaI 1616 CCCGGG CCC^GGG
SpeI 202 ACTAGT A^CTAGT
XmaCI 1616 CCCGGG C^CCGGG
XmaI 1616 CCCGGG C^CCGGG
XmnI 2454 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 865 GACGTC GACGT^C
1588 GACGTC
AccII 1640 CGCG CG^CG
2049 CGCG
AocI 8 CCTTAGG CC^T[ACGT]AGG
1035 CCTGAGG
ApaI 93 GGGCCC GGGCC^C
2161 GGGCCC
AvaI 1559 CCCGAG C^[CT]CG[GA]G
1616 CCCGGG
BalI 731 TGGCCA TGG^CCA
2114 TGGCCA
BsoBI 1559 CCCGAG C^[CT]CG[GA]G
1616 CCCGGG
Bsp1407I 722 TGTACA T^GTACA
1019 TGTACA
BsrGI 722 TGTACA T^GTACA
1019 TGTACA
BstUI 1640 CGCG CG^CG
2049 CGCG
BstXI 1448 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2030 CCAACACGGTGG
Bsu36I 8 CCTTAGG CC^T[ACGT]AGG
1035 CCTGAGG
DsaI 1361 CCATGG C^C[GA][CT]GG
2183 CCATGG
Eco81I 8 CCTTAGG CC^T[ACGT]AGG
1035 CCTGAGG
EcoRI 168 GAATTC G^AATTC
1901 GAATTC
HphI 657 GGTGA GGTGA(8/7)
711 TCACC
MluNI 731 TGGCCA TGG^CCA
2114 TGGCCA
MscI 731 TGGCCA TGG^CCA
2114 TGGCCA
MvnI 1640 CGCG CG^CG
2049 CGCG
NcoI 1361 CCATGG C^CATGG
2183 CCATGG
PflMI 891 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1530 CCAGGTGCTGG
PvuII 1547 CAGCTG CAG^CTG
2270 CAGCTG
SfcI 688 CTACAG C^T[GA][CT]AG
1522 CTACAG
SmlI 253 CTCAAG C^T[CT][GA]AG
1632 CTCAAG
SspBI 722 TGTACA T^GTACA
1019 TGTACA
Van91I 891 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1530 CCAGGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HindIII HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII