Restriction map for
pF1KB8507
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# NO CUT ENZYMES ( 105 enzymes) #
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AccIII AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI AspEI
AspI AviII AvrII BbeI BbrPI
BcgI BfrI BlnI BsaBI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 1981 GACGTC GACGT^C
AccI 763 GTCTAC GT^[AC][GT]AC
AcyI 1981 GACGTC G[GA]^CG[CT]C
Aor51HI 1637 AGCGCT AGC^GCT
BbsI 1561 GAAGAC GAAGAC(2/6)
BbvCI 2169 CCTCAGC CCTCAGC(-5/-2)
BciVI 2405 GTATCC GTATCC(6/5)
BclI 2643 TGATCA T^GATCA
BglI 1721 GCCTCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1561 GAAGAC GAAGAC(2/6)
BsaHI 1981 GACGTC G[GA]^CG[CT]C
BsmI 1295 GCATTC GAATGC(1/-1)
BspHI 1533 TCATGA T^CATGA
BsrBI 80 CCGCTC CCGCTC(-3/-3)
BstXI 684 CCATGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2317 GACTACCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1637 AGCGCT AGC^GCT
EcoNI 1476 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2643 TGATCA T^GATCA
HgaI 1029 GACGC GACGC(5/10)
Hin1I 1981 GACGTC G[GA]^CG[CT]C
HindIII 1933 AAGCTT A^AGCTT
NspI 2087 ACATGC [GA]CATG^[CT]
PflMI 1137 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2216 CAGCTG CAG^CTG
RcaI 1533 TCATGA T^CATGA
SexAI 2321 ACCTGGT A^CC[AT]GGT
SspI 289 AATATT AAT^ATT
Van91I 1137 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 1541 GGTACC G^GTACC
2384 GGTACC
Asp718I 1541 GGTACC G^GTACC
2384 GGTACC
AvaI 85 CCCGGG C^[CT]CG[GA]G
515 CCCGGG
BamHI 106 GGATCC G^GATCC
1914 GGATCC
BanI 1541 GGTACC G^G[CT][GA]CC
2384 GGTACC
BlpI 63 GCTGAGC GC^T[ACGT]AGC
704 GCTCAGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
704 GCTCAGC
BsaAI 1021 TACGTG [CT]AC^GT[GA]
1264 TACGTG
BsaI 1184 GAGACC GGTCTC(1/5)
2395 GAGACC
BsaWI 866 ACCGGA [AT]^CCGG[AT]
2591 ACCGGA
BsoBI 85 CCCGGG C^[CT]CG[GA]G
515 CCCGGG
BsrDI 822 CATTGC GCAATG(2/0)
68 GCAATG
BsrFI 521 ACCGGC [GA]^CCGG[CT]
2105 ACCGGC
BssSI 1906 CACGAG CACGAG(-5/-1)
2095 CACGAG
BstAPI 1320 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2070 GCAGATCATGC
CelII 63 GCTGAGC GC^T[ACGT]AGC
704 GCTCAGC
Cfr10I 521 ACCGGC [GA]^CCGG[CT]
2105 ACCGGC
DsaI 684 CCATGG C^C[GA][CT]GG
2133 CCATGG
KpnI 1541 GGTACC GGTAC^C
2384 GGTACC
NcoI 684 CCATGG C^CATGG
2133 CCATGG
SacI 912 GAGCTC GAGCT^C
2425 GAGCTC
SmaI 85 CCCGGG CCC^GGG
515 CCCGGG
XcmI 1241 CCATTGGAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2502 CCACTTCCTGTGTGG
XmaCI 85 CCCGGG C^CCGGG
515 CCCGGG
XmaI 85 CCCGGG C^CCGGG
515 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
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AccII AciI AfaI AluI AlwI
AlwNI ApaI Asp700I AspHI AvaII
BalI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI