Restriction map for
pF1KB5710
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I ApaLI
ApoI AscI AseI AsnI AspI
AvaI AviII AvrII BalI BbrPI
BcgI BciVI BclI BfrI BlnI
BsaBI BseAI BsiWI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HpaI MamI MluI
MluNI MroI MscI MseI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 685 GGTACC G^GTACC
Aor51HI 575 AGCGCT AGC^GCT
ApaI 1541 GGGCCC GGGCC^C
Asp700I 418 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 685 GGTACC G^GTACC
BamHI 1314 GGATCC G^GATCC
BbsI 906 GTCTTC GAAGAC(2/6)
BglII 1653 AGATCT A^GATCT
BlpI 16 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 16 GCTGAGC GC^T[ACGT]AGC
BpuAI 906 GTCTTC GAAGAC(2/6)
BsaAI 1801 TACGTG [CT]AC^GT[GA]
BsaWI 833 ACCGGA [AT]^CCGG[AT]
BstXI 1325 CCACACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 16 GCTGAGC GC^T[ACGT]AGC
EagI 1427 CGGCCG C^GGCCG
EarI 277 GAAGAG CTCTTC(1/4)
EclXI 1427 CGGCCG C^GGCCG
Eco47III 575 AGCGCT AGC^GCT
Eco52I 1427 CGGCCG C^GGCCG
FseI 667 GGCCGGCC GGCCGG^CC
HincII 1290 GTTGAC GT[CT]^[GA]AC
HindII 1290 GTTGAC GT[CT]^[GA]AC
HindIII 1639 AAGCTT A^AGCTT
KpnI 685 GGTACC GGTAC^C
Ksp632I 277 GAAGAG CTCTTC(1/4)
KspI 1282 CCGCGG CCGC^GG
MfeI 739 CAATTG C^AATTG
MunI 739 CAATTG C^AATTG
NspI 429 GCATGT [GA]CATG^[CT]
PflMI 1488 CCACGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 1282 CCGCGG CCGC^GG
SapI 277 GAAGAGC GCTCTTC(1/4)
SbfI 1569 CCTGCAGG CCTGCA^GG
ScaI 242 AGTACT AGT^ACT
SexAI 40 ACCTGGT A^CC[AT]GGT
SfiI 1137 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1569 CCTGCAGG CCTGCA^GG
TaqI 1730 TCGA T^CGA
TthHB8I 1730 TCGA T^CGA
Van91I 1488 CCACGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 181 CCAGGTCCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 418 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AhdI 478 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
511 GACAGCCAGTC
AocI 82 CCTCAGG CC^T[ACGT]AGG
1884 CCTGAGG
AspEI 478 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
511 GACAGCCAGTC
BbeI 953 GGCGCC GGCGC^C
2040 GGCGCC
BbvCI 550 GCTGAGG CCTCAGC(-5/-2)
969 GCTGAGG
BfaI 235 CTAG C^TAG
851 CTAG
BsaI 29 GGTCTC GGTCTC(1/5)
694 GGTCTC
BsgI 1105 GTGCAG GTGCAG(16/14)
1413 GTGCAG
BsiEI 1427 CGGCCG CG[GA][CT]^CG
1431 CGACCG
BsmI 642 GAATGC GAATGC(1/-1)
1165 GAATGC
Bsu36I 82 CCTCAGG CC^T[ACGT]AGG
1884 CCTGAGG
Eam1105I 478 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
511 GACAGCCAGTC
Eco81I 82 CCTCAGG CC^T[ACGT]AGG
1884 CCTGAGG
KasI 953 GGCGCC G^GCGCC
2040 GGCGCC
MaeI 235 CTAG C^TAG
851 CTAG
MslI 1326 CACACCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1818 CATGGTCATG
NarI 953 GGCGCC GG^CGCC
2040 GGCGCC
NcoI 1778 CCATGG C^CATGG
1832 CCATGG
SfoI 953 GGCGCC GGC^GCC
2040 GGCGCC
Tsp45I 486 GTCAC ^GT[GC]AC
1198 GTGAC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp509I TspRI XhoII