################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BseAI BseRI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MluI MroI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 313 GTCTAC GT^[AC][GT]AC AfaI 1288 GTAC GT^AC AspHI 425 GTGCTC G[AT]GC[AT]^C BanII 177 GAGCCC G[GA]GC[CT]^C BbrPI 1282 CACGTG CAC^GTG BbvCI 1882 GCTGAGG CCTCAGC(-5/-2) BcnI 942 CCGGG CC^[GC]GG BfaI 1789 CTAG C^TAG BsaAI 1282 CACGTG [CT]AC^GT[GA] BsaI 1180 GAGACC GGTCTC(1/5) BsaWI 890 ACCGGA [AT]^CCGG[AT] BsiEI 95 CGGCCG CG[GA][CT]^CG BsiHKAI 425 GTGCTC G[AT]GC[AT]^C BspHI 444 TCATGA T^CATGA BsrDI 1506 CATTGC GCAATG(2/0) BsrFI 868 GCCGGT [GA]^CCGG[CT] BstBI 2105 TTCGAA TT^CGAA Cac8I 238 GCAGGC GC[ACGT]^[ACGT]GC Cfr10I 868 GCCGGT [GA]^CCGG[CT] DraII 1004 AGGACCT [GA]G^G[ACGT]CC[CT] EagI 95 CGGCCG C^GGCCG EclXI 95 CGGCCG C^GGCCG Eco52I 95 CGGCCG C^GGCCG EcoO109I 1004 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 2055 GCGGG CCCGC(4/6) HaeII 65 GGCGCT [GA]GCGC^[CT] HincII 1495 GTTAAC GT[CT]^[GA]AC HindII 1495 GTTAAC GT[CT]^[GA]AC HpaI 1495 GTTAAC GTT^AAC MaeI 1789 CTAG C^TAG MaeII 1283 ACGT A^CGT MspA1I 225 CAGCTG C[AC]G^C[GT]G NciI 942 CCGGG CC^[GC]GG NotI 94 GCGGCCGC GC^GGCCGC NspI 150 GCATGT [GA]CATG^[CT] NspV 2105 TTCGAA TT^CGAA PflMI 1704 CCAGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1282 CACGTG CAC^GTG PmlI 1282 CACGTG CAC^GTG PpuMI 1004 AGGACCT [GA]G^G[AT]CC[CT] PstI 236 CTGCAG CTGCA^G PvuII 225 CAGCTG CAG^CTG RcaI 444 TCATGA T^CATGA RsaI 1288 GTAC GT^AC SexAI 2075 ACCAGGT A^CC[AT]GGT SfuI 2105 TTCGAA TT^CGAA Van91I 1704 CCAGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1076 CCAAGGCTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 1262 GAATTT [GA]^AATT[CT] 1976 AAATTC AlwNI 1003 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1036 CAGAATCTG ApoI 1262 GAATTT [GA]^AATT[CT] 1976 AAATTC Asp700I 1371 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1732 GAAGACCTTC BclI 1845 TGATCA T^GATCA 2081 TGATCA BsaBI 1111 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1801 GATGAATATC BsgI 1363 CTGCAC GTGCAG(16/14) 995 GTGCAG BstYI 544 GGATCT [GA]^GATC[CT] 1359 GGATCT EarI 200 CTCTTC CTCTTC(1/4) 1920 GAAGAG EcoRV 559 GATATC GAT^ATC 1696 GATATC EcoT14I 494 CCTTGG C^C[AT][AT]GG 1076 CCAAGG FbaI 1845 TGATCA T^GATCA 2081 TGATCA Ksp632I 200 CTCTTC CTCTTC(1/4) 1920 GAAGAG MamI 1111 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1801 GATGAATATC MflI 544 GGATCT [GA]^GATC[CT] 1359 GGATCT MslI 151 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2139 CATGCGAGTG SfcI 236 CTGCAG C^T[GA][CT]AG 336 CTACAG StyI 494 CCTTGG C^C[AT][AT]GG 1076 CCAAGG XhoII 544 GGATCT [GA]^GATC[CT] 1359 GGATCT XmnI 1371 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1732 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI AlwI AvaII BalI BbsI BbvI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MfeI MluNI MnlI MscI MseI MspI MunI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I