Restriction map for
pF1KB5714
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BseAI BseRI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI FspI HgaI Hin1I
HindIII KasI KpnI KspI MluI
MroI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 313 GTCTAC GT^[AC][GT]AC
AfaI 1288 GTAC GT^AC
AspHI 425 GTGCTC G[AT]GC[AT]^C
BanII 177 GAGCCC G[GA]GC[CT]^C
BbrPI 1282 CACGTG CAC^GTG
BbvCI 1882 GCTGAGG CCTCAGC(-5/-2)
BcnI 942 CCGGG CC^[GC]GG
BfaI 1789 CTAG C^TAG
BsaAI 1282 CACGTG [CT]AC^GT[GA]
BsaI 1180 GAGACC GGTCTC(1/5)
BsaWI 890 ACCGGA [AT]^CCGG[AT]
BsiEI 95 CGGCCG CG[GA][CT]^CG
BsiHKAI 425 GTGCTC G[AT]GC[AT]^C
BspHI 444 TCATGA T^CATGA
BsrDI 1506 CATTGC GCAATG(2/0)
BsrFI 868 GCCGGT [GA]^CCGG[CT]
BstBI 2105 TTCGAA TT^CGAA
Cac8I 238 GCAGGC GC[ACGT]^[ACGT]GC
Cfr10I 868 GCCGGT [GA]^CCGG[CT]
DraII 1004 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 95 CGGCCG C^GGCCG
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoO109I 1004 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 2055 GCGGG CCCGC(4/6)
HaeII 65 GGCGCT [GA]GCGC^[CT]
HincII 1495 GTTAAC GT[CT]^[GA]AC
HindII 1495 GTTAAC GT[CT]^[GA]AC
HpaI 1495 GTTAAC GTT^AAC
MaeI 1789 CTAG C^TAG
MaeII 1283 ACGT A^CGT
MspA1I 225 CAGCTG C[AC]G^C[GT]G
NciI 942 CCGGG CC^[GC]GG
NotI 94 GCGGCCGC GC^GGCCGC
NspI 150 GCATGT [GA]CATG^[CT]
NspV 2105 TTCGAA TT^CGAA
PflMI 1704 CCAGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1282 CACGTG CAC^GTG
PmlI 1282 CACGTG CAC^GTG
PpuMI 1004 AGGACCT [GA]G^G[AT]CC[CT]
PstI 236 CTGCAG CTGCA^G
PvuII 225 CAGCTG CAG^CTG
RcaI 444 TCATGA T^CATGA
RsaI 1288 GTAC GT^AC
SexAI 2075 ACCAGGT A^CC[AT]GGT
SfuI 2105 TTCGAA TT^CGAA
Van91I 1704 CCAGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1076 CCAAGGCTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 1262 GAATTT [GA]^AATT[CT]
1976 AAATTC
AlwNI 1003 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1036 CAGAATCTG
ApoI 1262 GAATTT [GA]^AATT[CT]
1976 AAATTC
Asp700I 1371 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1732 GAAGACCTTC
BclI 1845 TGATCA T^GATCA
2081 TGATCA
BsaBI 1111 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1801 GATGAATATC
BsgI 1363 CTGCAC GTGCAG(16/14)
995 GTGCAG
BstYI 544 GGATCT [GA]^GATC[CT]
1359 GGATCT
EarI 200 CTCTTC CTCTTC(1/4)
1920 GAAGAG
EcoRV 559 GATATC GAT^ATC
1696 GATATC
EcoT14I 494 CCTTGG C^C[AT][AT]GG
1076 CCAAGG
FbaI 1845 TGATCA T^GATCA
2081 TGATCA
Ksp632I 200 CTCTTC CTCTTC(1/4)
1920 GAAGAG
MamI 1111 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1801 GATGAATATC
MflI 544 GGATCT [GA]^GATC[CT]
1359 GGATCT
MslI 151 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2139 CATGCGAGTG
SfcI 236 CTGCAG C^T[GA][CT]AG
336 CTACAG
StyI 494 CCTTGG C^C[AT][AT]GG
1076 CCAAGG
XhoII 544 GGATCT [GA]^GATC[CT]
1359 GGATCT
XmnI 1371 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1732 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI CfoI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MfeI MluNI MnlI MscI
MseI MspI MunI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I