Restriction map for pF1KB5714
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BcgI        BciVI       BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaHI       BseAI       BseRI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FseI        FspI        HgaI        Hin1I       
HindIII     KasI        KpnI        KspI        MluI        
MroI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          313  GTCTAC               GT^[AC][GT]AC
AfaI         1288  GTAC                 GT^AC
AspHI         425  GTGCTC               G[AT]GC[AT]^C
BanII         177  GAGCCC               G[GA]GC[CT]^C
BbrPI        1282  CACGTG               CAC^GTG
BbvCI        1882  GCTGAGG              CCTCAGC(-5/-2)
BcnI          942  CCGGG                CC^[GC]GG
BfaI         1789  CTAG                 C^TAG
BsaAI        1282  CACGTG               [CT]AC^GT[GA]
BsaI         1180  GAGACC               GGTCTC(1/5)
BsaWI         890  ACCGGA               [AT]^CCGG[AT]
BsiEI          95  CGGCCG               CG[GA][CT]^CG
BsiHKAI       425  GTGCTC               G[AT]GC[AT]^C
BspHI         444  TCATGA               T^CATGA
BsrDI        1506  CATTGC               GCAATG(2/0)
BsrFI         868  GCCGGT               [GA]^CCGG[CT]
BstBI        2105  TTCGAA               TT^CGAA
Cac8I         238  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr10I        868  GCCGGT               [GA]^CCGG[CT]
DraII        1004  AGGACCT              [GA]G^G[ACGT]CC[CT]
EagI           95  CGGCCG               C^GGCCG
EclXI          95  CGGCCG               C^GGCCG
Eco52I         95  CGGCCG               C^GGCCG
EcoO109I     1004  AGGACCT              [GA]G^G[ACGT]CC[CT]
FauI         2055  GCGGG                CCCGC(4/6)
HaeII          65  GGCGCT               [GA]GCGC^[CT]
HincII       1495  GTTAAC               GT[CT]^[GA]AC
HindII       1495  GTTAAC               GT[CT]^[GA]AC
HpaI         1495  GTTAAC               GTT^AAC
MaeI         1789  CTAG                 C^TAG
MaeII        1283  ACGT                 A^CGT
MspA1I        225  CAGCTG               C[AC]G^C[GT]G
NciI          942  CCGGG                CC^[GC]GG
NotI           94  GCGGCCGC             GC^GGCCGC
NspI          150  GCATGT               [GA]CATG^[CT]
NspV         2105  TTCGAA               TT^CGAA
PflMI        1704  CCAGTCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1282  CACGTG               CAC^GTG
PmlI         1282  CACGTG               CAC^GTG
PpuMI        1004  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          236  CTGCAG               CTGCA^G
PvuII         225  CAGCTG               CAG^CTG
RcaI          444  TCATGA               T^CATGA
RsaI         1288  GTAC                 GT^AC
SexAI        2075  ACCAGGT              A^CC[AT]GGT
SfuI         2105  TTCGAA               TT^CGAA
Van91I       1704  CCAGTCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1076  CCAAGGCTGAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI         1262  GAATTT               [GA]^AATT[CT]
             1976  AAATTC              
AlwNI        1003  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1036  CAGAATCTG           
ApoI         1262  GAATTT               [GA]^AATT[CT]
             1976  AAATTC              
Asp700I      1371  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1732  GAAGACCTTC          
BclI         1845  TGATCA               T^GATCA
             2081  TGATCA              
BsaBI        1111  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1801  GATGAATATC          
BsgI         1363  CTGCAC               GTGCAG(16/14)
              995  GTGCAG              
BstYI         544  GGATCT               [GA]^GATC[CT]
             1359  GGATCT              
EarI          200  CTCTTC               CTCTTC(1/4)
             1920  GAAGAG              
EcoRV         559  GATATC               GAT^ATC
             1696  GATATC              
EcoT14I       494  CCTTGG               C^C[AT][AT]GG
             1076  CCAAGG              
FbaI         1845  TGATCA               T^GATCA
             2081  TGATCA              
Ksp632I       200  CTCTTC               CTCTTC(1/4)
             1920  GAAGAG              
MamI         1111  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1801  GATGAATATC          
MflI          544  GGATCT               [GA]^GATC[CT]
             1359  GGATCT              
MslI          151  CATGTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2139  CATGCGAGTG          
SfcI          236  CTGCAG               C^T[GA][CT]AG
              336  CTACAG              
StyI          494  CCTTGG               C^C[AT][AT]GG
             1076  CCAAGG              
XhoII         544  GGATCT               [GA]^GATC[CT]
             1359  GGATCT              
XmnI         1371  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1732  GAAGACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BalI        
BbsI        BbvI        BmyI        BpmI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MfeI        MluNI       MnlI        MscI        
MseI        MspI        MunI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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