Restriction map for
pF1KB5716
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AocI ApaI ApoI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BcgI BclI BfrI BlnI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 1847 GTAGAC GT^[AC][GT]AC
AgeI 982 ACCGGT A^CCGGT
AhdI 1500 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 715 GTGCAC G^TGCAC
AlwI 1061 GGATC GGATC(4/5)
Aor51HI 23 AGCGCT AGC^GCT
ApaLI 715 GTGCAC G^TGCAC
Asp700I 1764 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1500 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 421 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 176 GGCGCC GGCGC^C
BbrPI 718 CACGTG CAC^GTG
BciVI 1398 GTATCC GTATCC(6/5)
BfaI 1930 CTAG C^TAG
BglI 1043 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1565 AGATCT A^GATCT
BsaAI 718 CACGTG [CT]AC^GT[GA]
BsaBI 1956 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 982 ACCGGT [AT]^CCGG[AT]
BsiEI 861 CGGCCG CG[GA][CT]^CG
Bsp1407I 1635 TGTACA T^GTACA
BsrDI 1836 CATTGC GCAATG(2/0)
BsrGI 1635 TGTACA T^GTACA
BstAPI 1128 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 954 GGTGACC G^GT[ACGT]ACC
BstPI 954 GGTGACC G^GT[ACGT]ACC
BstXI 1349 CCAAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 861 CGGCCG C^GGCCG
Eam1105I 1500 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1412 CTCTTC CTCTTC(1/4)
EclXI 861 CGGCCG C^GGCCG
Eco47III 23 AGCGCT AGC^GCT
Eco52I 861 CGGCCG C^GGCCG
EcoO65I 954 GGTGACC G^GT[ACGT]ACC
HincII 1305 GTTGAC GT[CT]^[GA]AC
HindII 1305 GTTGAC GT[CT]^[GA]AC
KasI 176 GGCGCC G^GCGCC
Ksp632I 1412 CTCTTC CTCTTC(1/4)
MaeI 1930 CTAG C^TAG
MamI 1956 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1619 CAATTG C^AATTG
MunI 1619 CAATTG C^AATTG
NarI 176 GGCGCC GG^CGCC
PaeR7I 1495 CTCGAG C^TCGAG
PflFI 421 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1661 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 982 ACCGGT A^CCGGT
PmaCI 718 CACGTG CAC^GTG
PmlI 718 CACGTG CAC^GTG
PstI 451 CTGCAG CTGCA^G
ScaI 560 AGTACT AGT^ACT
SfoI 176 GGCGCC GGC^GCC
SgrAI 981 CACCGGTG C[GA]^CCGG[CT]G
SspBI 1635 TGTACA T^GTACA
StuI 890 AGGCCT AGG^CCT
Tth111I 421 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1661 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1110 CCAGGGTTATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1495 CTCGAG C^TCGAG
XmnI 1764 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 176 GGCGCC G[GA]^CG[CT]C
989 GGCGTC
AflIII 2061 ACATGT A^C[GA][CT]GT
2102 ACATGT
AlwNI 1053 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
1129 CAGATTCTG
AvaI 1495 CTCGAG C^[CT]CG[GA]G
1759 CCCGAG
BanII 1975 GGGCTC G[GA]GC[CT]^C
2068 GGGCTC
BbsI 1831 GAAGAC GAAGAC(2/6)
992 GTCTTC
BcnI 173 CCCGG CC^[GC]GG
825 CCGGG
BlpI 547 GCTCAGC GC^T[ACGT]AGC
675 GCTGAGC
Bpu1102I 547 GCTCAGC GC^T[ACGT]AGC
675 GCTGAGC
BpuAI 1831 GAAGAC GAAGAC(2/6)
992 GTCTTC
BsaHI 176 GGCGCC G[GA]^CG[CT]C
989 GGCGTC
BsoBI 1495 CTCGAG C^[CT]CG[GA]G
1759 CCCGAG
BspLU11I 2061 ACATGT A^CATGT
2102 ACATGT
BstYI 1061 GGATCT [GA]^GATC[CT]
1565 AGATCT
CelII 547 GCTCAGC GC^T[ACGT]AGC
675 GCTGAGC
DraIII 1077 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2028 CACCATGTG
EcoNI 255 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1150 CCTAATGGAGG
Hin1I 176 GGCGCC G[GA]^CG[CT]C
989 GGCGTC
MflI 1061 GGATCT [GA]^GATC[CT]
1565 AGATCT
NciI 173 CCCGG CC^[GC]GG
825 CCGGG
NcoI 1787 CCATGG C^CATGG
1940 CCATGG
NspI 2061 ACATGT [GA]CATG^[CT]
2102 ACATGT
PvuII 599 CAGCTG CAG^CTG
1612 CAGCTG
SfcI 451 CTGCAG C^T[GA][CT]AG
1998 CTACAG
SmlI 754 CTCAAG C^T[CT][GA]AG
1495 CTCGAG
XhoII 1061 GGATCT [GA]^GATC[CT]
1565 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BalI BanI BbvCI BbvI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I