Restriction map for
pF1KB7786
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HgaI HpaI KpnI MaeII
MamI MfeI MluI MroI MunI
NdeI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 763 CGCG CG^CG
AcyI 605 GGCGCC G[GA]^CG[CT]C
ApaI 275 GGGCCC GGGCC^C
AvaI 1457 CCCGAG C^[CT]CG[GA]G
BbeI 605 GGCGCC GGCGC^C
BbsI 1345 GAAGAC GAAGAC(2/6)
BglI 221 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1345 GAAGAC GAAGAC(2/6)
BsaHI 605 GGCGCC G[GA]^CG[CT]C
BsmAI 745 GTCTC GTCTC(1/5)
BsmI 636 GAATGC GAATGC(1/-1)
BsoBI 1457 CCCGAG C^[CT]CG[GA]G
BspMI 1149 ACCTGC ACCTGC(4/8)
BsrFI 7 GCCGGC [GA]^CCGG[CT]
BssSI 1190 CACGAG CACGAG(-5/-1)
BstUI 763 CGCG CG^CG
BstXI 1285 CCACCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 7 GCCGGC [GA]^CCGG[CT]
EcoT14I 1115 CCATGG C^C[AT][AT]GG
EcoT22I 1000 ATGCAT ATGCA^T
FauI 218 CCCGC CCCGC(4/6)
Hin1I 605 GGCGCC G[GA]^CG[CT]C
HincII 1014 GTCAAC GT[CT]^[GA]AC
HindII 1014 GTCAAC GT[CT]^[GA]AC
KasI 605 GGCGCC G^GCGCC
KspI 762 CCGCGG CCGC^GG
MslI 986 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 763 CGCG CG^CG
NaeI 7 GCCGGC GCC^GGC
NarI 605 GGCGCC GG^CGCC
NcoI 1115 CCATGG C^CATGG
NgoMIV 7 GCCGGC G^CCGGC
NheI 511 GCTAGC G^CTAGC
NsiI 1000 ATGCAT ATGCA^T
PpuMI 1500 AGGACCC [GA]G^G[AT]CC[CT]
SacII 762 CCGCGG CCGC^GG
SfoI 605 GGCGCC GGC^GCC
StyI 1115 CCATGG C^C[AT][AT]GG
TfiI 1136 GAATC G^A[AT]TC
XcmI 1106 CCACGCCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 187 GAATTT [GA]^AATT[CT]
340 AAATTT
Alw44I 416 GTGCAC G^TGCAC
1379 GTGCAC
ApaLI 416 GTGCAC G^TGCAC
1379 GTGCAC
ApoI 187 GAATTT [GA]^AATT[CT]
340 AAATTT
AvaII 1238 GGACC G^G[AT]CC
1501 GGACC
BalI 531 TGGCCA TGG^CCA
660 TGGCCA
BanI 605 GGCGCC G^G[CT][GA]CC
1258 GGCACC
BfaI 512 CTAG C^TAG
704 CTAG
BsgI 1280 CTGCAC GTGCAG(16/14)
490 GTGCAG
BstAPI 233 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
860 GCAGCAGCTGC
CfoI 606 GCGC GCG^C
1434 GCGC
DraII 275 GGGCCCT [GA]G^G[ACGT]CC[CT]
1500 AGGACCC
DsaI 762 CCGCGG C^C[GA][CT]GG
1115 CCATGG
EcoO109I 275 GGGCCCT [GA]G^G[ACGT]CC[CT]
1500 AGGACCC
HaeII 605 GGCGCC [GA]GCGC^[CT]
1433 GGCGCT
HhaI 606 GCGC GCG^C
1434 GCGC
HinP1I 606 GCGC G^CGC
1434 GCGC
HindIII 914 AAGCTT A^AGCTT
1417 AAGCTT
MaeI 512 CTAG C^TAG
704 CTAG
MluNI 531 TGGCCA TGG^CCA
660 TGGCCA
MscI 531 TGGCCA TGG^CCA
660 TGGCCA
MseI 344 TTAA T^TAA
776 TTAA
SmlI 911 CTCAAG C^T[CT][GA]AG
1211 CTCAAG
StuI 840 AGGCCT AGG^CCT
1360 AGGCCT
Tru9I 344 TTAA T^TAA
776 TTAA
Tsp45I 1102 GTCAC ^GT[GC]AC
1339 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI BamHI BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
Cfr13I DdeI DpnII EaeI EarI
Eco81I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp509I TspRI Van91I XhoII